The pattern and frequency of t(14;18) translocation and immunophenotype in Asian follicular lymphoma

Peh, Suat‐Cheng; Shaminie, J.; Tai, Yan-Chin; Tan, Ji; Gan, Gin Gin

doi:10.1111/j.1365-2559.2004.01979.x

Cited by 15 publications

(19 citation statements)

References 46 publications

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“…The ratio of grades 3a to 3b was 2.2:1. The proportion of FL grade 3b was similar to that of prior reports from Japan but was much higher than rates reported from England and Malaysia [6,24]. The t(14;18) has been considered the hallmark of FL.…”

Section: Discussionsupporting

confidence: 86%

“…However, recent FISH studies from Southeast Asia demonstrated a relatively high frequency of the bcl-2 gene rearrangement, 56-81% [9,18,24,28]; the investigators attributed the differences observed between the results of studies from the East and the West in part to technical factors. The work of Peh et al [24] supported this notion; they performed FISH on cases without a detectable t (14;18) translocation by PCR and demonstrated a bcl-2 rearrangement in an additional 4 out of 11 cases.…”

Section: Discussionmentioning

confidence: 91%

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Analysis of follicular lymphoma by dual-color fluorescence in situ hybridization

Lee

Seo

et al. 2007

Virchows Arch

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Follicular lymphoma (FL) is divided into two groups: one with the t(14;18)(q32;q21) and the other without this translocation but with other chromosomal abnormalities including t(3q27) break. The majority of FLs in Western countries have the former chromosomal changes with characteristic clinical features and low histological grades. The goal of this study was to investigate the characteristics of Korean FLs with regard to the underlying molecular defects. Sixty-one cases of FL were evaluated from two centers in Korea by immunostaining for CD10, bcl-2, and bcl-6 proteins. Fluorescence in situ hybridization was performed to detect the t(14;18) and t(3q27) break. Cases with FL grade 3 accounted for 57% of all 61 cases. The t(14;18) was detected in 43.9% of the cases studied, and the frequency was 80, 50, 34.8, and 9.1% from grade 1 through grade 3b. The t(3q27) was detected in 16.1% of cases. Cases without a t(14;18) were mainly histological grade 3 (P < 0.001) and had a tendency to have the t(3q27) and bcl-2 amplification. The incidence of t(14;18)-positive low-grade FL was found to be lower in Korea than in Western countries. The increased frequency of t(14;18)-negative grade 3 FL attributes to the lower incidence of t(14;18) in Korean FL.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 91%

Analysis of follicular lymphoma by dual-color fluorescence in situ hybridization

Lee

Seo

et al. 2007

Virchows Arch

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“…45,55 Cells harboring the t(14;18) translocation could be thus upregulated by the accumulation of secondary multiple alterations, such as viral infections. 30,45 Interestingly, in this study, we have shown a high frequency of t(14;18) translocation in SV40-positive diffuse large B-cell lymphomas. These observations suggest that the SV40 infection may 'accelerate' the tumoral transformation of B cells, mainly those harboring the t(14;18) translocation.…”

Section: Discussionmentioning

confidence: 94%

“…30 Briefly, 5-mm-thick paraffin-embedded tissue sections were cut and dried overnight at 561C, deparaffinized, and rehydrated. Antigen retrieval was performed with the appropriate buffer (Table 2) by boiling sections in a water bath for 20 min until the temperature reached 981C, and endogenous peroxidase activity was blocked with hydrogen peroxide/ methanol for 5 min.…”

Section: Classification Of Tumors Into Germinal and Non-germinal Centmentioning

confidence: 99%

Presence of simian virus 40 in diffuse large B-cell lymphomas in Tunisia correlates with germinal center B-cell immunophenotype, t(14;18) translocation, and P53 accumulation

et al. 2008

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Previously we have reported the presence of simian virus 40 DNA in 56% of diffuse large B-cell lymphomas in Tunisia. Here, we investigated the relationship between the status of simian virus 40 and t(14;18) translocation, germinal center status, and P53 and BCL2 expression to assess the clinical and biological relevance of simian virus 40 presence in diffuse large B-cell lymphomas. Therefore, we evaluated by immunohistochemistry the expression patterns of CD10, BCL6, MUM1, BCL2, and P53 in 86 diffuse large B-cell lymphomas (48 simian virus 40-positive and 38 simian virus 40-negative cases). The t(14;18) translocation was investigated by polymerase chain reaction. Immunostaining patterns for CD10, BCL6, and MUM1 were used to subclassify diffuse large B-cell lymphoma cases as germinal center or non-germinal center phenotypes. Germinal center phenotype, t(14;18), P53, and BCL2 expression were found in 71, 30, 55, and 65% of cases, respectively. Interestingly, germinal center phenotype, t(14;18), and P53 accumulation were found to be more frequent in simian virus 40-positive cases than in simian virus 40-negative ones (81, 44, 69 vs 58, 13, 37%; P ¼ 0.018, 0.002, and 0.003, respectively). However, there were no correlations between the presence of simian virus 40 and the expression of CD10, BCL6, MUM1 and BCL2, patient's age and gender, clinical stage, or the International Prognosis Index. Multivariate logistic regression analyses revealed that the germinal center phenotype, P53 accumulation, and t(14;18) were independent factors for simian virus 40 association (P ¼ 0.029, 0.006, and 0.014, respectively). There were no significant differences in overall survival regarding P53, BCL2, or t(14;18) status. However, patients with germinal center phenotype or low International Prognosis Index scores displayed a significantly better survival than those with non-germinal center phenotype or high International Prognosis Index scores (P ¼ 0.003 and 0.0001, respectively). These two prognosis factors remain independent in multivariate analyses (P ¼ 0.001 and o0.0001, respectively). Interestingly, among patients with germinal center phenotype, simian virus 40-positive subgroup displayed a significantly shorter survival than simian virus 40-negative subgroup (P ¼ 0.034). In summary, these findings support a role of simian virus 40 in the pathogenesis of diffuse large B-cell lymphomas. On other hand, they suggest that a significant proportion of diffuse large B-cell lymphoma cases with germinal center phenotype may result from early transformation by simian virus 40, mainly those harboring the t(14;18).

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“…(23) Bcl-6 is expressed in germinal center B-cells and a subset of CD4 + T cells. (27) Bcl-6 is expressed in 88% of FLs, (28) and 55-97% of de novo DLBCLs. (29) Most of the DLBCLs that had transformed from FL retained a high frequency of Bcl-2 and Bcl-6 expression, which tended to be higher than that in de novo DLBCL.…”

Section: Discussionmentioning

confidence: 99%

Diffuse large B‐cell lymphoma after transformation from low‐grade follicular lymphoma: morphological, immunohistochemical, and FISH analyses

Maeshima¹,

Omatsu²,

Nomoto

et al. 2008

Cancer Science

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(1) FL occurs over a broad range of ages, and most cases are manifested initially in lymph nodes.The risk of FL transformation has been reported as being approximately 20% at 8 years.(2,3) Transformation to DLBCL is observed frequently, with cells most commonly resembling centroblasts, (4) but occasionally resembling anaplastic large cells with CD30 expression.(5) Rare cases have transformed to Burkitt or Burkitt-like lymphoma, (6) or precursor B-lymphoblastic lymphoma/acute lymphoid leukemia.(7) Moreover, composite FL and Hodgkin lymphoma have been suggested to represent two morphologic manifestations of the same tumor clones. (8,9) In recent years, several analyses of genetic alterations that appear to affect the risk for FL transformation have been reported, including c-MYC translocation, (6) p53 mutation, (10,11) deletions of the tumor suppressor genes p15 and p16, (12,13) and chromosomal 6q23-26 and 17q aberrations.(14) However, there have been few immunohistochemical analyses of transformed FL. FL is positive for the pan-B-cell marker CD20, and frequently positive for CD10, Bcl-2 and Bcl-6, but is usually negative for the postgerminal center B-cell or plasma cell markers CD30, MUM1 and CD138, and CD5. Because only one study has demonstrated gain of CD30 expression through FL transformation, (5) we considered that more analyses were needed to clarify the immunophenotypic changes occurring during the transformation of FL to DLBCL.Since 2000 DLBCL has been subdivided into GCB and non-GCB (including the activated B-cell phenotype [ABC] and type 3 phenotype) using the cDNA microarray technique. (15,16) The GCB group shows better outcomes and includes cases with translocation (14;18)(q32;q21). For clinical practice, Hans et al.showed that a panel of immunohistochemical markers comprising CD10, Bcl-6, and MUM1 could be used on paraffin-embedded tissues to separate DLBCL into tumors with a GCB or non-GCB phenotype.(17) Davies et al. examined 35 cases of transformed FL, and found that 89% of them had a GCB phenotype and 9% had a non-GCB phenotype. (18) The translocation (14;18)(q32;q21) is present in 80-100% of FLs in Western countries, (19,20) whereas in South-East Asia, including Japan, the incidence of translocation is considerably lower: about 60%.(21) It is unclear whether FL with t(14;18) frequently transforms to DLBCL.The aim of this study was to clarify the natural history of FL, mainly in view of immunophenotypic changes through transformations. We evaluated low-grade FLs and their transformant DLBCLs using morphological, immunhistochemical, and FISH analyses to delineate the heterogeneity of DLBCL after transformation from low-grade FL. Materials and MethodsPatients. The criteria used for identification of transformed FL were those reported for aggressive B-cell lymphomas in a review based on the workshop of the XIth Meeting of the European Association for Haematopathology.(22) Briefly, it was considered that the term 'transformation' should be used only when there was morphological evidence of simultaneo...

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The pattern and frequency of t(14;18) translocation and immunophenotype in Asian follicular lymphoma

Cited by 15 publications

References 46 publications

Analysis of follicular lymphoma by dual-color fluorescence in situ hybridization

Analysis of follicular lymphoma by dual-color fluorescence in situ hybridization

Presence of simian virus 40 in diffuse large B-cell lymphomas in Tunisia correlates with germinal center B-cell immunophenotype, t(14;18) translocation, and P53 accumulation

Diffuse large B‐cell lymphoma after transformation from low‐grade follicular lymphoma: morphological, immunohistochemical, and FISH analyses

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