The Pattern and Nature of the Lymphocyte Population Response in Dengue Hemorrhagic Fever

Boonpucknavig, S; Lohachitranond, Chanida; Nimmanitya, Suchitra

doi:10.4269/ajtmh.1979.28.885

Cited by 19 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have confirmed reports by others [32][33][34][35] that the absolute lymphocyte count, CD4 and CD8 T cell subsets, and NK cell counts are significantly decreased during acute DHF.…”

Section: Discussionsupporting

confidence: 94%

See 1 more Smart Citation

Early CD69 Expression on Peripheral Blood Lymphocytes from Children with Dengue Hemorrhagic Fever

et al. 1999

View full text Add to dashboard Cite

Recent reports have demonstrated immune activation in dengue hemorrhagic fever (DHF) by cytokine and soluble receptor detection in blood. The goal of this study was to determine which cell types are activated and likely to be responsible for cytokine production. Whole blood specimens from 51 Thai children presenting within 72 h of fever onset and with detectable plasma dengue viral RNA were studied by flow cytometry. Absolute CD4 T cell, CD8 T cell, NK cell, and gammadelta T cell counts were decreased in children with DHF compared with those with dengue fever (DF) early in the course of illness. The percent of cells expressing CD69 was increased on CD8 T cells and NK cells in children who developed DHF more than in those with DF. These data directly demonstrate that cellular immune activation is present early in acute dengue and is related to disease severity.

show abstract

“…We have confirmed reports by others [32][33][34][35] that the absolute lymphocyte count, CD4 and CD8 T cell subsets, and NK cell counts are significantly decreased during acute DHF.…”

Section: Discussionsupporting

confidence: 94%

“…This finding has been reported by others [34,37,38] and was proposed by Boonpucknavig et al [33] to represent B cells. Our study confirms that there is an increase in the proportion of B cells on the day after defervescence.…”

Section: Discussionsupporting

confidence: 85%

Early CD69 Expression on Peripheral Blood Lymphocytes from Children with Dengue Hemorrhagic Fever

et al. 1999

View full text Add to dashboard Cite

show abstract

“…These findings are consistent with previous reports, although an examination for atypical lymphocytes was not done. [22][23][24] The maximum increase in the hematocrit in the DHF group was higher than 20%, and significantly higher than those in the DF group in this study, which supports the WHO definition of the disease. 17 Increased vascular permeability would allow plasma to flow out of the intravascular compartments, leading to hypovolemic shock.…”

Section: Discussionsupporting

confidence: 76%

Comparison of Clinical Features and Hematologic Abnormalities Between Dengue Fever and Dengue Hemorrhagic Fever Among Children in the Philippines

Carlos¹,

Oishi²,

M³

et al. 2005

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

To demonstrate the differences of clinical features and hematologic abnormalities between dengue fever (DF) and dengue hemorrhagic fever (DHF), 359 pediatric patients admitted St. Luke's Medical Center in Quezon City, between 1999 and 2001 in Metro Manila, and adjoining provinces the Philippines, with a laboratory-confirmed dengue virus infection were evaluated. One third of the patients had DHF, and most of these patients were without shock. Restlessness, epistaxis, and abdominal pain were more associated with DHF. The platelet count was significantly lower in the DHF group than in the DF group before and after defervescence. In the DHF patients, the hematocrit was significantly increased before defervescence, and decreased the day after due to administration of intravenous fluid. Coagulation abnormalities associated with most DHF patients were thrombocytopenia and an increased fibrinolysis, but not disseminated intravascular coagulation. We present recent data on readily obtained clinical and laboratory data that can be used for early diagnosis and consequently earlier appropriate treatment of dengue virus infections.

show abstract

“…Early studies in dengue patients showed that increases in immunoglobulin-containing cells could be observed during infection and that these cells reached maximal numbers near the time of subsidence of fever (7). It has also been shown that total CD19 ϩ B cells increase during dengue virus infection and that these increases correlate with the presence of socalled atypical lymphocytes (19).…”

mentioning

confidence: 99%

Rapid and Massive Virus-Specific Plasmablast Responses during Acute Dengue Virus Infection in Humans

Wrammert

Onlamoon

Akondy

et al. 2012

J Virol

234

268

View full text Add to dashboard Cite

h Humoral immune responses are thought to play a major role in dengue virus-induced immunopathology; however, little is known about the plasmablasts producing these antibodies during an ongoing infection. Herein we present an analysis of plasmablast responses in patients with acute dengue virus infection. We found very potent plasmablast responses that often increased more than 1,000-fold over the baseline levels in healthy volunteers. In many patients, these responses made up as much 30% of the peripheral lymphocyte population. These responses were largely dengue virus specific and almost entirely made up of IgG-secreting cells, and plasmablasts reached very high numbers at a time after fever onset that generally coincided with the window where the most serious dengue virus-induced pathology is observed. The presence of these large, rapid, and virus-specific plasmablast responses raises the question as to whether these cells might have a role in dengue immunopathology during the ongoing infection. These findings clearly illustrate the need for a detailed understanding of the repertoire and specificity of the antibodies that these plasmablasts produce. Dengue virus causes an infection with symptoms ranging from a mild fever to severe hemorrhagic fever with vascular leakage that ranges in severity from minor subcutaneous bleeding to severe gastrointestinal bleeding (5,28,34). A striking epidemiological and immunological characteristic of dengue fever (DF) is that the severe immunopathology is more likely to occur in individuals who have previously been infected with a heterologous dengue virus serotype (8,29,32). While the exact mechanism of this phenomenon remains to be fully elucidated, several hypotheses have been developed over the last few decades to explain the reason for the exacerbated pathology observed in these patients. One of the main hypotheses revolves around a mechanism referred to as antibody-dependent enhancement (ADE) (14). This hypothesis suggests that during a secondary infection, cross-reactive yet poorly cross-neutralizing antibodies produced against a previously encountered serotype will mediate an increased infectivity, in addition to altering the host range of target cells. This mechanism has been extensively studied in vitro (6,17,20), and its importance in vivo is beginning to be elucidated (2, 10, 27). Another proposed hypothesis (22,23) suggests that an enhanced infection together with a potent T cell-mediated recall response produces massive amounts of effector mediators (4, 11-13, 15, 16, 25), a so-called cytokine storm, that is responsible for the observed immunopathology. These two mechanisms are not mutually exclusive and may in fact work in concert to cause the immunopathology of dengue disease.While human T cell responses during acute dengue virus infection have been studied in some detail, much less is known about the B cell responses. Early studies in dengue patients showed that increases in immunoglobulin-containing cells could be observed during infection and that these cel...

show abstract

The Pattern and Nature of the Lymphocyte Population Response in Dengue Hemorrhagic Fever

Cited by 19 publications

References 0 publications

Early CD69 Expression on Peripheral Blood Lymphocytes from Children with Dengue Hemorrhagic Fever

Early CD69 Expression on Peripheral Blood Lymphocytes from Children with Dengue Hemorrhagic Fever

Comparison of Clinical Features and Hematologic Abnormalities Between Dengue Fever and Dengue Hemorrhagic Fever Among Children in the Philippines

Rapid and Massive Virus-Specific Plasmablast Responses during Acute Dengue Virus Infection in Humans

Contact Info

Product

Resources

About