2020
DOI: 10.1016/j.jtherbio.2020.102647
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The pattern of HSP70 gene expression, flight activity and temperature in Apis mellifera meda colonies

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Cited by 12 publications
(8 citation statements)
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“…The findings re-emphasize that the selection of appropriate reference genes is crucial to ensure the stability of the RT-qPCR analysis [ 12 , 16 , 30 ]. Otherwise, illogical misuse of the reference genes and incorrect expression patterns of the internal reference genes will affect the correct understanding of the target genes [ 18 , 49 , 50 ].…”
Section: Discussionmentioning
confidence: 99%
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“…The findings re-emphasize that the selection of appropriate reference genes is crucial to ensure the stability of the RT-qPCR analysis [ 12 , 16 , 30 ]. Otherwise, illogical misuse of the reference genes and incorrect expression patterns of the internal reference genes will affect the correct understanding of the target genes [ 18 , 49 , 50 ].…”
Section: Discussionmentioning
confidence: 99%
“…Heat shock proteins ( Hsp ) are widely documented in insects and are one of the most important indicators of heat stress tolerance in insects. Studies have shown that they may play a key role in protecting cells from damage [ 42 , 49 , 50 ]. In view of the significance of this gene, we evaluated the robustness of the chosen candidate reference genes by utilizing Hsp70 of D. citri as a target gene.…”
Section: Methodsmentioning
confidence: 99%
“…Hsp70s generally produce protective tolerance to stress through molecular chaperone, antioxidant, immune defense and anti-apoptotic effects [ 34 ]. Several Hsp70s have been identified in insects, such as N. lugens [ 23 , 24 ], Apis mellifera [ 35 ], and Liriomyza trifolii [ 36 ]. These studies revealed that Hsp70s may play a pivotal role in insect growth and development, but how Hsp70s functions as potential effectors when they are secreted into the plants is not known.…”
Section: Discussionmentioning
confidence: 99%
“…HSP70s have already been identified in insects, and research has revealed that HSP70 is one of the critical predictors of insect tolerance to heat stress, which could protect cells from damage [ 44 ]. The study of HSP70 may help elucidate the stress responses of parasitoid wasps and in addition provide insights into their potential application in natural enemy strategies [ 45 ].…”
Section: Methodsmentioning
confidence: 99%