The pea rbcS-3A enhancer-like element directs cell-specific expression in transgenic tobacco

Aoyagi, Kazuko; Kuhlemeier, Cris; Chua, Nam‐Hai

doi:10.1007/bf00339579

Cited by 41 publications

(31 citation statements)

References 20 publications

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“…This result is contrary to that reported previously for expression from rbcS, cab, psbR, pet€ and atpD promoters (Aoyagi et al, 1988;Bichler and Herrmann, 1990;Harkins etal., 1990;Stockhaus etal., 1989). The reasons for the discrepancy are not clear, and may be related to a number of factors.…”

Section: Discussioncontrasting

confidence: 97%

“…Whereas earlier studies with transgenic plants examined expression in dissected tissue and organ samples (Ha and An, 1988;Simpson eta/., 1986), the introduction of histochemical localization of reporter gene products allowed the examination of cell-specific expression (Aoyagi et a/., 1988;Bichler and Herrmann, 1990;Stockhaus eta/., 1989;Teeri eta/., 1989). Studies with the promoters from rbcS, cab, psbR (ST-LSl), pet€ (plastocyanin) and atpD (6 subunit of ATP synthase) have demonstrated high levels of expression of the reporter genes in chloroplastcontaining cells such as leaf mesophyll and chlorenchyma associated with vascular tissues (Aoyagi et a/., 1988;Bichler and Herrmann, 1990;Stockhaus et a/., 1989). Although some expression was observed in chloroplastcontaining stomatal guard cells and trichomes, all these studies reported the absence of expression from leaf epidermal cells.…”

Section: Introductionmentioning

confidence: 99%

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Expression of photosynthesis gene‐promoter fusions in leaf epidermal cells of transgenic tobacco plants

1991

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SummaryFusions of the promoter regions of the pea plastocyanin, pea ferredoxin:NADP+ reductase and tobacco rbcS genes to the p-glucuronidase (GUS) reporter gene have been introduced into tobacco via Agrobacterium-mediated transformation, and epidermal peels of the lower leaf surface of tissue-cultured and greenhouse-grown plants examined histochemically for GUS activity. For each of the constructs, GUS was detected in epidermal cells as well as in stomatal guard cells. Epidermal peels from plants in tissue culture stained more readily than those from greenhouse-grown plants. Light and electron microscopy clearly demonstrated the presence of chloroplasts in epidermal cells of tobacco leaves. These results provide further evidence for the correlation between the presence of chloroplasts and the expression of nuclear genes for photosynthesis components.

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Section: Discussioncontrasting

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Expression of photosynthesis gene‐promoter fusions in leaf epidermal cells of transgenic tobacco plants

1991

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“…The significant expression in the paravascular tissue correlates with the relatively high numbers of chloroplasts present in this tissue in tobacco. A similar vascular-associated expression in tobacco was also reported for the RbcS promoter (Aoyagi et a/., 1988).…”

Section: Discussionsupporting

confidence: 81%

Light‐regulated expression of the Arabidopsis thaliana ferredoxin A gene involves both transcriptional and post‐transcriptional processes

et al. 1993

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SummaryFerredoxin is part of the photosynthetic apparatus of the chloroplast and is encoded in the nucleus. In Arabidopsis thaliana expression of the ferredoxin A gene is influenced by both the presence of chloroplasts and light. Tobacco plants transformed with a ferredoxin promoter-GUS fusion gene showed a tissue-specific and light-dependent expression pattern. The effect of light on the expression of the fusion gene in transgenic seedlings was only two-to fourfold, which is less pronounced than the 20-fold effect in Arabidopsis itself. Run-on transcription assays with nuclei isolated from Arabidopsis revealed a twofold modulation of transcriptional activity of the ferredoxin A gene under the influence of light. These results suggest the involvement of post-transcriptional processes in lightregulated gene expression.A ferredoxin promoter deletion series ranging from -1205 to -143 was studied. All but the smallest deletion construct (at position -143 relative to the translation start site) showed comparable expression levels in mature leaves, suggesting the presence of a positive regulating element between -269 and -143. The same pattern of tissue specificity was found in all promoter deletions studied. Expression of the fusion genes is high in all chloroplast-containing cells: mesophyll, chlorenchyma, paravascular tissue, epidermal and stomata1 guard cells and trichomes. Transgenic seedlings treated with norflurazon, which blocks the development of green chloroplasts, showed a two-to fourfold reduction in GUS expression for all constructs. In Arabidopsis seedlings the effect of norflurazon on the expression of the ferredoxin A was eightfold. This again can be explained by the need for post-transcriptional processes of the regulated gene expression of Arabidopsis ferredoxin A.

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“…This enhancer as well as the enhancer from the light-regulated rbcS-3A gene promoter, which confers both light-regulated and organ-specific expression to a reporter gene (Aoyagi et al, 1988), resemble PE-AB in being multiqualitative plant gene enhancers.…”

Section: Discussionmentioning

confidence: 99%

A two-component nodule-specific enhancer in the soybean N23 gene promoter.

1991

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The two positive cis elements in the soybean nodulin N23 gene promoter were investigated in transgenic Lofus corniculatus plants and shown to constitute a two-component nodule-specific enhancer. Equal quantitative contributions from the two components were suggested by the similar expression level of chimeric N23-chloramphenicol acetyltransferase genes after deletion of either the dista1 positive element (PE-A, -320 to -298) or the proximal positive element (PE-B, -257 to -165). A combined effect of the two elements was indicated by orientationdependent effects in the N23 promoter, and by the observation that neither PE-A nor PE-B separately was able to confer any activity to the cauliflower mosaic virus 35s minimal promoter. Reactivation of the minimal N23 and the minimal cauliflower mosaic virus 35s promoters by the inverted complete element (PE-AB) further suggested that PE-AB is a nodule-specific enhancer containing two equally strong enhancer components. Two 12-bp sequence motifs, lnvA and InvB, constituting an inverted repeat, were identified as the core of the enhancer components PE-A and PE-B, respectively. Point mutations in lnvA or lnvB resulted in lower expression levels and mutations in both abolished enhancer activity. Point mutations in two nodulin consensus sequences, 5'-CTCTT and 5'-AAAGAT located downstream of PE-AB, resulted in a decreased level of expression, confirming the involvement of these two motifs in nodulin gene expression. The binding site for the nodule-specific frans-acting factor, NAT2, present in the PE-A segment, was removed without affecting expression significantly. This interaction is, therefore, dispensable for enhancer activity.

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The pea rbcS-3A enhancer-like element directs cell-specific expression in transgenic tobacco

Cited by 41 publications

References 20 publications

Expression of photosynthesis gene‐promoter fusions in leaf epidermal cells of transgenic tobacco plants

Expression of photosynthesis gene‐promoter fusions in leaf epidermal cells of transgenic tobacco plants

Light‐regulated expression of the Arabidopsis thaliana ferredoxin A gene involves both transcriptional and post‐transcriptional processes

A two-component nodule-specific enhancer in the soybean N23 gene promoter.

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