The peanut root exudate increases the transport and metabolism of nutrients and enhances the plant growth-promoting effects of burkholderia pyrrocinia strain P10

Abstract

Background Burkholderia pyrrocinia strain P10 is a plant growth-promoting rhizobacterium (PGPR) that can substantially increase peanut growth. However, the mechanisms and pathways involved in the interaction between B. pyrrocinia P10 and peanut remain unclear. To clarify complex plant–PGPR interactions and the growth-promoting effects of PGPR strains, the B. pyrrocinia P10 transcriptome changes in response to the peanut root exudate (RE) were elucidated and the effects of RE components on biofilm formation and indole-3-acetic acid (IAA) secretion were analyzed. Results During the early interaction phase, the peanut RE enhanced the transport and metabolism of nutrients, including carbohydrates, amino acids, nitrogen, and sulfur. Although the expression of flagellar assembly-related genes was down-regulated, the expression levels of other genes involved in biofilm formation, quorum sensing, and Type II, III, and VI secretion systems were up-regulated, thereby enabling strain P10 to outcompete other microbes to colonize the peanut rhizosphere. The peanut RE also improved the plant growth-promoting effects of strain P10 by activating the expression of genes associated with siderophore biosynthesis, IAA production, and phosphorus solubilization. Additionally, organic acids and amino acids were identified as the dominant components in the peanut RE. Furthermore, strain P10 biofilm formation was induced by malic acid, oxalic acid, and citric acid, whereas IAA secretion was promoted by the alanine, glycine, and proline in the peanut RE. Conclusion The peanut RE positively affects B. pyrrocinia P10 growth, while also enhancing colonization and growth-promoting effects during the early interaction period. These findings may help to elucidate the mechanisms underlying complex plant–PGPR interactions, with potential implications for improving the applicability of PGPR strains.

Modulation of the endophytic strain Kosakonia radicincitans UYSO10 proteome by sugarcane roots exudate

“…Mobility and chemotaxis. The bacterial transition from a mobile state to bio lm formation requires the expression of transcriptional regulators, which respond to environmental signals such as root exudates (Han et al 2023;Liu et al 2024). In this sense in the presence of root exudates, the strain UYSO10 up-regulated the universal stress protein G, as well as the stringent response regulator DksA and the CheZ protein (Figure 5).…”

Progress in Microbial Fertilizer Regulation of Crop Growth and Soil Remediation Research

“…Importantly, the Burkholderia P10 strain clarifies how the P10 transcriptome affects the transformation of peanut root exudates. Furthermore, it greatly amplifies the P10 strain's promotion of plant development by promoting the biosynthesis of iron carriers, the synthesis of IAA, and the expression of genes linked to phosphorus dissolution [82].…”

Identification of Conserved Pathways in Bacillus Strains Known for Plant Growth-Promoting Behavior Using a Multifaceted Computational Approach

“…Various bacterial species, including Bacillus, Burkholderia, Azospirillum, Azotobacter, Rhizobium, and Pseudomonas, have been identified as PGPR, with Bacillus, Rhizobium, and Pseudomonas being the most prominent [2][3][4][5][6][7][8][9]. PGPR has been applied to a diverse range of plants, including chickpeas [10], maize [11], peas [12], peanuts [13], rice [14], soybeans [15], sugarcane, wheat [16], and sugarbeets [17]. Their positive impact on plant growth makes them valuable allies in sustainable agriculture practices.…”