The percentage of iNKT cells among other immune cells at various clinical stages of laryngeal cancer

Klatka, Janusz; Grywalska, Ewelina; Wasiak, Magdalena; Markowicz, Justyna; Trojanowski, Piotr; Olszański, Witold; Roliński, Jacek

doi:10.5604/17322693.1200688

Cited by 5 publications

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“…Different research projects suggest that a role of T lymphocytes might be significant in tumour development [ 3 ]. iNKT cells are a new subpopulation of T cells and show cytotoxic activity against tumours [ 4 ]. iNKT cells comprise 0.005%-0.2% of CD3+ T cells [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…Type I is called “invariant” NKT cells (iNKT cells) because of the expression of a semi-invariant TCRα chain with a Vα24 Jα18 gene segment. This type has the ability to recognise α-galactosylceramide (α-GalCer, KRN7000 – a prototypic glycolipid antigen) and ganglioside antigens (GD3), which are, respectively, exogenous and endogenous glycolipid antigens [ 4 , 7 , 8 ]. The second NKT cell type are non-invariant NKT cells, which do not express the Vα14 Jα18 TCRα-chain and do not have the possibility to recognize α-GalCer.…”

Section: Introductionmentioning

confidence: 99%

“…Zrazilova Dubska et al . showed disease-free survival in patients with breast tumours that were associated with high level of NKT-like cells [ 4 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…NKT cells connect innate and adaptive immunity [ 10 ]. iNKT cells secrete cytokines, which have pro-inflammatory and anti-inflammatory effects [ 4 , 11 ]. In animal models the significance of iNKT cells in various diseases including cancer has been shown.…”

Section: Introductionmentioning

confidence: 99%

“…Those cells respond to the presence of cancer cells. However, iNKT cells are impaired and their functionality is abnormal [ 4 , 10 , 13 ]. In patients with various malignant diseases a decreased number and reduced function of iNKT cells was observed upon activation [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Subpopulations of natural killer-T-like cells before and after surgical treatment of laryngeal cancer

Klatka

Grywalska

Hymos

et al. 2017

cejoi

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IntroductionTumours connected with head and neck comprise about 5% of all tumours. The most frequent histological type of laryngeal carcinoma is squamous cell carcinoma. Different research projects suggest that the role of T lymphocytes might be significant in tumour development. iNKT cells are a new subpopulation of T cells and show cytotoxic activity against tumours. iNKT cells participate in modulating the function of other cells which have anti-tumour properties and secrete cytokines, which have pro-inflammatory and anti-inflammatory effects. In animal models the significance of iNKT cells in various diseases including cancer was shown.Aim of the studyThe aim of this study was to determine the percentages of iNKT cells, CD161+ cells, CD161– cells, iNKT CD4+ cells, and iNKT CD8+ cells, NK cells, NKT-like cells, and T cells subsets present in peripheral blood of patients with laryngeal cancer before and two months after the tumour resection, in comparison to healthy volunteers.Materials and methodsThis study included material from laryngeal patients who were treated at the Department of Otolaryngology and Laryngological Oncology (Medical University of Lublin) between 2012 and 2013. A total of 50 patients (40 men and 10 women) aged between 45 and 77 years (median age: 60 years) were enrolled. Based on the TNM classification, the patients were classified as having stage I-IV laryngeal cancer. The control group was composed of 15 healthy volunteers (12 men and three women) aged between 43 and 82 years (median age: 61 years). The protocol of the study was approved by the Local Bioethical Committee at the Medical University of Lublin.Peripheral blood samples (15 ml) from the basilic vein were collected by venipuncture using sterile, sodium heparin-treated tubes (20 units per ml of blood) and used for cytometric analyses.ResultsiNKT cells were analysed among T CD3+ cells. The percentage of CD3+ and CD3+CD4+ T cells before tumour resection was higher than in the control group, but the increase of CD3+ T cells was not significant. The T CD3+CD4+ / T CD3+CD8+ cell ratio was significantly higher than in healthy donors. After tumour resection a decreased percentage of CD3+CD4+ T cells but an increased percentage of CD8+CD3+T cells was noted. The T CD3+CD4+ / T CD3+CD8+ cell ratio was significantly higher in patients before and after the surgery than in the control group. The amount of NKT-like cells increased after resection and was significantly higher than in the control group.ConclusionsOur study exhibited the change in percentage of iNKT, NK, NKT-like cells, and T lymphocytes after tumour resection in patients with laryngeal cancer. The research explains the contribution of those cells in immunological response against tumour.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Zrazilova Dubska et al . showed disease-free survival in patients with breast tumours that were associated with high level of NKT-like cells [ 4 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Subpopulations of natural killer-T-like cells before and after surgical treatment of laryngeal cancer

Klatka

Grywalska

Hymos

et al. 2017

cejoi

Self Cite

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Immune Monitoring during Cancer Immunotherapy

Shehata,

Dogra,

Sanjabi

2024

Manual of Molecular and Clinical Laboratory Immunology

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Potentiation of Natural Killer Cells in Cancer Immunology and Reproductive Medicine

Kiran¹

2017

JCRCT

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The discovery of NK cells in more than 30 years ago, open a new era in immunology and specifically in cancer immunology and reproductive immunology. Many studies have been taken place in medicine in order to obtain new diagnostic panels and therapeutic strategies involving NK cells. High NK cell activity has been associated with miscarriage and embryo implantation failure, because these cells attack the embryo in its early development in the uterus and recognize as a foreign body.

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The percentage of iNKT cells among other immune cells at various clinical stages of laryngeal cancer

Cited by 5 publications

References 0 publications

Subpopulations of natural killer-T-like cells before and after surgical treatment of laryngeal cancer

Subpopulations of natural killer-T-like cells before and after surgical treatment of laryngeal cancer

Immune Monitoring during Cancer Immunotherapy

Potentiation of Natural Killer Cells in Cancer Immunology and Reproductive Medicine

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