The percentages of SARS-CoV-2 protein similarity and identity with SARS-CoV and BatCoV RaTG13 proteins can be used as indicators of virus origin 1ab Polyprotein · Spike protein · Envelope protein · nsp3 · orf10

Mohammed, Mohammed Elimam Ahamed; Elimam, Mohammed; Mohammed, Ahamed

doi:10.22541/au.161801130.02276270/v1

2021

DOI: 10.22541/au.161801130.02276270/v1

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The percentages of SARS-CoV-2 protein similarity and identity with SARS-CoV and BatCoV RaTG13 proteins can be used as indicators of virus origin 1ab Polyprotein · Spike protein · Envelope protein · nsp3 · orf10

Mohammed Elimam Ahamed Mohammed¹,

Mohammed Elimam²,

Ahamed Mohammed³

Abstract: Hosted fileThe percentages of SARS-CoV-2 protein similarity and identity.pdf available at https: //authorea.com/users/364082/articles/517318-the-percentages-of-sars-cov-2-proteinsimilarity-and-identity-with-sars-cov-and-batcov-ratg13-proteins-can-be-used-asindicators-of-virus-origin-1ab-polyprotein-spike-protein-envelope-protein-nsp3-orf10

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Amantadine inhibits known and novel ion channels encoded by SARS-CoV-2 in vitro

et al. 2021

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The dire need for COVID-19 treatments has inspired strategies of repurposing approved drugs. Amantadine has been suggested as a candidate, and cellular as well as clinical studies have indicated beneficial effects of this drug. We demonstrate that amantadine and hexamethylene-amiloride (HMA), but not rimantadine, block the ion channel activity of Protein E from SARS-CoV-2, a conserved viroporin among coronaviruses. These findings agree with their binding to Protein E as evaluated by solution NMR and molecular dynamics simulations. Moreover, we identify two novel viroporins of SARS-CoV-2; ORF7b and ORF10, by showing ion channel activity in a X. laevis oocyte expression system. Notably, amantadine also blocks the ion channel activity of ORF10, thereby providing two ion channel targets in SARS-CoV-2 for amantadine treatment in COVID-19 patients. A screen of known viroporin inhibitors on Protein E, ORF7b, ORF10 and Protein 3a from SARS-CoV-2 revealed inhibition of Protein E and ORF7b by emodin and xanthene, the latter also blocking Protein 3a. This illustrates a general potential of well-known ion channel blockers against SARS-CoV-2 and specifically a dual molecular basis for the promising effects of amantadine in COVID-19 treatment. We therefore propose amantadine as a novel, cheap, readily available and effective way to treat COVID-19.

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Amantadine inhibits known and novel ion channels encoded by SARS-CoV-2 in vitro

et al. 2021

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The percentages of SARS-CoV-2 protein similarity and identity with SARS-CoV and BatCoV RaTG13 proteins can be used as indicators of virus origin 1ab Polyprotein · Spike protein · Envelope protein · nsp3 · orf10

Cited by 1 publication

References 0 publications

Amantadine inhibits known and novel ion channels encoded by SARS-CoV-2 in vitro

Amantadine inhibits known and novel ion channels encoded by SARS-CoV-2 in vitro

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