Background: Online teaching has become increasingly common in higher education, of the post-pandemic era. While a traditional face-to-face lecture or offline teaching remains very important and necessary for students to learn the medical knowledge systematically, guided by the BOPPPS teaching model, combination of online and offline learning approaches has become an unavoidable trend for maximizing teaching efficiency. However, in physiological education, the effectiveness of a combined online teaching and offline teaching model remains poorly assessed. The present study aims at providing an assessment to the hybrid teaching model.Methods: The study was performed among undergraduate medical students of Class 2017-2019, taught during 2018-2020, in the Physiology course in Harbin Medical University. Based on established offline teaching model with BOPPPS components in 2018, we incorporated online teaching contents into it to form a hybrid BOPPPS teaching model (HBOPPPS, in brief), preliminarily in 2019 and completely in 2020. The effectiveness of the HBOPPPS was assessed by comparing the final examination scores of both objective (multi-choice and single answer questions) and subjective (short and long essays) questions between different classes. Results: The final examination score of students in Class 2019 (83.9±0.5) who were taught with the HBOPPPS was significantly higher than that in Class 2017 (81.1±0.6) taught with offline BOPPPS and in Class 2018 (82.0±0.5) taught with preliminary HBOPPPS, which was mainly reflected in the average subjective scores. The subjective scores of Class 2019 (41.6±0.3) and Class 2018 (41.4±0.3) were significantly higher than that of Class 2017 (38.2±0.4). In the questionnaire among students in Class 2019, 86.2% of students were satisfied with this HBOPPPS and 79.4% students thought HBOPPPS improved their learning ability. In addition, majority of the students (73.5%) believed that reproducibility of the learning content was the major advantages of the HBOPPPS and 54.2% of the students also appreciated the flexibility of HBOPPPS. Lastly, 61.7% of the students showed a preference to be taught with the HBOPPPS in future.Conclusions: HBOPPPS is an effective teaching modal and useful for enhancing effectiveness of Physiology teaching. This is attributable to the reproducibility and flexibility as well as the increased students’ learning initiatives.