The performance of CPC/PLGA and Bio‐Oss^® for bone regeneration in healthy and osteoporotic rats

Abstract

Abstract:The current study aimed to evaluate the biological performance of calcium phosphate cement (CPC) with polylactic-co-glycolic acid (PLGA) micro-particles and Bio-Oss V R in ovariectomized and healthy rats. Thirty-two Wistar rats received alternating experimental CPC/PLGA and Bio-Oss V R in femoral condyle defects in both femurs 6 weeks after ovariectomy (OVX, n 5 16) or sham operation (SHAM, n 5 16). Six weeks after OVX or SHAM surgery, bone morphology was analyzed by in vivo computed tomography (CT) to confirm osteoporotic bone condition. Analysis of bone formation and material remnants at 4 and 12 weeks after material implantation was performed by micro-CT, descriptive histology, histomorphometry and bone dynamics by fluorochrome labeling. The in vivo CT scans showed effective induction of osteoporotic bone condition by ovariectomy. Our data showed CPC/ PLGA degraded relatively faster and more steadily. However, Bio-Oss V R had significantly less material remnants and showed significantly more bone formation compared to CPC/ PLGA. Overall, our data showed relatively high amounts of CPC/PLGA for each time point, hampering new bone formation within the defect area. Osteoporotic conditions proved to significantly affect degradation rates, but did not significantly influence bone formation. An osteoporotic bone condition affects degradation of CPC/PLGA, which is vital information for its potential use in osteoporotic conditions.

Sustained‐release of sclerostin single‐chain antibody fragments using poly(lactic‐co‐glycolic acid) microspheres for osteoporotic fracture repair

“…Poly(lactide‐co‐glycolide) (PLGA) is a common component in Food and Drug Administration‐approved biocompatible copolymer with suitable mechanical properties, adjustable degradation time and nontoxicity, and is widely used in bone tissue engineering. A number of studies have employed PLGA as the artificial microsphere delivery system for cells, growth factors, and small molecule durgs (Liao et al, ; Rafiei & Haddadi, ; van Houdt, Ulrich, Jansen, & van den Beucken, ). It has been shown to effectively improve the targeting, stability and biological activity of the loading materials.…”

Suppressing inflammation and enhancing osteogenesis using novel CS‐EC@Ca microcapsules

“…33 This may be interpreted as a wound healing process with incorporation of a body foreign material rather than bone regeneration. 34 However, there are several limitations to our research. The anti-inflammatory activity of CS-EC@Ca microcapsules was shown on the mRNA level, and this study was preliminary.…”

“…During the production process, organic parts are removed from bovine bone and the remaining Ca‐containing bone structures are processed to slowly degradable granules . This may be interpreted as a wound healing process with incorporation of a body foreign material rather than bone regeneration …”

Functionalization of Ceramic Coatings for Enhancing Integration in Osteoporotic Bone: A Systematic Review

“…The loading of CPC/PLGA (60-40 wt %) materials with ALN revealed to be successful in promoting bone formation in comparison to material without pharmaceuticals. The rate of degradation of CPC/PLGA in comparison to CPC alone allowed to control the release of ALN, maximizing the local effect [40].…”