The performance of human dental pulp stem cells on different three-dimensional scaffold materials

Zhang, Weibo; Walboomers, X. Frank; Kuppevelt, Toin H. Van; Daamen, Willeke F.; Bian, Zhuan; Jansen, John A.

doi:10.1016/j.biomaterials.2006.07.013

Cited by 206 publications

(180 citation statements)

References 27 publications

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“…These cells express markers associated with the endothelium and/or the smooth muscle, such as Stromalderived factor 1 (STRO1), Vascular cell adhesion molecule 1 (VCAM1), Melanoma-associated antigen MUC18 (MUC18) and smooth muscle actin (Téclès et al, 2005). Other reports have described the presence of a lateral population of stem cells in the dental pulp (Casagrande et al, 2006;Liu et al, 2006;Rizzino, 2009;Sloan and Smith, 2007;Volponi et al, 2010;Zhang et al, 2006b (Oda et al, 2010), as presented in this work. OCT3/4, NANOG and SOX2 are indispensable for indefinite stem cell division, without affecting differentiation potential or the capacity for self-renewal.…”

Section: Discussionsupporting

confidence: 68%

Dental Pulp of the Third Molar: A New Source of Pluripotent-like Stem Cells

Atari

Gil-Recio

Fabregat

et al. 2012

Journal of Cell Science

109

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SummaryDental pulp is particularly interesting in regenerative medicine because of the accessibility and differentiation potential of the tissue. Dental pulp has an early developmental origin with multi-lineage differentiation potential as a result of its development during childhood and adolescence. However, no study has previously identified the presence of stem cell populations with embryonic-like phenotypes in human dental pulp from the third molar. In the present work, we describe a new population of dental pulp pluripotent-like stem cells (DPPSCs) that were isolated by culture in medium containing LIF, EGF and PDGF. , and they show genetic stability in vitro based on genomic analysis with a newly described CGH technique. Interestingly, DPPSCs were able to form both embryoid-body-like structures (EBs) in vitro and teratoma-like structures that contained tissues derived from all three embryonic germ layers when injected in nude mice. We examined the capacity of DPPSCs to differentiate in vitro into tissues that have similar characteristics to mesoderm, endoderm and ectoderm layers in both 2D and 3D cultures. We performed a comparative RT-PCR analysis of GATA4, GATA6, MIXL1, NANOG, OCT3/4, SOX1 and SOX2 to determine the degree of similarity between DPPSCs, EBs and human induced pluripotent stem cells (hIPSCs). Our analysis revealed that DPPSCs, hIPSC and EBs have the same gene expression profile. Because DPPSCs can be derived from healthy human molars from patients of different sexes and ages, they represent an easily accessible source of stem cells, which opens a range of new possibilities for regenerative medicine.

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Section: Discussionsupporting

confidence: 68%

Dental Pulp of the Third Molar: A New Source of Pluripotent-like Stem Cells

Atari

Gil-Recio

Fabregat

et al. 2012

Journal of Cell Science

109

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“…32,33) Tissue engineering approaches attempts to create tissue replacement by culturing autologous cells onto three-dimensional matrixes that facilitate progenitor cell migration, proliferation, and differentiation. 34) In this study, human dental follicle stem cells (hDFSCs) were first characterized and investigated for their capability to differentiate, under appropriate culture conditions, into cells able to secrete an extracellular matrix undergoing mineralization; then a synthetic HA scaffold (ENGIpore TM , Finceramica Faenza, Italy) was added to these cells in culture and the effects evaluated by electronic microscopic analysis (scanning electron microscope; SEM).…”

Section: Introductionmentioning

confidence: 99%

Tridimensional Response of human Dental Follicular Stem Cells onto a Synthetic Hydroxyapatite Scaffold

Mastrangelo

Nargi

Carone

et al. 2008

JOURNAL OF HEALTH SCIENCE

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In the last decade, extracorporeal bone tissue engineering has found more clinical applications due to the progress and new achievements in the isolation and characterization of stem cells from different sources, as well as, in controlling proliferation and differentiation in vitro. The aim of this study is to evaluate the in vitro behaviour, morphological structure and extracellular matrix synthesis of human dental follicle stem cells (hDFSCs) isolated from human dental bud, when seeded onto a synthetic hydroxyapatite (HA) scaffold (ENGIpore c ). Populations of CD29+, CD90+, CD146+ and CD166+ were sorted by FAC sorter (FACS) analysis and were cultured in osteogenic medium and then, onto the scaffold. These cells were analyzed by optical and electronic microscopy, at week 1 and 6, before and after the differentiation. Light microscopy showed an intense attachment and colonization of the HA scaffold by polygonal-shaped cells. Scanning electron microscopy after six weeks revealed a tri-dimensional organization of the cells and the presence of dense material around the cell clusters. hDFSCs showed participation in protein biosynthesis and demonstrated high proliferation on the synthetic HA scaffold.

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“…Previous in vitro results indicate that these cells, seeded onto porous collagen, porous ceramic or fibrous titanium mesh scaffolds, deposit an abundant mineralized extracellular matrix (ECM) with expression of DSPP (Dentin Sialophosphoprotein) ( 17).…”

mentioning

confidence: 99%

Morphostructural Analysis of Human Follicular Stem Cells on Highly Porous Bone Hydroxyapatite Scaffold

Tetè¹,

Mastrangelo²,

Carone³

et al. 2007

Int J Immunopathol Pharmacol

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In this study we investigated the in vitro behaviour, morphostructure and extracellular matrix synthesis of human dental follicular stem cells (hDFSCs) isolated from human dental bud, which resulted to be positive for mesenchymal markers (CD29, CD90, CD146 and CD166) by FACS analysis. Cells were analysed by light and electronic microscopy to evaluate their biological response either at week 1, that is before differentiation, or at weeks 3–6, when they had been cultured in osteogenic medium onto a highly porous natural scaffold material (Bio-Oss®). Microscopy analysis of primary culture cells showed they had a mesenchymal stem cell-like morphostructure, spindle shaped, similar to the culture of mesenchymal stem cells derived from adult bone marrow. Also, after osteogenic differentiation, these analyses indicate typical osteoblast morphostructure and reveale a tri-dimensional organization of the cells and deposition of extracellular matrix (ECM) in close contact with biomaterial. This approach would allow to personalize the scaffold for bone tissue engineering in order to accelerate the process of osteogenesis.

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The performance of human dental pulp stem cells on different three-dimensional scaffold materials

Cited by 206 publications

References 27 publications

Dental Pulp of the Third Molar: A New Source of Pluripotent-like Stem Cells

Dental Pulp of the Third Molar: A New Source of Pluripotent-like Stem Cells

Tridimensional Response of human Dental Follicular Stem Cells onto a Synthetic Hydroxyapatite Scaffold

Morphostructural Analysis of Human Follicular Stem Cells on Highly Porous Bone Hydroxyapatite Scaffold

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