The peritrophic membrane: Ultrastructural analysis and function as a mechanical barrier to microbial infection in Orgyia pseudotsugata

Brandt, Curtis R.; Adang, Michael J.; Spence, Kemet D.

doi:10.1016/0022-2011(78)90169-6

Cited by 118 publications

(64 citation statements)

References 22 publications

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“…These compounds can solubilize proteins from the chitinous structure of the peritrophic membrane present in lepidopteran larvae (Wang & Granados, 2000). The peritrophic membrane protects intestinal cells from chemical, physical and microbial agents ingested during feeding (Brandt et al ., 1978;Wang & Granados, 1998). Consequently, degradation of the peritrophic membrane affects larval feeding, developmental rate and survival.…”

Section: Discussionmentioning

confidence: 99%

Effects of an optical brightener on the development, body weight and sex ratio ofSpodoptera frugiperda(Lepidoptera: Noctuidae)

Martínez¹,

Caballero²,

Williams³

2004

Biocontrol Science and Technology

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The incorporation of stilbene-derived optical brighteners into baculovirus formulations can substantially enhance virus infectivity and persistence of inoculum in the field. We evaluated the effect of the optical brightener Tinopal UNPA-GX (Sigma Chemical Co.) on the weight, development time, adult emergence and sex ratio of the principal pest of maize in the Americas, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae). Tinopal UNPA-GX was incorporated into semi-synthetic diet at a final concentration of 0.1 or 0.05% (w/v) and larvae of S. frugiperda were individually reared on the Tinopal-treated or an untreated control diet. In both Tinopal treatments, the weights of larvae and pupae were significantly reduced by up to 36 and 13%, respectively. The duration of larval development was increased by up to 30% compared to the controls. Adult emergence was high in all treatments (:/90%) and was not affected by the presence of Tinopal in the diet. Significant differences in the proportion of adult females were observed in the 0.05% Tinopal treatment but not in the control or 0.1% Tinopal treatments. By affecting the integrity of the peritrophic membrane and the rate of sloughing of intestinal epithelial cells, natural pest populations exposed to optical brightener residues from bioinsecticide applications are likely to suffer a reduction in growth rate and other correlates of insect fitness.

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Section: Discussionmentioning

confidence: 99%

Effects of an optical brightener on the development, body weight and sex ratio ofSpodoptera frugiperda(Lepidoptera: Noctuidae)

Martínez¹,

Caballero²,

Williams³

2004

Biocontrol Science and Technology

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“…The insect gut epithelium is protected from abrasion and some pathogens by a mucus-like layer called the peritrophic matrix, although its pore size does not preclude passage of Cry proteins (Brandt et al, 1978). This layer is mostly composed of chitin but also includes heavily glycosylated proteins (Moskalyk et al, 1996).…”

Section: Specificity Level V: Crossing the Peritrophic Matrixmentioning

confidence: 99%

Specificity determinants for Cry insecticidal proteins: Insights from their mode of action

Jurat-Fuentes

Crickmore

2017

Journal of Invertebrate Pathology

153

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“…The functions of this semipermeable membrane are not entirely clear but are likely to be crucial for the protection of underlying digestive epithelial cells from bacterial damage and parasite invasion, as well as facilitating the digestive process due to the membrane's ability to partition digestive enzymes and ingested food between the endo-and ecto-PM spaces (1). The PM is the first barrier in insects encountered by a number of ingested viral and protozoal organisms (1)(2)(3)(4)(5)(6)(7)(8), some of which use blood-or tissue-feeding insects as vectors for transmission to vertebrate hosts, where these organisms cause considerable morbidity and mortality. There is evidence that the PM of insect vectors can affect the survival and virulence of these pathogenic organisms (8 -11).…”

mentioning

confidence: 99%

Characterization of a Major Peritrophic Membrane Protein, Peritrophin-44, from the Larvae of Lucilia cuprina

Elvin

Vuocolo

Pearson

et al. 1996

Journal of Biological Chemistry

172

131

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The peritrophic membrane is a semi-permeable chitinous matrix lining the gut of most insects and is thought to have important roles in the maintenance of insect gut structure, facilitation of digestion, and protection from invasion by microrganisms and parasites. Proteins are integral components of this matrix, although the structures and functions of these proteins have not been characterized in any detail. The peritrophic membrane from the larvae of the fly Lucilia cuprina, the primary agent of cutaneous myiasis in sheep, was shown to contain six major integral peritrophic membrane proteins. Two of these proteins, a 44-kDa glycoprotein (peritrophin-44) and a 48-kDa protein (peritrophin-48) together represent >70% of the total mass of the integral peritrophic membrane proteins. Peritrophin-44 was purified and its complete amino acid sequence was determined by cloning and sequencing the DNA complementary to its mRNA. The deduced amino acid sequence codes for a protein of 356 amino acids containing an amino-terminal signal sequence followed by five similar but nonidentical domains, each of approximately 70 amino acids and characterized by a specific register of 6 cysteines. One of these domains was also present in the noncatalytic regions of chitinases from Brugia malayi, Manduca sexta, and Chelonus. Peritrophin-44 has a uniform distribution throughout the larval peritrophic membrane. Reverse transcriptase-polymerase chain reaction detected the expression of peritrophin-44 in all three larval instars but only trace levels in adult L. cuprina. The protein binds specifically to tri-N-acetyl chitotriose and reacetylated chitosan in vitro. It is concluded that the multiple cysteine-rich domains in peritrophin-44 are responsible for binding to chitin, the major constituent of peritrophic membrane. Peritrophin-44 probably has roles in the maintenance of peritrophic membrane structure and in the determination of the porosity of the peritrophic membrane. This report represents the first characterization of an insect peritrophic membrane protein.

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The peritrophic membrane: Ultrastructural analysis and function as a mechanical barrier to microbial infection in Orgyia pseudotsugata

Cited by 118 publications

References 22 publications

Effects of an optical brightener on the development, body weight and sex ratio ofSpodoptera frugiperda(Lepidoptera: Noctuidae)

Effects of an optical brightener on the development, body weight and sex ratio ofSpodoptera frugiperda(Lepidoptera: Noctuidae)

Specificity determinants for Cry insecticidal proteins: Insights from their mode of action

Characterization of a Major Peritrophic Membrane Protein, Peritrophin-44, from the Larvae of Lucilia cuprina

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