The permeability effect of microcystin-RR on Escherichia coli and Bacillus subtilis

Yang, Cuiyun; ChuanHai, Xia; Shi-wei, Zhou; Liu, Yongding

doi:10.1007/s11434-010-3169-5

Cited by 7 publications

(4 citation statements)

References 31 publications

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“…Previous studies have reported that microcystin-RR generates oxidative and cell envelope stress in E . coli [ 26 , 27 , 58 ]. Based on these reports, we hypothesized that exposure to microcystin would trigger differential expression in genes that respond to oxidative and cell envelope stress and that metabolite concentrations would alter as cells respond to induced stress.…”

Section: Discussionmentioning

confidence: 99%

Microcystin-LR does not induce alterations to transcriptomic or metabolomic profiles of a model heterotrophic bacterium

et al. 2017

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Microcystins are secondary metabolites produced by several freshwater, bloom-forming cyanobacterial species. Microcystin-producing cyanobacteria co-occur with a complex community of heterotrophic bacteria. Though conflicting, studies suggest that microcystins affect the physiology of heterotrophic bacteria by inducing oxidative stress and increasing cell envelope permeability. Based on these observations, we hypothesized that exposure to microcystin should induce differential expression in genes responding to oxidative and envelope stress and trigger shifts in metabolite pools. We tested this hypothesis by exposing Escherichia coli MG1655 to 1 and 10 mg/L microcystin-LR and monitored global changes to gene expression, cellular metabolite pools, and lipid composition using RNA-sequencing and UPLC-MS. Contrary to reported studies, we observed no evidence that microcystin-LR induced oxidative or cell envelope stress in E. coli under the tested conditions. Our results suggest a potential difference in mechanism by which microcystin-LR interacts with heterotrophic bacteria vs. cyanobacteria.

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Section: Discussionmentioning

confidence: 99%

Microcystin-LR does not induce alterations to transcriptomic or metabolomic profiles of a model heterotrophic bacterium

et al. 2017

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“…It is known that microcystins cross animal cell membranes through the transmembrane solute carriers transport family OAPT (Organic Anion Polypeptide Transporters) (Hagenbuch & Meier, 2004;Fischer et al, 2005). Regarding bacteria, it has been shown that microcystins can permeate the membranes of both gram-negative and grampositive bacteria (Dixon et al, 2004;Yang et al, 2010). Therefore, we can presume that MCs might enter bacterial cells and provoke cell damage.…”

Section: Discussionmentioning

confidence: 99%

The impact of some microcystins on the growth of heterotrophic bacteria from Portuguese freshwater reservoirs

Miguéns¹,

Valério²

2015

Limnetica

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The impact of some microcystins on the growth of heterotrophic bacteria from Portuguese freshwater reservoirs Microcystins (MCs) are hepatotoxins that are abundantly produced by cyanobacteria. Studies have shown that these toxins affect many multicellular organisms that inhabit aquatic ecosystems; however, their impact on bacteria that co-occur with freshwater cyanobacteria is still unclear. In this study, the impact of the three most common variants of MCs (MCLR, MCRR, and MCYR) on the growth of heterotrophic bacteria isolated from four Portuguese reservoirs was evaluated. Some isolates were derived from freshwater sources where blooms of cyanobacteria that often contain microcystin-producing strains are frequently observed and from a reservoir where these phenomena do not occur. Morphological and molecular characterisation of the bacterial isolates was performed, and these bacteria were exposed to three different concentrations of each MC variant. The effect of MCs on bacterial growth was then evaluated. This study showed that MCLR, MCRR and MCYR can reduce the growth of some heterotrophic bacteria isolated from freshwater sources. To our knowledge, this is the first study in which the impact of several variants of MCs was evaluated in a diverse group of freshwater heterotrophic bacteria.

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“…The capability of MCs to penetrate the cell membranes of both gram-negative and gram-positive bacteria was reported by Yang et al [ 72 ], where exposure of Escherichia coli and Bacillus subtilis (isolated and purified from cyanobacterial blooms in Lake Dianchi, China) to MC-RR promoted the bacteriolytic activity of lysozyme through the increased permeability of the cellular outer membrane. The study by Miguéns and Valério [ 73 ] further indicated that different variants of MCs (MC-LR, MC-RR and MC-YR) at various concentrations (1, 10 and 1000 nM) significantly reduced the growth of heterotrophic bacteria (phyla: Firmicutes, Actinobacteria, Bacteroidetes, β-Proteobacteria and γ-Proteobacteria), which were isolated from both cyanobacteria contaminated and non-contaminated Portuguese freshwater reservoirs.…”

Section: Cyanotoxins and Interspecies Interplaymentioning

confidence: 91%

Reviewing Interspecies Interactions as a Driving Force Affecting the Community Structure in Lakes via Cyanotoxins

et al. 2021

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The escalating occurrence of toxic cyanobacterial blooms worldwide is a matter of concern. Global warming and eutrophication play a major role in the regularity of cyanobacterial blooms, which has noticeably shifted towards the predomination of toxic populations. Therefore, understanding the effects of cyanobacterial toxins in aquatic ecosystems and their advantages to the producers are of growing interest. In this paper, the current literature is critically reviewed to provide further insights into the ecological contribution of cyanotoxins in the variation of the lake community diversity and structure through interspecies interplay. The most commonly detected and studied cyanobacterial toxins, namely the microcystins, anatoxins, saxitoxins, cylindrospermopsins and β-N-methylamino-L-alanine, and their ecotoxicity on various trophic levels are discussed. This work addresses the environmental characterization of pure toxins, toxin-containing crude extracts and filtrates of single and mixed cultures in interspecies interactions by inducing different physiological and metabolic responses. More data on these interactions under natural conditions and laboratory-based studies using direct co-cultivation approaches will provide more substantial information on the consequences of cyanotoxins in the natural ecosystem. This review is beneficial for understanding cyanotoxin-mediated interspecies interactions, developing bloom mitigation technologies and robustly assessing the hazards posed by toxin-producing cyanobacteria to humans and other organisms.

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The permeability effect of microcystin-RR on Escherichia coli and Bacillus subtilis

Cited by 7 publications

References 31 publications

Microcystin-LR does not induce alterations to transcriptomic or metabolomic profiles of a model heterotrophic bacterium

Microcystin-LR does not induce alterations to transcriptomic or metabolomic profiles of a model heterotrophic bacterium

The impact of some microcystins on the growth of heterotrophic bacteria from Portuguese freshwater reservoirs

Reviewing Interspecies Interactions as a Driving Force Affecting the Community Structure in Lakes via Cyanotoxins

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