The Peroxisomal Catalase Gene in the Methylotrophic Yeast<i>Pichia methanolica</i>

Nakagawa, Tomoyuki; Yoshida, Kyoko; Takeuchi, Akihito; Ito, Takashi; Fujimura, Shuki; Matsufuji, Yoshimi; Tomizuka, Noboru; Yurimoto, Hiroya; Sakai, Yasuyoshi; Hayakawa, T.

doi:10.1271/bbb.100329

Cited by 14 publications

(5 citation statements)

References 24 publications

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“…The enzymatic detoxification of ROS is dependent on the upregulation of antioxidant genes such as peroxisomal catalase (Nakagawa et al, 2010), cytochrome c peroxidase (Demir & Koc, 2010), peroxiredoxin TSA1 (Iraqui et al, 2009), thioredoxin reductase (Demir & Koc, 2010), glutathione peroxidase (Collinson et al, 2002), glutathione reductase (Grant et al, 1996), and glucose-6-phosphate dehydrogenase (Lee et al, 2001). Upregulation of glutathi-one oxidoreductase expression in S. cerevisiae increased resistance to hydroperoxides including H 2 O 2 , and glutaredoxins were active as glutathione peroxidases (Collinson et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Increase in antioxidant gene transcripts, stress tolerance and biocontrol efficacy of Candida oleophila following sublethal oxidative stress exposure

Liu

Wisniewski

Droby

et al. 2012

FEMS Microbiol Ecol

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A pretreatment of the yeast, Candida oleophila, with 5 mM H(2)O(2) for 30 min (sublethal) increased yeast tolerance to subsequent lethal levels of oxidative stress (50 mM H(2)O(2)), high temperature (40 °C), and low pH (pH 4). Compared with non-stress-adapted yeast cells, stress-adapted cells exhibited better control of apple fruit infections by Penicillium expansum and Botrytis cinerea and had initially higher growth rates in apple wounds. Suppression subtractive hybridization analysis was used to identify genes expressed in yeast in response to sublethal oxidative stress. Transcript levels were confirmed using semiquantitative reverse transcription-PCR. Seven antioxidant genes were upregulated. The elevated expression of these genes was associated with less accumulation of reactive oxygen species and a lower level of protein and lipid oxidation under subsequent stresses. These data support the premise that induction of abiotic stress tolerance in biocontrol yeast can improve biocontrol efficacy by upregulation of genes involved in the amelioration of oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Increase in antioxidant gene transcripts, stress tolerance and biocontrol efficacy of Candida oleophila following sublethal oxidative stress exposure

Liu

Wisniewski

Droby

et al. 2012

FEMS Microbiol Ecol

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“…Gene expression for the antioxidant system in the peroxisome, such as CTA1 and PMP20 , was largely expressed under MeOH conditions, but GPX1 expression encoding cytosolic GSH peroxidase was strongly upregulated only in H‐MeOH‐grown cells. It was reported that approximately half of the amount of Cta1p in methylotrophic yeast grown on methanol were localized in the cytoplasm together with the peroxisome (Horiguchi et al ., 2001b; Nakagawa et al ., 2010b). Therefore, O. methanolica strongly induces the peroxisomal antioxidant system in the presence of methanol, and it also enhances the cytosolic antioxidant system under H‐MeOH conditions.…”

Section: Discussionmentioning

confidence: 99%

Metabolic regulation adapting to high methanol environment in the methylotrophic yeast Ogataea methanolica

Cai

Doi²,

Shimada

et al. 2021

Microbial Biotechnology

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Since methylotrophic yeasts such as Ogataea methanolica can use methanol as a sole carbon feedstock, they could be applied to produce valuable products from methanol, a next-generation energy source synthesized from natural gases, using genetic engineering tools. In this study, metabolite profiling of O. methanolica was conducted under glucose (Glc) and low and high methanol (L-and H-MeOH) conditions to show the adaptation mechanism to a H-MeOH environment. The yeast strain responded not only to the presence of methanol but also to its concentration based on the growth condition. Under H-MeOH conditions, O. methanolica downregulated the methanol utilization, glycolytic pathway and alcohol oxidase (AOD) isozymes and dihydroxyacetone synthase (DAS) expression compared with L-MeOH-grown cells. However, levels of energy carriers, such as ATP, were maintained to support cell survival. In H-MeOH-grown cells, reactive oxygen species (ROS) levels were significantly elevated. Along with increasing ROS levels, ROS scavenging system expression was significantly increased in H-MeOH-grown cells. Thus, we concluded that formaldehyde and H 2 O 2 , which are products of methanol oxidation by AOD isozymes in the peroxisome, are overproduced in H-MeOH-grown cells, and excessive ROS derived from these cells is generated in the cytosol, resulting in upregulation of the antioxidant system and downregulation of the methanol-utilizing pathway to suppress overproduction of toxic intermediates.

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“…Its deficiency can shorten the lifespan of yeast (Wawryn et al, 1999). Peroxisomal catalase (CAT) converts the oxidant hydrogen peroxide into water and oxygen (Hiltunen et al, 2003; Nakagawa et al, 2010). The major function of mitochondrial thioredoxin reductase (TrxR) is to maintain thioredoxins in a reduced state, and plays an important role in cell proliferation (Carmel‐Harel et al, 2001; Holmgren & Lu, 2010).…”

Section: Discussionmentioning

confidence: 99%

Sodium glutamate as a booster: Inducing Rhodosporidium paludigenum to enhance the inhibition of Penicillium expansum on pears

Huang

Cai

2021

J of Applied Microbiology

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Aims: This research sought to improve the ability of biocontrol yeast to suppress postharvest fungal disease and explore possible mechanisms of action. Methods and Results:The addition of 2% sodium glutamate (SG), which is edible and recognized as safe, enhances the inhibitory effect of Rhodosporidium paludigenum Fell & Tallman on Penicillium expansum in vivo and in vitro. Rhodosporidium paludigenum cells grown in medium with a final concentration of 2% SG, displayed viability under a variety of stress conditions, including sodium chloride (NaCl), calcofluor white (CFW), Congo red (CR) and sodium dodecyl sulphate (SDS). Activity and relative gene expression levels of antioxidant-related enzymes in R. paludigenum, including peroxisomal catalase (CAT), thioredoxin reductase (TrxR), glutathione peroxidase (GSH-PX), glutathione reductase (GR) and superoxide dismutase (SOD) were altered in the presence of SG. Levels of reactive oxygen species (ROS) increased in cells grown in the presence of SG as well as the content of several amino acids. Conclusions:In the presence of 2% SG R. paludigenum inhibited P. expansum and exhibited tolerance to a number of stressful conditions which may involve the upregulation of antioxidant enzymes and amino acids.

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The Peroxisomal Catalase Gene in the Methylotrophic YeastPichia methanolica

Cited by 14 publications

References 24 publications

Increase in antioxidant gene transcripts, stress tolerance and biocontrol efficacy of Candida oleophila following sublethal oxidative stress exposure

Increase in antioxidant gene transcripts, stress tolerance and biocontrol efficacy of Candida oleophila following sublethal oxidative stress exposure

Metabolic regulation adapting to high methanol environment in the methylotrophic yeast Ogataea methanolica

Sodium glutamate as a booster: Inducing Rhodosporidium paludigenum to enhance the inhibition of Penicillium expansum on pears

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