The Peroxisome Deficient Arabidopsis Mutant <i>sse1</i> Exhibits Impaired Fatty Acid Synthesis

Lin, Yuwei; Cluette‐Brown, Joanne E.; Goodman, Howard M.

doi:10.1104/pp.103.036772

Cited by 78 publications

(80 citation statements)

References 62 publications

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“…Although some reports on the metabolism of seed development have been published previously they have tended to focus on earlier stages of seed development and on specific biochemical pathways largely concentrating on sugar components Baud and Graham, 2006;Gomez et al, 2006), fatty acids and oil (Cernac and Benning, 2004;Lin et al, 2004;Vigeolas et al, 2004;Gó mez et al, 2005), or specific transcriptional programs (Hobbs et al, 2004;Braybrook et al, 2006). Broader transcriptional studies have also been previously conducted with an emphasis on the stages of development from 5 to 13 DAF (Ruuska et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis Seed Development and Germination Is Associated with Temporally Distinct Metabolic Switches

Fait

Angelovici²,

Less³

et al. 2006

Plant Physiology

407

418

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While the metabolic networks in developing seeds during the period of reserve accumulation have been extensively characterized, much less is known about those present during seed desiccation and subsequent germination. Here we utilized metabolite profiling, in conjunction with selective mRNA and physiological profiling to characterize Arabidopsis (Arabidopsis thaliana) seeds throughout development and germination. Seed maturation was associated with a significant reduction of most sugars, organic acids, and amino acids, suggesting their efficient incorporation into storage reserves. The transition from reserve accumulation to seed desiccation was associated with a major metabolic switch, resulting in the accumulation of distinct sugars, organic acids, nitrogen-rich amino acids, and shikimate-derived metabolites. In contrast, seed vernalization was associated with a decrease in the content of several of the metabolic intermediates accumulated during seed desiccation, implying that these intermediates might support the metabolic reorganization needed for seed germination. Concomitantly, the levels of other metabolites significantly increased during vernalization and were boosted further during germination sensu stricto, implying their importance for germination and seedling establishment. The metabolic switches during seed maturation and germination were also associated with distinct patterns of expression of genes encoding metabolism-associated gene products, as determined by semiquantitative reverse transcription-polymerase chain reaction and analysis of publicly available microarray data. When taken together our results provide a comprehensive picture of the coordinated changes in primary metabolism that underlie seed development and germination in Arabidopsis. They furthermore imply that the metabolic preparation for germination and efficient seedling establishment initiates already during seed desiccation and continues by additional distinct metabolic switches during vernalization and early germination.

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Section: Discussionmentioning

confidence: 99%

Arabidopsis Seed Development and Germination Is Associated with Temporally Distinct Metabolic Switches

Fait

Angelovici²,

Less³

et al. 2006

Plant Physiology

407

418

View full text Add to dashboard Cite

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“…To confirm the identity of peroxisomes, a further construct was made encoding monomeric red fluorescent protein (mRFP) with a PTS1 sequence at the C-terminal end ( Figure 3A). This PTS1 comprised the 10 C-terminal amino acids of pumpkin malate synthase that had previously been verified to transport RFP to peroxisomes in Arabidopsis (Lin et al, 2004). Plants were transformed with GFP and mRFP transgenes and then crossed and fluorescence observed in the root hairs of seedlings expressing both fluorescent proteins ( Figure 3B).…”

Section: Identification Of Peroxisomal Csysmentioning

confidence: 99%

“…Confocal microscopy was performed to observe GFP and mRFP in root hair cells as previously described (Lin et al, 2004). For transmission electron microscopy, cotyledons from seedlings germinated for 5 d on half-strength MS medium supplemented with 1% (w/v) sucrose were processed as previously described (Germain et al, 2001) …”

Section: Microscopymentioning

confidence: 99%

“…A CSY3 cDNA was obtained from Riken (pda02633; Seki et al, 2002) and subcloned within the CaMV cassette to generate p35S:CSY3. A modified CSY3 cDNA was generated in which the first 60 bases, corresponding to the PTS2 signal sequence, were deleted and a PTS1 sequence, corresponding to the last 10 amino acids of the pumpkin MLS gene Lin et al, 2004) were fused at the 39 end. This construct was called p35S:DCSY3-SRL.…”

Section: Plasmid Constructionmentioning

confidence: 99%

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Arabidopsis Peroxisomal Citrate Synthase Is Required for Fatty Acid Respiration and Seed Germination

2005

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We tested the hypothesis that peroxisomal citrate synthase (CSY) is required for carbon transfer from peroxisomes to mitochondria during respiration of triacylglycerol in Arabidopsis thaliana seedlings. Two genes encoding peroxisomal CSY are expressed in Arabidopsis seedlings, and seeds from plants with both CSY genes disrupted were dormant and did not metabolize triacylglycerol. Germination was achieved by removing the seed coat and supplying sucrose, but the seedlings still did not use triacylglycerol. The mutant seedlings were resistant to 2,4-dichlorophenoxybutyric acid, indicating a block in peroxisomal b-oxidation, and were unable to develop further after transfer to soil. The mutant phenotype was complemented with a cDNA encoding CSY with either its native peroxisomal targeting sequence (PTS2) or a heterologous PTS1 sequence from pumpkin (Cucurbita pepo) malate synthase. These results suggest that peroxisomal CSY in Arabidopsis is not only a key enzyme of the glyoxylate cycle but also catalyzes an essential step in the respiration of fatty acids. We conclude that citrate is exported from the peroxisome during fatty acid respiration, whereas in yeast, acetylcarnitine is exported.

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“…However, seed protein content was unaffected. A mutation in the Shrunken Seed1 (SSE1) gene altered seed composition by increasing starch and reducing oil and protein content (Lin et al, 2004). Mutations in metabolic pathways are also capable of dramatic changes in seed composition.…”

mentioning

confidence: 99%

System Analysis of an Arabidopsis Mutant Altered in de Novo Fatty Acid Synthesis Reveals Diverse Changes in Seed Composition and Metabolism

et al. 2009

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Embryo-specific overexpression of biotin carboxyl carrier protein 2 (BCCP2) inhibited plastid acetyl-coenzyme A carboxylase (ACCase), resulting in altered oil, protein, and carbohydrate composition in mature Arabidopsis (Arabidopsis thaliana) seed. To characterize gene and protein regulatory consequences of this mutation, global microarray, two-dimensional difference gel electrophoresis, iTRAQ, and quantitative immunoblotting were performed in parallel. These analyses revealed that (1) transgenic overexpression of BCCP2 did not affect the expression of three other ACCase subunits; (2) four subunits to plastid pyruvate dehydrogenase complex were 25% to 70% down-regulated at protein but not transcript levels; (3) key glycolysis and de novo fatty acid/lipid synthesis enzymes were induced; (4) multiple storage proteins, but not cognate transcripts, were upregulated; and (5) the biotin synthesis pathway was up-regulated at both transcript and protein levels. Biotin production appears closely matched to endogenous BCCP levels, since overexpression of BCCP2 produced mostly apo-BCCP2 and the resulting ACCase-compromised, low-oil phenotype. Differential expression of glycolysis, plastid pyruvate dehydrogenase complex, fatty acid, and lipid synthesis activities indicate multiple, complex regulatory responses including feedback as well as futile "feed-forward" elicitation in the case of fatty acid and lipid biosynthetic enzymes. Induction of storage proteins reveals that oil and protein synthesis share carbon intermediate(s) and that reducing malonyl-coenzyme A flow into fatty acids diverts carbon into amino acid and protein synthesis.

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The Peroxisome Deficient Arabidopsis Mutant sse1 Exhibits Impaired Fatty Acid Synthesis

Cited by 78 publications

References 62 publications

Arabidopsis Seed Development and Germination Is Associated with Temporally Distinct Metabolic Switches

Arabidopsis Seed Development and Germination Is Associated with Temporally Distinct Metabolic Switches

Arabidopsis Peroxisomal Citrate Synthase Is Required for Fatty Acid Respiration and Seed Germination

System Analysis of an Arabidopsis Mutant Altered in de Novo Fatty Acid Synthesis Reveals Diverse Changes in Seed Composition and Metabolism

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