The pH Dependence of the Individual Steps in the Glucose Oxidase Reaction

Bright, Harold J.; Appleby, M.

doi:10.1016/s0021-9258(18)83415-9

Cited by 218 publications

(70 citation statements)

References 15 publications

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“…(1-Hydroxy-3-aminopropyl)ferrocene (12). Solid cyanoalcohol 38 13 (9.0 g, 35.3 mmol) was added in small portions to a suspension of LiAlH 4 (1.61 g, 42.4 mmol) in Et 2 O (250 cm 3 ) and the resulting mixture was stirred at room temperature for 3 h. The reaction mixture was worked-up according to the procedure described above for the aminoalcohol (1).…”

Section: 1ј-dimethyl-3-(2-amino-1-hydroxyethyl)ferrocenementioning

confidence: 99%

“…8 The subunits are linked by two disulfide bonds 1, 9 with the two remaining cysteine residues each being located in the flavin binding sites as inferred from studies with 4-thioflavin probes. 10 The mechanism of action of the enzyme (on glucose) has been analysed in some detail 11, 12 and has been found to follow the "ping-pong" mechanism 13 whereby the product, δ-gluconolactone, is released before binding with oxygen (and other electron acceptors). The enzyme is very specific for β-glucose; α--glucose, mannose and galactose are oxidized but at rates 100 times slower.…”

Section: Introductionmentioning

confidence: 99%

“…A study of the pH dependence of the enzyme reaction led to the proposal that a carboxylate group has a catalytic function within the active site. 12 The crystal structure of partially deglycosylated GOx from Asp. niger has been determined.…”

Section: Introductionmentioning

confidence: 99%

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The influence of structure in the reaction of electrochemically generated ferrocenium derivatives with reduced glucose oxidase

Forrow¹,

Sanghera²,

Walters³

2002

J. Chem. Soc., Dalton Trans.

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The synthesis and characterisation of a series of ferrocenylaminoalcohols is reported. 1,2-Aminoalcohol compounds were prepared from the respective ferrocene aldehydes via reaction with trimethylsilylcyanide followed by reduction with LiAlH 4 . This series includes the ferrocene derivative 1,1Ј-dimethyl-3-(2-amino-1-hydroxyethyl)ferrocene 1, which is used as a redox mediator to glucose oxidase in a commercial biosensor for determining blood glucose levels in diabetics. The aminoalcohol derivatives are included in a structure-activity study involving the electrochemical determination of the mediation rates of a range of systematically substituted ferrocenes with glucose oxidase. These mediation rates are correlated with structure. DALTON

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Section: 1ј-dimethyl-3-(2-amino-1-hydroxyethyl)ferrocenementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The influence of structure in the reaction of electrochemically generated ferrocenium derivatives with reduced glucose oxidase

Forrow¹,

Sanghera²,

Walters³

2002

J. Chem. Soc., Dalton Trans.

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“…As the final step, optimum pH value was analyzed. Since it is known that the GOx is an active enzyme in the range of pH 3.0 and pH 8.0 [13] different 50 mM buffer solutions having pH values as 6.0-8.0 were prepared. The best sensor performance was obtained with pH 7.5 (Figure 1D).…”

Section: Optimization Studiesmentioning

confidence: 99%

INVESTIGATION THE EFFECT OF DIOCTYLFLUORENYL-co- BENZOTHIADIAZOLE BEARING POLYMER AND MWCNTS ON BIOSENSOR PERFORMANCE

Söylemez

2019

Mugla Journal of Science and Technology

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The combination of conjugated polymers and carbon nanotubes serve great contributions in many research fields because of their superior characteristics. Herein, an electrochemical biosensor, using poly [(9,9-dioctylfluorenyl-2,7-diyl)-alt-co-(1,4-benzo-{2,1',3}-thiadiazole)] (PFBTz) and multi-walled carbon nanotubes (MWCNTs) was constructed for glucose sensing. To create such a surface, a graphite electrode was coated with PFBTz and MWCNTs, respectively. Then, glucose oxidase (GOx) was immobilized onto this new platform using glutaraldehyde (GA) as the cross linker. The limit of detection, linear range and sensitivity values were found to be 0.018 mM, 0.025-0.6 mM and 66.0 μAmM -1 cm -2 , respectively. The selectivity of the prepared biosensor was also examined in the presence of interferences and as a result, it has been observed that the biosensor response was much higher for glucose in all measurements. Surface characterizations before and after enzyme immobilization were done using scanning electron microscopy (SEM) and cyclic voltammetry (CV) techniques. In the final part of the study, the constructed biosensor was applied to beverages for glucose detection and very promising results were obtained. With this work, a robust, novel and economic sensing platform was prepared for glucose determination.

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“…This is consistent with other estimates of these rate constants for GOx with glucose as a substrate. 12,31,38,61 The dependence of the scaled variable…”

Section: Sigmoid Response Achieved By Neutralizing Some Of the Inputmentioning

confidence: 99%

Modeling and Modifying Response of Biochemical Processes for Biocomputing and Biosensing Signal Processing

Domanskyi

Privman

2016

Emergence, Complexity and Computation

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Processes involving multi-input multi-step reaction cascades are used in developing novel biosensing, biocomputing, and decision making systems. In various applications different changes in responses of the constituent processing steps (reactions) in a cascade are desirable in order to allow control of the system's response. Here we consider conversion of convex response to sigmoid by "intensity filtering," as well as "threshold filtering," and we offer a general overview of this field of research. Specifically, we survey rate equation modelling that has been used for enzymatic reactions. This allows us to design modified biochemical processes as "network components" with responses desirable in applications.

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The pH Dependence of the Individual Steps in the Glucose Oxidase Reaction

Cited by 218 publications

References 15 publications

The influence of structure in the reaction of electrochemically generated ferrocenium derivatives with reduced glucose oxidase

The influence of structure in the reaction of electrochemically generated ferrocenium derivatives with reduced glucose oxidase

INVESTIGATION THE EFFECT OF DIOCTYLFLUORENYL-co- BENZOTHIADIAZOLE BEARING POLYMER AND MWCNTS ON BIOSENSOR PERFORMANCE

Modeling and Modifying Response of Biochemical Processes for Biocomputing and Biosensing Signal Processing

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