The Phantom Menace for Patients with Hepatobiliary Diseases: &lt;i&gt;Shewanella haliotis&lt;/i&gt;, Often Misidentified as &lt;i&gt;Shewanella algae&lt;/i&gt; in Biochemical Tests and MALDI-TOF Analysis

Byun, Jung Hyun; Park, Hyun Woong; Kim, Sun‐Joo

doi:10.7883/yoken.jjid.2015.658

Cited by 21 publications

(24 citation statements)

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“…State-of-the-art methods can discriminate between S. algae and S. putrefaciens on the basis of their biochemical and phenotypic characteristics [ 1 ]. Similar tests have been reported, though, to be inconclusive for discrimination of S. algae from other emerging pathogenic Shewanella , thereby requiring molecular techniques that include 16S rRNA and/or gyrB DNA sequencing for accurate species identification [ 39, 40 ]. Byun et al .…”

Section: Discussionmentioning

confidence: 78%

“…Byun et al . reported the inability to discriminate between S. algae and S. haliotis on the basis of biochemical and phenotypic assays [ 39 ]. Even 16S rRNA sequencing, not routinely conducted in the clinical microbiology laboratory, can be insufficient to determine the identity of an isolate at the species level (E. Fernández-Fernández et al ., unpublished results).…”

Section: Discussionmentioning

confidence: 99%

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Shewanella spp. infections in Gran Canaria, Spain: retrospective analysis of 31 cases and a literature review

et al. 2017

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IntroductionShewanella spp. can cause severe skin and soft-tissue infections, gastrointestinal infections, otitis and bacteraemia, generally upon contact with seawater or consumption of raw seafood. Recently, a new condition termed ‘patera foot’ characterized by acute skin and soft-tissue infection has been described in irregular immigrants arriving to the Canary Islands, Spain, in rudimentary boats. Most infections are caused by a single species, Shewanella algae. The improvement of the diagnostic capabilities in clinical microbiology laboratories has resulted in a growing number of cases being reported worldwide, most of them coming from warm regions.Case presentationIn this work, we reviewed the medical records of all the patients with Shewanella infections in the two university hospitals of Gran Canaria (the Canary Islands, Spain) during the period 2000–2016, resulting in the identification of 31 cases. We also conducted a literature review of Shewanella infections reported worldwide in recent years.ConclusionThis case series suggests that Shewanella infections are nosocomially acquired more frequently than previously thought. In addition, the unexpectedly high proportion of multidrug-resistant isolates raises concerns.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Shewanella spp. infections in Gran Canaria, Spain: retrospective analysis of 31 cases and a literature review

et al. 2017

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“…was only detected in fish showing clinical signs of a disease, namely S. algae and S. seohaensis . Reports on infections of human patients with S. algae , S. haliotis , S. putrefaciens and S. xiamenensis are published, and in these reports, the detection of S. algae exceeded those of S. putrefaciens (Byun, Park, & Kim, ). One reason for a higher virulence of S. algae might be the possible production of tetrodotoxin by this species (Simidu, Kita‐Tsukamoto, Yasumoto, & Yotsu, ; Simidu, Noguchi, Hwang, Shida, & Hashimoto, ).…”

Section: Discussionmentioning

confidence: 99%

Methods for identification and differentiation of different Shewanella spp. isolates for diagnostic use

Jung‐Schroers

Jung

Ryll

et al. 2017

Journal of Fish Diseases

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Shewanella spp. are Gram-negative, rod-shaped, motile bacteria that are widely distributed in marine and freshwater environments. The bacteria are present in the physiological microflora of fish from temperate waters and are known as fish spoilage species. From clinically healthy fish and from fish with skin ulcerations, Shewanella spp. is regularly isolated, indicating a possible role as fish pathogen. In this study, 74 isolates of Shewanella spp. were analysed. For species identification, biochemical techniques, 16S rRNA sequencing, MALDI-TOF MS and the Sherlock Microbial Identification System (MIS) based on the composition of fatty acid ethyl esters were compared. The phylogenetic relationship, cytotoxicity in vitro and resistance against antibiotics were tested. The most reliable method for species identification was 16S rRNA sequencing. From diseased fish, clinically healthy fish and the aquatic environment, different Shewanella species were isolated. This indicates that Shewanella spp. is widespread in the aquatic milieu and acts as a secondary pathogen. The virulence of Shewanella spp. is probably not depending on the species but on the isolate itself. Many isolates of Shewanella spp. were showing multiresistances against antibiotic substances, especially in samples derived from retailers and in routine diagnostics, all Shewanella spp. should therefore be tested for resistances against antibiotic agents.

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“…In Asia, Shewanella -associated infections have been reported in Thailand [1], Japan [26] and Taiwan [11] of China. Risk factors of human infections attributed to the genus Shewanella occur in warmer climates.…”

Section: Discussionmentioning

confidence: 99%

“…A retrospective investigation of Shewanella bacteremia in patients with hepatobiliary disease from Taiwan, China indicated that, five out of nine Shewanella strains were confirmed as the S. haliotis using 16S rRNA sequencing analysis [11]. Byun JH et al [26] reported 19 strains which were identified as S. algae by using VITEK 2 and Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, whereas 16S rRNA analysis identified 10 isolates as S. algae and 9 isolates as S. haliotis . The reason is that only two Shewanella species, S. putrefaciens and S. algae were registered in the database of VITEK 2 system and MALDI-TOF mass spectrometry.…”

Section: Discussionmentioning

confidence: 99%

Rare Shewanella spp. associated with pulmonary and bloodstream infections of cancer patients, China: a case report

et al. 2018

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BackgroundMembers of Shewanella species are opportunistic pathogens that are found in marine environments. Currently more than sixty species have been identified, whereas the most commonly clinical cases associated with Shewanella species have involved only two species, i.e., S. algae and S. putrefaciens. We present two cases of pulmonary and bloodstream infections caused by two rare Shewanella spp. strains from patients of gastrointestinal cancer.Case presentationTwo male patients with a history of gastrointestinal cancer presented to hospital with pulmonary and bloodstream infections, respectively. The infective pathogens of both cases were primarily isolated and identified as Shewanella algae (case I) and Shewanella putrefaciens (case II) by phenotypic features and VITEK 2 system, but they were further confirmed as Shewanella haliotis and Shewanella upenei by 16S rRNA gene sequence analysis. The major bacterial composition of the bronchoalveolar lavage in case I was also identified as Shewanella by 16S rRNA amplicon sequencing analysis. Antimicrobial susceptibility testing showed that the two strains had broad susceptibility, but S. haliotis in the case I was resistant to ciprofloxacin and levofloxacin and S. upenei in the case II was intermediate to imipenem, piperacillin/tazobactam and ciprofloxacin.ConclusionsTo the best of our knowledge, this is the first cases of the pulmonary and bloodstream infections caused by Shewanella spp. from clinical patients in mainland China. Shewanella as a potential pathogen in China should not be ignored.Electronic supplementary materialThe online version of this article (10.1186/s12879-018-3354-8) contains supplementary material, which is available to authorized users.

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The Phantom Menace for Patients with Hepatobiliary Diseases: <i>Shewanella haliotis</i>, Often Misidentified as <i>Shewanella algae</i> in Biochemical Tests and MALDI-TOF Analysis

Cited by 21 publications

References 14 publications

Shewanella spp. infections in Gran Canaria, Spain: retrospective analysis of 31 cases and a literature review

Shewanella spp. infections in Gran Canaria, Spain: retrospective analysis of 31 cases and a literature review

Methods for identification and differentiation of different Shewanella spp. isolates for diagnostic use

Rare Shewanella spp. associated with pulmonary and bloodstream infections of cancer patients, China: a case report

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