The Phaseolus vulgaris ZIP gene family: identification, characterization, mapping, and gene expression

Abstract

Zinc is an essential mineral for humans and plants and is involved in many physiological and biochemical processes. In humans, Zn deficiency has been associated with retarded growth and reduction of immune response. In plants, Zn is an essential component of more than 300 enzymes including RNA polymerase, alkaline phosphatase, alcohol dehydrogenase, Cu/Zn superoxidase dismutase, and carbonic anhydrase. The accumulation of Zn in plants involves many genes and characterization of the role of these genes will be useful in biofortification. Here we report the identification and phlyogenetic and sequence characterization of the 23 members of the ZIP (ZRT, IRT like protein) family of metal transporters and three transcription factors of the bZIP family in Phaseolus vulgaris L. Expression patterns of seven of these genes were characterized in two bean genotypes (G19833 and DOR364) under two Zn treatments. Tissue analyzed included roots and leaves at vegetative and flowering stages, and pods at 20 days after flowering. Four of the genes, PvZIP12, PvZIP13, PvZIP16, and Pv bZIP1, showed differential expression based on tissue, Zn treatment, and/or genotype. PvZIP12 and PvZIP13 were both more highly expressed in G19833 than DOR364. PvZIP12 was most highly expressed in vegetative leaves under the Zn (−) treatment. PvZIP16 was highly expressed in leaf tissue, especially leaf tissue at flowering stage grown in the Zn (−) treatment. Pv bZIP1 was most highly expressed in leaf and pod tissue. The 23 PvZIP genes and three bZIP genes were mapped on the DOR364 × G19833 linkage map. PvZIP12, PvZIP13, and PvZIP18, Pv bZIP2, and Pv bZIP3 were located near QTLs for Zn accumulation in the seed. Based on the expression and mapping results, PvZIP12 is a good candidate gene for increasing seed Zn concentration and increase understanding of the role of ZIP genes in metal uptake, distribution, and accumulation of zinc in P. vulgaris.

A node‐localized transporter OsZIP3 is responsible for the preferential distribution of Zn to developing tissues in rice

“…They are characterized by a similar membrane topology, in which the amino and carboxyl termini of the proteins are located on the outside surface of the plasma membrane (Guerinot, ). Many ZIP members have been reported in plants, for example 15 members in Arabidopsis (Mäser et al ., ), 17 in rice (Chen et al ., ) and 23 in common bean ( Phaseolus vulgaris L.) (Astudillo et al ., ). Some of these have been characterized in terms of localization, expression pattern, and transport activity.…”

Metal transporter encoding gene families in Fabaceae: III. The zinc-iron permease (ZIP) gene family

“…AtIRT1 (Iron-regulate transporter 1) là gene ZIP đầu tiên được xác định 6 , mã hóa protein có chức năng vận chuyển sắt ở bề mặt rễ của cây A.thaliana 7 . Theo thời gian, họ gene ZIP đã bước đầu được nghiên cứu ở các loài A.thaliana 4,8 , lúa (Oryza sativa), dương (Populus trichocarpa), nho (Vitis vinifera) 9 , ngô (Zea mays) 10 và cả một số loài cây họ Đậu như cỏ thập tự ba lá (M. truncatula) 11 và đậu Cove (Phaseolus vulgaris) 12 .…”

“…Phương pháp tìm kiếm gene tương đồng trong nghiên cứu này dựa vào các gene ZIP của cây đậu Cove. Tổng số 23 gene ZIP được sử dụng làm trình tự truy vấn (query sequence) 12 . Cây phả hệ, các đặc điểm lí hóa cũng như sự biểu hiện các gene ZIP được phân tích bằng phương pháp tin sinh học 13 .…”

Biofortification: how can we exploit plant science and biotechnology to reduce micronutrient deficiencies?

“…Three different gene families have been described in detail and proposed as possible targets for future Zn or Fe biofortification strategies: the NAC transcription factors (Ricachenevsky et al, 2013a), the metal tolerance proteins (MTP's) (Ricachenevsky et al, 2013b) and the ZIP family of metal transporters (Astudillo et al, 2013). Notably, a candidate gene for increasing Zn concentration in common bean, PvZIP12 , is proposed in Astudillo et al (2013) and a model has been proposed for the role of the rice OsNAC5 gene in senescence and metal re-mobilization (Ricachenevsky et al, 2013a).…”

“…Notably, a candidate gene for increasing Zn concentration in common bean, PvZIP12 , is proposed in Astudillo et al (2013) and a model has been proposed for the role of the rice OsNAC5 gene in senescence and metal re-mobilization (Ricachenevsky et al, 2013a). …”