The PHD transcription factor Cti6 is involved in the fungal colonization and aflatoxin B1 biological synthesis of Aspergillus flavus

Zhang, Mengjuan; Gao, Lin; Pan, Xiaohua; Song, Weiqun; Tan, Cheng; Chen, Xuan; Yu, Yang; Zhang, Zhuang

doi:10.1186/s43008-021-00062-2

Cited by 16 publications

(3 citation statements)

References 37 publications

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“…The mycelia were grounded into powder with liquid nitrogen and 1.0 mL TRIzol reagent was added (Biomarker Technologies, Beijing, China). The total RNA was extracted according to previous protocols. , Genomic DNA was digested by DNase I (Promega Corporation, USA), and RNA quality was evaluated with a NanoDrop 2000 spectrophotometer (Thermo Fisher, Waltham, Massachusetts, USA). The RNA was then reverse transcribed into cDNA using the PrimeScript RT-PCR Kit (Takara, China).…”

Section: Methodsmentioning

confidence: 99%

Synthesis, Characterization, and Antifungal Activity of Benzimidazole-Grafted Chitosan against Aspergillus flavus

Liu,

Chen,

et al. 2024

J. Agric. Food Chem.

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Aspergillus flavus contamination in agriculture and food industries poses threats to human health, leading to a requirement of a safe and effective method to control fungal contamination. Chitosan-based nitrogen-containing derivatives have attracted much attention due to their safety and enhanced antimicrobial applications. Herein, a new benzimidazole-grafted chitosan (BAC) was synthesized by linking the chitosan (CS) with a simple benzimidazole compound, 2-benzimidazolepropionic acid (BA). The characterization of BAC was confirmed by Fourier transform infrared (FTIR) spectroscopy and nuclear magnetic resonance spectroscopy ( 1 H and 13 C NMR). Then, the efficiency of BAC against A. flavus ACCC 32656 was investigated in terms of spore germination, mycelial growth, and aflatoxin production. BAC showed a much better antifungal effect than CS and BA. The minimum inhibitory concentration (MIC) value was 1.25 mg/mL for BAC, while the highest solubility of CS (16.0 mg/mL) or BA (4.0 mg/ mL) could not completely inhibit the growth of A. flavus. Furthermore, results showed that BAC inhibited spore germination and elongation by affecting ergosterol biosynthesis and the cell membrane integrity, leading to the permeabilization of the plasma membrane and leakage of intracellular content. The production of aflatoxin was also inhibited when treated with BAC. These findings indicate that benzimidazole-derived natural CS has the potential to be used as an ideal antifungal agent for food preservation.

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Section: Methodsmentioning

confidence: 99%

Synthesis, Characterization, and Antifungal Activity of Benzimidazole-Grafted Chitosan against Aspergillus flavus

Liu,

Chen,

et al. 2024

J. Agric. Food Chem.

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“…To detect subcellular localisation of Afngg1, A. flavus spore suspensions (10 6 ) were inoculated into Dextrose Peptone Yeast (DPY) liquid medium and cultured at 30 °C for 24 h. After collection, mycelia were washed with phosphate-buffered saline (PBS), stained with 4,6-diamidino-2-phenylindole for 10 min according to a previous method [ 57 ], and observed with an FV1000 laser confocal microscope (Olympus, Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

Histone 2-Hydroxyisobutyryltransferase Encoded by Afngg1 Is Involved in Pathogenicity and Aflatoxin Biosynthesis in Aspergillus flavus

Wang

Liang

Shan

et al. 2022

Toxins

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Aflatoxin, a carcinogenic secondary metabolite produced by Aspergillus flavus, is a significant threat to human health and agricultural production. Histone 2-hydroxyisobutyrylation is a novel post-translational modification that regulates various biological processes, including secondary metabolism. In this study, we identified the novel histone 2-hydroxyisobutyryltransferase Afngg1 in A. flavus, and explored its role in cell growth, development and aflatoxin biosynthesis. Afngg1 gene deletion markedly decreased lysine 2-hydroxyisobutyrylation modification of histones H4K5 and H4K8 compared with the control strain. Additionally, Afngg1 deletion inhibited mycelial growth of A. flavus, and the number of conidia and hydrophobicity were significantly decreased. Notably, aflatoxin B1 biosynthesis and sclerotia production were completely inhibited in the ΔAfngg1 strain. Furthermore, the pathogenicity of the ΔAfngg1 strain infecting peanut and corn grains was also diminished, including reduced spore production and aflatoxin biosynthesis compared with A. flavus control and Afngg1 complementation strains. Transcriptome analysis showed that, compared with control strains, differentially expressed genes in ΔAfngg1 were mainly involved in chromatin remodelling, cell development, secondary metabolism and oxidative stress. These results suggest that Afngg1 is involved in histone 2-hydroxyisobutyrylation and chromatin modification, and thus affects cell development and aflatoxin biosynthesis in A. flavus. Our results lay a foundation for in-depth research on the 2-hydroxyisobutyrylation modification in A. flavus, and may provide a novel target for aflatoxin contamination prevention.

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“…The fungal spores (10 6 /mL) were cultured in PDB medium for 48 h, and then mycelium was ground into powder with liquid nitrogen. Total RNA was prepared by TRIpure total RNA Extraction Reagent (Bestek, China) according to the protocol used by Zhang [47] . qRT-PCR was performed according to previously described [48] , and the primers were shown in Table 3.…”

Section: Quantitative Rt-pcr (Qrt-pcr) Analysismentioning

confidence: 99%

SntB triggers the antioxidant pathways to regulate development and aflatoxin biosynthesis inAspergillus flavus

Wu,

Yang,

Yao

et al. 2023

Preprint

Self Cite

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The epigenetic reader SntB was identified as an important transcriptional regulator of growth, development, and secondary metabolite synthesis inAspergillus flavus. However, the underlying molecular mechanism is still unclear. In this study,sntBgene deletion (ΔsntB), complementary (Com-sntB), and HA tag fused tosnt2(snt2-HA) strains were constructed by using the homologous recombination method, respectively. Our results revealed that deletion ofsntBinhibited the processes of mycelia growth, conidial production, sclerotia formation, aflatoxin synthesis, and ability to colonize host compared to wild type (WT), and the defective phenotype of knockout strain ΔsntBcan be restored by its complementary strain Com-sntB. Chromatin immunoprecipitation sequencing (ChIP-seq) ofsntB-HA and WT and RNA sequencing (RNA-seq) of ΔsntBand WT strains revealed that SntB played key roles in oxidative stress response ofA. flavus. The function ofcatC(encode a catalase) gene was further analyzed based on the integration results of ChIP-seq and RNA-seq. In ΔsntBstrain, the relative expression level ofcatCwas significantly higher than in WT strain, while a secretory lipase encoding gene (G4B84_008359) was down-regulated. Under the stress of oxidant menadione sodium bisulfite (MSB), the deletion ofsntBobvious down-regulated the expression level ofcatC. After deletion ofcatCgene, the mycelia growth, conidial production, and sclerotia formation were inhibited, while aflatoxin synthesis was increased compared to the WT strain. Results also showed that the inhibition rate of MSB to ΔcatCstrain was significantly lower than that of WT group and AFB1 yield of the ΔcatCstrain was significantly decreased than that of WT strain under the stress of MSB. Our study revealed the potential machinery that SntB regulated fungal morphogenesis, mycotoxin anabolism, and fungal virulence through the axle of from SntB to fungal virulence and mycotoxin bio-synthesis, i.e. H3K36me3 modification-SntB-Peroxisomes-Lipid hydrolysis-fungal virulence and mycotoxin bio-synthesis. The results of the study shad light into the SntB mediated epigenetic regulation pathway of fungal mycotoxin anabolism and virulence, which provided potential strategy for control the contamination ofA. flavusand its aflatoxins.

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The PHD transcription factor Cti6 is involved in the fungal colonization and aflatoxin B1 biological synthesis of Aspergillus flavus

Cited by 16 publications

References 37 publications

Synthesis, Characterization, and Antifungal Activity of Benzimidazole-Grafted Chitosan against Aspergillus flavus

Synthesis, Characterization, and Antifungal Activity of Benzimidazole-Grafted Chitosan against Aspergillus flavus

Histone 2-Hydroxyisobutyryltransferase Encoded by Afngg1 Is Involved in Pathogenicity and Aflatoxin Biosynthesis in Aspergillus flavus

SntB triggers the antioxidant pathways to regulate development and aflatoxin biosynthesis inAspergillus flavus

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