The phenolic substances of manufactured tea. I.—Fractionation and paper chromatography of water‐soluble substances

Roberts, E. A. H.; Cartwright, Reed A.; Oldschool, M.

doi:10.1002/jsfa.2740080203

Cited by 140 publications

(77 citation statements)

References 13 publications

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“…The phytochemical screening of the plant extracts was performed by thin layer chromatography (TLC) according to Harborne [16] and Roberts et al [17]. Aliquots of ten microliters of the extracts of the extracts were applied on silica gel chromatography plates, using elution systems and suitable developers to investigate the presence of saponins, flavonoids, cinnamic derivatives, phenylpropanoids, triterpenoids, steroids, mono-and sesquiterpenes, alkaloids, proanthocyanidins and leucoanthocyanidins, coumarins, and quinones.…”

Section: Phytochemical Analysismentioning

confidence: 99%

Antioxidant Activity and Phytochemical Profile of <i>Spondias tuberosa</i> Arruda Leaves Extracts

Uchôa¹,

Oliveira²,

Pereira³

et al. 2015

AJPS

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Spondias tuberosa Arruda (umbuzeiro), a Brazilian semiarid plant, is a species of great economic, social, and ecological importance. In folk medicine, the leaves have been used in the treatment of diabetes, inflammation, stomach and uterine pains, and constipation. In this study, the antioxidant properties of ethyl acetate and methanol leaves extracts were evaluated in vitro using different methods: free radicals elimination by DPPH and ABTS assays, and transition metal reduction by phosphomolybdenium assay. In addition, a phytochemical study was also carried out. The methanolic leaves extracts showed the strongest antioxidant activity and the higher values for total phenolic and flavonoids. The results showed that S. tuberosa leaves have antioxidant activity and this seems to be related to the phenolic content.

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Section: Phytochemical Analysismentioning

confidence: 99%

Antioxidant Activity and Phytochemical Profile of <i>Spondias tuberosa</i> Arruda Leaves Extracts

Uchôa¹,

Oliveira²,

Pereira³

et al. 2015

AJPS

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“…Polyphenol fractions were obtained by ethyl acetate extraction and lead salt precipitation (Roberts, Cartwright & Oldschool, 1957;Vuataz & Brandenburger, 1961), and were analysed by two-dimensional chromatography (Roberts et al 1957). 'Tannin' was determined by the Lowenthal method (Jacobs, 1958), caffeine spectrophotometrically (Polzella, 1961), and phytic acid by the method of McCance & Widdowson (1935).…”

Section: Organic Componentsmentioning

confidence: 99%

The composition of tea infusions examined in relation to the association between mortality and water hardness

Anderson

Hollins

Bond

1971

J. Hyg.

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SUMMARYRecent epidemiological studies have shown that death-rates from certain chronic diseases are higher in areas with soft than in areas with hard drinkingwater. In the striking negative correlation found in the county boroughs of England and Wales between cardiovascular mortality and water hardness the important underlying factor is apparently the water calcium. Interest is therefore focused on the dietary significance of calcium present in drinking-water. In relation to that interest, the present report gives a quantitative account of the composition of tea infusions prepared with waters containing different amounts of calcium. It is shown that a substantial part of water calcium is taken up by the tea leaf during the preparation of infusions. The analysis of the infusions covers a wide range of individual components, including trace metals and polyphenolic substances. It appears that the principal change caused in infusion composition by the presence of calcium in the water is a substantial reduction in the relatively high oxalate content. The question is raised whether there may be some connexion between the 'water factor' in cardiovascular disease and the absorption of oxalates from foods.

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“…For partial purification of secondary metabolites, 10 μL aliquots of biomass extract ( , and specific visualization reagents were used (Robertson et al, 1956;Metz, 1961;Sharma and Darwra, 1991;Wagner and Bladt, 1996;Harbone, 1998). The chromatograms were run in saturated chambers.…”

Section: Secondary Metabolic Analysesmentioning

confidence: 99%

Antimicrobial activity of Phoma sp. URM 7221: An endophyte from Schinus terebinthifolius Raddi (Anacardiaceae)

Silva¹,

Silvino²,

Bezerra³

et al. 2017

Afr. J. Microbiol. Res.

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The discovery of new metabolites potentially bioactive against pathogenic microorganisms, mainly multidrug resistant, has aroused interest in endophytic fungi. The plant-associated microorganisms have been an important source for development of new compounds of biotechnological interest. This study aimed to investigate the antibacterial capacity of the endophytic fungus, Phoma sp. URM 7221 isolated from the medicinal plant Schinus terebinthifolius against human-pathogenic bacteria. An endophyte was isolated from S. terebinthifolius leaves. Phoma herbarum URM7221 was characterized morphologically and on the basis of ITS rDNA sequence. Primary antimicrobial activity was evaluated using the agar diffusion method and fermentation in liquid medium. Six different solvents were used to extract the active metabolites from fungal biomass and metabolic liquid. An antimicrobial activity test from the extract was carried out using a disk diffusion method with the endophytic extract containing the best antibacterial activity. Two tests were performed: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Partially purified secondary metabolite extracts were analysed by thin layer chromatography (TLC). Liquid metabolically bioactive compounds extracted with petroleum ether revealed a MIC of 25 and 500 μg.mL −1 against S. aureus and MRSA, respectively. Ether and methanol extracts were assessed by chemical analyses and contained phenolic compounds, triterpenes, steroids, reducing sugars, mono-and sesquiterpenes. The thin layer chromatography assay showed the activity of different antimicrobial compounds produced by Phoma sp. URM7221. This endophyte (URM7221) could be efficiently used for production of bioactive metabolites against pathogenic microorganisms, with significant biotechnological potential.

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The phenolic substances of manufactured tea. I.—Fractionation and paper chromatography of water‐soluble substances

Cited by 140 publications

References 13 publications

Antioxidant Activity and Phytochemical Profile of <i>Spondias tuberosa</i> Arruda Leaves Extracts

Antioxidant Activity and Phytochemical Profile of <i>Spondias tuberosa</i> Arruda Leaves Extracts

The composition of tea infusions examined in relation to the association between mortality and water hardness

Antimicrobial activity of Phoma sp. URM 7221: An endophyte from Schinus terebinthifolius Raddi (Anacardiaceae)

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The phenolic substances of manufactured tea. I.—Fractionation and paper chromatography of water‐soluble substances

Cited by 140 publications

References 13 publications

Antioxidant Activity and Phytochemical Profile of &lt;i&gt;Spondias tuberosa&lt;/i&gt; Arruda Leaves Extracts

Antioxidant Activity and Phytochemical Profile of &lt;i&gt;Spondias tuberosa&lt;/i&gt; Arruda Leaves Extracts

The composition of tea infusions examined in relation to the association between mortality and water hardness

Antimicrobial activity of Phoma sp. URM 7221: An endophyte from Schinus terebinthifolius Raddi (Anacardiaceae)

Contact Info

Product

Resources

About

Antioxidant Activity and Phytochemical Profile of <i>Spondias tuberosa</i> Arruda Leaves Extracts

Antioxidant Activity and Phytochemical Profile of <i>Spondias tuberosa</i> Arruda Leaves Extracts