The Phenols in the Wax and in the Suberin Polymer of Green Cotton Fibres and Their Functions

Schmutz, Alain; Buchala, Antony; Ryser, Ulrich; Jenny, Titus A.

doi:10.17660/actahortic.1994.381.33

Cited by 13 publications

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“…Changes in the glycerol concentration of the wax and suberin fractions induced by two specific inhibitors of suberin biosynthesis were correlated with changes in the concentrations of the aliphatic suberin monomers (Schmutz et al, 1993(Schmutz et al, , 1994a(Schmutz et al, , 1994b(Schmutz et al, , 1996.…”

Section: Discussionmentioning

confidence: 97%

Glycerol Is a Suberin Monomer. New Experimental Evidence for an Old Hypothesis1

et al. 1999

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The monomer composition of the esterified part of suberin can be determined using gas chromatography-mass spectroscopy technology and is accordingly believed to be well known. However, evidence was presented recently indicating that the suberin of green cotton (Gossypium hirsutum cv Green Lint) fibers contains substantial amounts of esterified glycerol. This observation is confirmed in the present report by a sodium dodecyl sulfate extraction of membrane lipids and by a developmental study, demonstrating the correlated accumulation of glycerol and established suberin monomers. Corresponding amounts of glycerol also occur in the suberin of the periderm of cotton stems and potato (Solanum tuberosum) tubers. A periderm preparation of wound-healing potato tuber storage parenchyma was further purified by different treatments. As the purification proceeded, the concentration of glycerol increased at about the same rate as that of ␣, -alkanedioic acids, the most diagnostic suberin monomers. Therefore, it is proposed that glycerol is a monomer of suberins in general and can cross-link aliphatic and aromatic suberin domains, corresponding to the electrontranslucent and electron-opaque suberin lamellae, respectively. This proposal is consistent with the reported dimensions of the electron-translucent suberin lamellae.Suberin and cutin are insoluble, lipophilic biopolymers. Together with complex mixtures of soluble lipids, they form the protective layers of higher plants: the cuticle of the epidermis and the suberin layers of the periderm and the exodermis. These cell layers are diffusion barriers for water and other

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Section: Discussionmentioning

confidence: 97%

Glycerol Is a Suberin Monomer. New Experimental Evidence for an Old Hypothesis1

et al. 1999

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“…Flavonoid and hydroxycinnamic acid metabolites usually accumulate in the central vacuoles of guard cells and epidermal cells as well as subepidermal cells of leaves (Weissenböck, Hedrich & Sachs 1986; Hutzler et al 1998) where they protect the inner cell layers from UV‐B damage (Landry, Chapple & Last 1995; Sheahan 1996). In addition, some compounds were found to be covalently linked to plant cell walls (Strack et al 1988; Schnitzler et al 1996), others occur in waxes (Schmutz et al 1994) or on external surfaces of plant organs (Cuadra & Harborne 1996; Chaves, Escudero & Gutierrez‐Merino 1997).…”

Section: Introductionmentioning

confidence: 99%

Seasonal accumulation of ultraviolet‐B screening pigments in needles of Norway spruce (Picea abies (L.) Karst.)

Fischbach¹,

Kossmann²,

Panten³

et al. 1999

Plant Cell & Environment

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“…Flavonoids can usually accumulate in epidermal tissue (Day, ; Hutzler et al, ; Manetas, ), waxes (Schmutz, Buchala, Ryser, & Jenny, ; Karabourniotis & Liakopoulos, 2005), cell walls (Schnitzler et al, ), vacuoles (Suzuki, Yamazaki, Sada, Oguni, & Moriyasu, ), nucleus (Feucht, Treutter, Dithmar, & Polster, ; Saslowsky, Warek, & Winkel, ). Until now, the localization of flavonoids in Apocyni Veneti Folium has not been well studied.…”

Section: Introductionmentioning

confidence: 99%

Site‐specific accumulation and dynamic change of flavonoids in Apocyni Veneti Folium

Chen

Liu

et al. 2017

Microscopy Res & Technique

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Site-specific accumulation of flavonoids in Apocyni Veneti Folium was determined by laser scanning confocal microscope (LSCM) and the localization of catechins also was observed via vanillin-HCl staining under the conventional optical microscope. The contents of five flavonoids in Apocyni Veneti Folium from different harvest times and growth parts were measured using HPLC method. LSCM observation showed that flavonoids are accumulated in cuticle of epidermal cells and vessel walls, especially in protoplasts and nucleolus of the collenchyma cells and the epidermal cells. Catechins are localized in the palisade parenchyma cells and vessel walls, particularly in the laticifers found in the phloem. On the basis of the difference of the maximal emission wavelength between quercetin and kaempferol derivatives which have fluorescence behavior by appropriate treatment, kaempferol and its derivatives are localized exclusively in the cuticle. Results showed that the content of astragalin in Apocyni Veneti Folium from different parts revealed the decreasing trend, while hyperin and isoquercitrin were higher in June and July analyzed by HPLC. In summary, the site-specific accumulation of flavonoids in Apocyni Veneti Folium can be determined by LSCM and vanillin-HCl staining. The contents of flavonoids in Apocyni Veneti Folium are correlated with harvest times and growth parts.

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The Phenols in the Wax and in the Suberin Polymer of Green Cotton Fibres and Their Functions

Cited by 13 publications

References 0 publications

Glycerol Is a Suberin Monomer. New Experimental Evidence for an Old Hypothesis1

Glycerol Is a Suberin Monomer. New Experimental Evidence for an Old Hypothesis1

Seasonal accumulation of ultraviolet‐B screening pigments in needles of Norway spruce (Picea abies (L.) Karst.)

Site‐specific accumulation and dynamic change of flavonoids in Apocyni Veneti Folium

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