The phenomenon of endopolyploidy in some species of the Chenopodioideae (Amaranthaceae)

Skaptsov, M. V.; Lomonosova, Maria N.; Куцев, М. Г.; Смирнов, С. В.; Шмаков, А. И.

doi:10.1080/23818107.2016.1276475

Cited by 6 publications

(3 citation statements)

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“…Trichomes typically are thought to show elevated levels of endopolyploidy, and on average, mature trichomes show a DNA content of between 16C and 32C (De Rocher et al, 1990;Melaragno et al, 1993;Hülskamp, 2004;Skaptsov et al, 2017); however, several Arabidopsis trichome mutants have C values reported up to 64C (Hülskamp, 2004). EBC, which line the leaves, stems, and flower buds of both salt-treated and untreated M. crystallinum plants, are considered large modified balloon-like trichomes (Adams et al, 1998;Oh et al, 2015) and may contribute to the polyploidy measured in the whole leaf.…”

Section: Discussionmentioning

confidence: 99%

“…This is in contrast to true polyploid organisms, such as autopolyploids and allopolyploids, in which the duplicated DNA content is inherited through the germline and perpetuated over subsequent generations in all cells (De Storme and Mason, 2014;Scholes and Paige, 2015). Endopolyploidy is present in animals but is particularly wide-spread among insects and plants (Edgar et al, 2014;Rangel et al, 2015;Leitch and Dodsworth, 2017), with an estimated 70% to 90% of angiosperms presenting endopolyploidy in at least one cell type (Joubès and Chevalier, 2000;Skaptsov et al, 2017). While embryonic, meristematic, and guard cells remain at the 2C DNA content, most other cell types, including those from roots, stems, leaves, flowers, fruits, and seeds, can undergo independent rounds of endoreduplication during development.…”

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Making Epidermal Bladder Cells Bigger: Developmental- and Salinity-Induced Endopolyploidy in a Model Halophyte

et al. 2018

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Endopolyploidy occurs when DNA replication takes place without subsequent mitotic nuclear division, resulting in cell-specific ploidy levels within tissues. In plants, endopolyploidy plays an important role in sustaining growth and development, but only a few studies have demonstrated a role in abiotic stress response. In this study, we investigated the function of ploidy level and nuclear and cell size in leaf expansion throughout development and tracked cell type-specific ploidy in the halophyte In addition to developmental endopolyploidy, we examined the effects of salinity stress on ploidy level. We focused specifically on epidermal bladder cells (EBC), which are modified balloon-like trichomes, due to their large size and role in salt accumulation. Our results demonstrate that ploidy increases as the leaves expand in a similar manner for each leaf type, and ploidy levels up to 512C were recorded for nuclei in EBC of leaves of adult plants. Salt treatment led to a significant increase in ploidy levels in the EBC, and these cells showed spatially related differences in their ploidy and nuclear and cell size depending on the positions on the leaf and stem surface. Transcriptome analysis highlighted salinity-induced changes in genes involved in DNA replication, cell cycle, endoreduplication, and trichome development in EBC. The increase in cell size and ploidy observed in under salinity stress may contribute to salt tolerance by increasing the storage capacity for sodium sequestration brought about by higher metabolic activity driving rapid cell enlargement in the leaf tissue and EBC.

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Section: Discussionmentioning

confidence: 99%

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Making Epidermal Bladder Cells Bigger: Developmental- and Salinity-Induced Endopolyploidy in a Model Halophyte

et al. 2018

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“…Leaf tissue of the studied plant and stan dard was chopped with 500 μl of modified icecold Otto I buffer (Otto 1992) complemented with 0.1 m citric acid plus 0.5 % Triton X100. The nuclear suspension was fil tered in a nylon membrane with a pore size of 42 μm and mixed with a staining solution consisting of 1 ml Tris-MgCl 2 buffer (0.4 m Trisbase, 4 mm MgCl 2 × 6H 2 O) with propidium iodide (50 μg/ml), RNase (50 μg/ml) and βmercaptoethanol (1 μl/ml) (Doležel et al 1998, Pfosser et al 1995, Skaptsov et al 2017. For each sample at least 10 000 nuclei were analyzed.…”

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confidence: 99%

Nuclear genome size analysis in Krascheninnikovia ceratoides s. l. (Amaranthaceae)

Lomonosova,

Pankova,

Korolyuk

et al. 2024

Bot Pac

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The total nuclear DNA amount (2C value) of 75 individuals of Krascheninnikovia ceratoides s. l. was assessed by flow cytometry in 16 populations. The mean 2C value in all samples ranged from 2.59 to 6.99 pg. The relationship between total 2C values and ploidy level was calibrated using chromosome counting in four populations. Diploids (2n = 18) and tetraploids (2n = 36) were revealed. In diploid plants, mean 2C value varied between 2.59 to 3.72 pg, in tetraploids − from 6.04 to 6.99 pg. The GS of diploids is approximately half the GS of tetraploids. Diploids were observed in seven populations from the Orenburg Region and the Republic of Tyva (Russia), Kyzylorda and Almaty Regions (Kazakhstan). Tetraploids were recorded in eight populations from Kazakhstan, Orenburg and Novosibirsk Regions, from the Republics of Altai and Sakha (Yakutia). One mixed population was found in the Republic of Khakassia represented by di- and tetraploids. We did not find any regularity in the geographical distribution of the two cytotypes. The studied plants showed no significant morphological features of taxonomic value, therefore we consider all the samples belong to Krascheninnikovia ceratoides s. str.

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Protocol of European Radish (Raphanus sativus L.) Microspore Culture for Doubled Haploid Plant Production

Kozar

Домблидес

2021

Methods in Molecular Biology

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The phenomenon of endopolyploidy in some species of the Chenopodioideae (Amaranthaceae)

Cited by 6 publications

References 48 publications

Making Epidermal Bladder Cells Bigger: Developmental- and Salinity-Induced Endopolyploidy in a Model Halophyte

Making Epidermal Bladder Cells Bigger: Developmental- and Salinity-Induced Endopolyploidy in a Model Halophyte

Nuclear genome size analysis in Krascheninnikovia ceratoides s. l. (Amaranthaceae)

Protocol of European Radish (Raphanus sativus L.) Microspore Culture for Doubled Haploid Plant Production

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