The phenotype of gingival fibroblasts and their potential use in advanced therapies

García, Sandra Liliana Alfonso; Parada-Sanchez, Monica Tatiana; Arboleda-Toro, David

doi:10.1016/j.ejcb.2020.151123

Cited by 21 publications

(16 citation statements)

References 166 publications

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“…Fibroblasts are abundant in connective tissues, where they regulate tissue development, organogenesis, and homeostasis (Häkkinen et al, 2014; Foote et al, 2019). The main function of fibroblasts is the synthesis of the extracellular matrix (ECM), which provides structural integrity for the tissues (Alfonso García et al, 2020). Furthermore, fibroblasts participate in wound healing and tissue regeneration, immunological regulation, mechano‐transduction, angiogenesis, and organ fibrosis (Haniffa et al, 2009; Barnes et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Fibroblasts of the oral mucosae represent a resource of great interest for regenerative approaches due to their multi‐potency and plasticity, their ease of access by means of minimally invasive procedures that do not generate aesthetic problems, and efficient culturing methods (Zhang et al, 2012; Alfonso García et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, many of the studies comparing fibroblasts within oral mucosa have not clearly defined the anatomic origin of the cells within the oral cavity and/or have not studied parallel cell lines, making comparisons between different fibroblast populations challenging (Häkkinen et al, 2014). Moreover, the literature lacks comprehensive molecular data on the differences between masticatory and lining oral mucosal fibroblasts (Alfonso García et al, 2020). Consequently, it is necessary to characterize more profoundly the populations of fibroblasts present in both lining and masticatory mucosae.…”

Section: Introductionmentioning

confidence: 99%

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Fibroblasts from the oral masticatory and lining mucosa have different gene expression profiles and proliferation rates

Kabakov

Nemcovsky

Plasmanik-Chor

et al. 2021

J Clinic Periodontology

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Aims: To compare the gene expression profiles and proliferation rates of fibroblasts from the oral lining and masticatory mucosae.Materials and methods: Primary human fibroblasts were retrieved from the posterior masticatory hard palate and the lining alveolar mucosa of five individuals. The gene expression profile was evaluated using total RNA sequencing. The proliferation rate was determined colorimetrically.Results: Substantial differences in specific gene groups and pathways were observed between fibroblasts from the two tissues. Significantly enriched gene ontology processes were focused on the extracellular components. Lining mucosa fibroblasts exhibited significantly higher expression of the principal structural collagens, cranial neural crest markers, and homeobox genes associated with positional memory. Masticatory mucosa fibroblasts showed greater expression of genes related to transforming growth factor-β signalling, which may be associated with fibrosis. In addition, they expressed higher levels of the EP 2 prostaglandin E 2 receptor and Toll-like receptor 1. Finally, masticatory mucosa fibroblasts exhibited a 10%-30% higher proliferation rate.Conclusions: Fibroblasts from the lining and masticatory oral mucosae are phenotypically heterogeneous, presenting distinct gene expression profiles and proliferation rates. These features may contribute to their specific physiological functions and have relevance for potential therapeutic applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fibroblasts from the oral masticatory and lining mucosa have different gene expression profiles and proliferation rates

Kabakov

Nemcovsky

Plasmanik-Chor

et al. 2021

J Clinic Periodontology

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“…In addition to this, since the main function of gingival fibroblasts is the synthesis of important components of the ECM, for example collagen, which dynamically interact with the newly formed collagen matrix to generate structural support to the tissue, it is clear that the collagen itself can act as a stimulus for proliferation, differentiation, and activation of these cells [ 25 ]. Various sources and formats of collagen have been proposed for periodontal and peri-implant tissues regeneration.…”

Section: Introductionmentioning

confidence: 99%

Morpho-Functional Effect of a New Collagen-Based Medical Device on Human Gingival Fibroblasts: An In Vitro Study

Romasco

Mandrillo²,

Morsut

et al. 2023

Biomedicines

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Maintaining periodontal and peri-implant soft tissues health is crucial for the long-term health of teeth and dental implants. New biomedical strategies aimed at avoiding connective tissue alterations and related diseases (e.g., periodontitis and peri-implantitis) are constantly evolving. Among these, collagen-based medical products have proven to be safe and effective. Accordingly, the aim of the present study was to evaluate the effects of Dental SKIN BioRegulation (Guna S.p.a., Milan, Italy), a new injectable medical device composed of type I collagen of porcine origin, on primary cultures of human gingival fibroblasts (hGF). To this end, hGF were cultured on collagen-coated (COL, 100 µg/2 mL) or uncoated plates (CTRL) before evaluating cell viability (24 h, 48 h, 72 h, and 7 d), wound healing properties (3 h, 6 h, 12 h, 24 h, and 48 h), and the activation of mechanotransduction markers, such as FAK, YAP, and TAZ (48 h). The results proved a significant increase in cell viability at 48 h (p < 0.05) and wound closure at 24 h (p < 0.001) of hGF grown on COL, with an increasing trend at all time-points. Furthermore, COL significantly induced the expression of FAK and YAP/TAZ (p < 0.05), thereby promoting the activation of mechanotransduction signaling pathways. Overall, these data suggest that COL, acting as a mechanical bio-scaffold, could represent a useful treatment for gingival rejuvenation and may possibly help in the resolution of oral pathologies.

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“…Oral mucosae are an appropriate cell source for regenerative medicine, as they are easily accessed through minimally invasive procedures without generating esthetic problems [ 1 ]. Human gingival fibroblasts (hGFs) are cells with desirable phenotypic characteristics for regenerative strategies of soft and mineralized craniofacial tissues because they can undergo a variety of alternative destinations, such as myofibroblasts, pericytes, endothelial cells, and induced pluripotent stem cells (iPSC), in response to extrinsic mechanical or chemical factors [ 2 ]. Increased cellular plasticity and proliferation rate in favor of hGFs have been also suggested when comparing their gene expression profile with lining mucosa fibroblasts [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural Characterization of Human Gingival Fibroblasts in 3D Culture

García

Uribe

López

et al. 2022

Cells

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Cell spheroids are applied in various fields of research, such as the fabrication of three-dimensional artificial tissues in vitro, disease modeling, stem cell research, regenerative therapy, and biotechnology. A preclinical 3D culture model of primary human gingival fibroblasts free of external factors and/or chemical inducers is presented herein. The ultrastructure of the spheroids was characterized to establish a cellular model for the study of periodontal tissue regeneration. The liquid overlay technique was used with agarose to generate spheroids. Fibroblasts in 2D culture and cell spheroids were characterized by immunofluorescence, and cell spheroids were characterized by optical and scanning electron microscopy, energy-dispersive X-ray spectroscopy, electron backscatter diffraction, and Fourier transform infrared spectroscopy. Osteogenically related genes were analyzed by RT-qPCR. Gingival fibroblasts formed spheroids spontaneously and showed amorphous calcium phosphate nanoparticle deposits on their surface. The results suggest that human gingival fibroblasts have an intrinsic potential to generate a mineralized niche in 3D culture.

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The phenotype of gingival fibroblasts and their potential use in advanced therapies

Cited by 21 publications

References 166 publications

Fibroblasts from the oral masticatory and lining mucosa have different gene expression profiles and proliferation rates

Fibroblasts from the oral masticatory and lining mucosa have different gene expression profiles and proliferation rates

Morpho-Functional Effect of a New Collagen-Based Medical Device on Human Gingival Fibroblasts: An In Vitro Study

Ultrastructural Characterization of Human Gingival Fibroblasts in 3D Culture

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