The phenotypic characterisation of R2 generation transgenic rice plants under field and glasshouse conditions

Lynch, P. T.; Jones, J.; Blackhall, N. W.; Davey, M. R.; Power, J. B.; Cocking, E. C.; Nelson, M. R.; Orum, T. V.; Orth, C. E.; Schuh, W.

doi:10.1007/978-94-011-0357-2_48

Cited by 9 publications

(6 citation statements)

References 15 publications

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“…The variations include stunted plant growth (Lynch et al, 1995;Singh et al, 1998), stem, leaf, and flower abnormalities (Gonsalves et al, 1994), reduced fertility (Lynch et al, 1995;Singh et al, 1998), and polyploidization (Imai et al, 1993;Gonsalves et al, 1994;Singh et al, 1998;Cervera et al, 2000). Similar somaclonal variations were also observed in the present study for transgenic agapanthus plants.…”

Section: Discussionsupporting

confidence: 82%

“…Somaclonal variation has extensively been reported in transgenic plants (Imai et al, 1993;Gonsalves et al, 1994;Lynch et al, 1995;Singh et al, 1998;Cervera et al, 2000;Sala et al, 2000) as well as nontransgenic, tissue-culturederived plants (Larkin and Scowcroft, 1981;Karp, 1995) in different species. The variations include stunted plant growth (Lynch et al, 1995;Singh et al, 1998), stem, leaf, and flower abnormalities (Gonsalves et al, 1994), reduced fertility (Lynch et al, 1995;Singh et al, 1998), and polyploidization (Imai et al, 1993;Gonsalves et al, 1994;Singh et al, 1998;Cervera et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

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Somaclonal variation and stability of GUS gene expression in transgenic agapanthus (Agapanthus praecox ssp. orientalis) plants at the flowering stage

Mori

Oka

Umehara

et al. 2007

In Vitro Cell.Dev.Biol.-Plant

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Agapanthus (Agapanthus praecox ssp. orientalis), a liliaceous perennial, is cultivated as an ornamental plant because of its beautiful, blue-violet to white flowers. We have previously produced transgenic plants containing the ß-glucuronidase (GUS) reporter gene under the control of cauliflower mosaic virus (CaMV) 35S promoter in an experimental strain of Agapanthus via Agrobacteriummediated genetic transformation. These transgenic plants have been cultivated for 5 yr after transplantation to pots. In the present study, we carried out morphological characterization, and examination of the ploidy level, pollen fertility and stability of GUS gene expression in 12 transgenic plants derived from five independent lines at the flowering stage. Flow cytometry (FCM) analysis indicated that transgenic plants of four lines kept the diploid level, but those of the remaining one line were tetraploid. For all of the 12 transgenic plants, some morphological variations were observed both in vegetative and floral organs such as decreased number of leaves per inflorescence, smaller leaves, shorter inflorescence stalks, decreased number of florets per inflorescence and smaller florets. Pollen fertility of all the transgenic plants was below 5% as determined by acetocarmine staining. All the 12 transgenic plants showed stable expression of the GUS gene in leaves, roots, tepals, pistils, and stamens, as indicated by histochemical GUS assay, fluorometric GUS assay, and/or real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis. No apparent GUS gene silencing was observed in transgenic agapanthus plants even after 5 yr of cultivation.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Somaclonal variation and stability of GUS gene expression in transgenic agapanthus (Agapanthus praecox ssp. orientalis) plants at the flowering stage

Mori

Oka

Umehara

et al. 2007

In Vitro Cell.Dev.Biol.-Plant

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“…The transgenic line with lysozyme gene from Bacteriophage T4 had been predicted to only acquire resistance to fungi and bacteria, but variability of agronomic characters, quality characters and mineral element contents was also found in high generation, showing that heritable variability occurred. These variances were probably caused by three factors (Jahne et al, 1995;Labra et al, 2001;Lynch et al, 1995). The first was the introduced foreign gene.…”

Section: Discussionmentioning

confidence: 99%

Potential variability of trans-lysozyme gene rice under ecological conditions of Yunnan Province, China

Chen

Tian

et al. 2008

Front. Biol. China

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Using trans-lysozyme gene rice line D2-1-2 and its restorer Zhonghua No.9 as test materials, we studied their agronomic characters and grain qualities under two ecological conditions of Yunnan Province. The results show that there were no significant differences in the agronomic characters between D2-1-2 and Zhonghua No.9, but the seed setting rate and plant height of D2-1-2 were significantly lower, while the protein, amino acid and mineral element (Ca, Fe and Zn in particular) contents in its crude grain were higher than those of Zhonghua No.9. It is suggested that there could be some potential genetic variances in the transgenic plant and the possibly induced environmental risk should be evaluated in multi-plots for several years.

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“…Variation among traits apparently not related to a foreign gene has also been reported. In transgenic rice (R 2 generation), plants were signifi cantly shorter, fl owered later, and were partially sterile as compared to their nontransgenic controls (Lynch et al, 1995). Morphologically abnormal fl owers that failed to set seeds were observed in soybean [Glycine max (L.) Merr.]…”

Section: Limitations For Direct Adoption Of Transgenic Plantsmentioning

confidence: 99%

Transgenic Breeding: Perspectives and Prospects

et al. 2009

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Transgenic technology serves to introduce gene sequences for expression of a desired trait. Production of transgenic plants is reported in many crops, but commercialization is limited to a few selected crops, such as cotton (Gossypium hirsutum L.), corn (Zea mays L.), soybean [Glycine max (L.) Merr.], and canola (Brassica napus L. and B. rapa L.). This paper presents the sequential processes of transgenic event design, event selection, and “cleaning up” genetic background for forward breeding programs. Expression of the foreign gene cannot be viewed in isolation and is more complex than has been assumed because of the interaction of transgene with native genes. Variations among clones and within the progeny are observed, and hence all the clones are taken into account for evaluation. Plant breeding must be involved to move transgenes from transformable but agronomically unacceptable genotypes into elite breeding lines with two backcrosses. Production of transgenic plants in large numbers is difficult and laborious and requires large investments. It is worthwhile investing in parallel efforts to incorporate the transgene into improved plant material to satisfy commercial interests.

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The phenotypic characterisation of R2 generation transgenic rice plants under field and glasshouse conditions

Cited by 9 publications

References 15 publications

Somaclonal variation and stability of GUS gene expression in transgenic agapanthus (Agapanthus praecox ssp. orientalis) plants at the flowering stage

Somaclonal variation and stability of GUS gene expression in transgenic agapanthus (Agapanthus praecox ssp. orientalis) plants at the flowering stage

Potential variability of trans-lysozyme gene rice under ecological conditions of Yunnan Province, China

Transgenic Breeding: Perspectives and Prospects

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