The phenylcoumaran benzylic ether reductase gene PtPCBER improves the salt tolerance of transgenic poplar through lignan-mediated reactive oxygen species scavenging

Wei, Mingxing; Yu, Chunyan; Ge, Bingkun; Liu, Yue; Zhang, Haiyang; Duan, Chunli; Zhang, Juan; Mao, Tingting; Huang, Huiqing; Xie, Yinshuai; Gao, Hongsheng; Zhang, Hongxia; Wang, Aike; Yi, Yan-Jun

doi:10.1016/j.envexpbot.2022.104946

Cited by 6 publications

(3 citation statements)

References 49 publications

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“…Soil salt-alkaline stress enhanced the metabolites accumulation of amino acids, alkaloids, organic acids, and polyphenols contents in wolfberry fruits [21]. Furthermore, Wei et al demonstrated that the overexpression of the phenylcoumaran benzylic ether reductase gene (PtPCBER) in the phenylpropane metabolic pathway improved the salt tolerance of poplar through lignan-mediated reactive oxygen species clearance [57]. Studies of mungbean reported that the contents of phenylpropanoid-derived metabolites, including catechin, chlorogenic acid, isovitexin, p-coumaric acid, syringic acid, ferulic acid, and vitexin, significantly increased under salinity conditions [58].…”

Section: Discussionmentioning

confidence: 99%

Integrated Analysis of Transcriptome and Metabolome Reveals Molecular Mechanisms of Rice with Different Salinity Tolerances

Zhou,

Liu,

Meng

et al. 2023

Plants

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Rice is a crucial global food crop, but it lacks a natural tolerance to high salt levels, resulting in significant yield reductions. To gain a comprehensive understanding of the molecular mechanisms underlying rice’s salt tolerance, further research is required. In this study, the transcriptomic and metabolomic differences between the salt-tolerant rice variety Lianjian5 (TLJIAN) and the salt-sensitive rice variety Huajing5 (HJING) were examined. Transcriptome analysis revealed 1518 differentially expressed genes (DEGs), including 46 previously reported salt-tolerance-related genes. Notably, most of the differentially expressed transcription factors, such as NAC, WRKY, MYB, and EREBP, were upregulated in the salt-tolerant rice. Metabolome analysis identified 42 differentially accumulated metabolites (DAMs) that were upregulated in TLJIAN, including flavonoids, pyrocatechol, lignans, lipids, and trehalose-6-phosphate, whereas the majority of organic acids were downregulated in TLJIAN. The interaction network of 29 differentially expressed transporter genes and 19 upregulated metabolites showed a positive correlation between the upregulated calcium/cation exchange protein genes (OsCCX2 and CCX5_Ath) and ABC transporter gene AB2E_Ath with multiple upregulated DAMs in the salt-tolerant rice variety. Similarly, in the interaction network of differentially expressed transcription factors and 19 upregulated metabolites in TLJIAN, 6 NACs, 13 AP2/ERFs, and the upregulated WRKY transcription factors were positively correlated with 3 flavonoids, 3 lignans, and the lipid oleamide. These results suggested that the combined effects of differentially expressed transcription factors, transporter genes, and DAMs contribute to the enhancement of salt tolerance in TLJIAN. Moreover, this study provides a valuable gene–metabolite network reference for understanding the salt tolerance mechanism in rice.

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Section: Discussionmentioning

confidence: 99%

Integrated Analysis of Transcriptome and Metabolome Reveals Molecular Mechanisms of Rice with Different Salinity Tolerances

Zhou,

Liu,

Meng

et al. 2023

Plants

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“…Casparian strip formation may increase the blocking or reduction in heavy metal transport. In addition to being a reserve precursor for lignin formation, lignan is also a secondary metabolite that may be involved in the regulation of oxidative stress and flavonoid production [263][264][265]. Reduced lignan has been shown to strongly mediate ROS scavenging in the xylem, serving a strong protective function for the vasculature [264,266].…”

Section: Analysis Of the Top Downregulated Genes For Copper-resistant...mentioning

confidence: 99%

Decrypting Molecular Mechanisms Involved in Counteracting Copper and Nickel Toxicity in Jack Pine (Pinus banksiana) Based on Transcriptomic Analysis

Moy,

Nkongolo

2024

Plants

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The remediation of copper and nickel-afflicted sites is challenged by the different physiological effects imposed by each metal on a given plant system. Pinus banksiana is resilient against copper and nickel, providing an opportunity to build a valuable resource to investigate the responding gene expression toward each metal. The objectives of this study were to (1) extend the analysis of the Pinus banksiana transcriptome exposed to nickel and copper, (2) assess the differential gene expression in nickel-resistant compared to copper-resistant genotypes, and (3) identify mechanisms specific to each metal. The Illumina platform was used to sequence RNA that was extracted from seedlings treated with each of the metals. There were 449 differentially expressed genes (DEGs) between copper-resistant genotypes (RGs) and nickel-resistant genotypes (RGs) at a high stringency cut-off, indicating a distinct pattern of gene expression toward each metal. For biological processes, 19.8% of DEGs were associated with the DNA metabolic process, followed by the response to stress (13.15%) and the response to chemicals (8.59%). For metabolic function, 27.9% of DEGs were associated with nuclease activity, followed by nucleotide binding (27.64%) and kinase activity (10.16%). Overall, 21.49% of DEGs were localized to the plasma membrane, followed by the cytosol (16.26%) and chloroplast (12.43%). Annotation of the top upregulated genes in copper RG compared to nickel RG identified genes and mechanisms that were specific to copper and not to nickel. NtPDR, AtHIPP10, and YSL1 were identified as genes associated with copper resistance. Various genes related to cell wall metabolism were identified, and they included genes encoding for HCT, CslE6, MPG, and polygalacturonase. Annotation of the top downregulated genes in copper RG compared to nickel RG revealed genes and mechanisms that were specific to nickel and not copper. Various regulatory and signaling-related genes associated with the stress response were identified. They included UGT, TIFY, ACC, dirigent protein, peroxidase, and glyoxyalase I. Additional research is needed to determine the specific functions of signaling and stress response mechanisms in nickel-resistant plants.

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“…The LbDREBtransgenic Populus ussuriensis (incorporating a DREB gene from the halophyte Limonium bicolor) exhibited enhanced salt resistance by increasing its reactive oxygen species (ROS), reducing its malondialdehyde (MDA) content, and increasing its proline accumulation in leaves (Zhao et al, 2018). The overexpression of PtPCBER promoted salt tolerance in transgenic poplar by increasing ROS scavenging capacity (Wei et al, 2022).…”

Section: Salinity Stressmentioning

confidence: 99%

Developing tree improvement strategies for challenging environmental stresses under global climate change: a review from traditional tree breeding to genomics of adaptive traits for the quaking aspen

Mu,

Ding,

Chen

et al. 2023

Preprint

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Quaking or trembling aspen in North America and Euro-Asia(Populus tremuloidesandP. tremula,respectively) are both widely distributed species with a long history of scientific research and tree improvement work in areas such as carbon sequestration, biomass, bioenergy, wood, and fiber, as well as studies evaluating the social, economic, and ecological benefits of the species. This chapter reviews the ecological genetics and genomics of quaking aspen’s adaptive traits with a broad perspective of the relationship between phenotypic variation and genetic (G) and environmental (E) effects as well as their interactions (GxE). Based on recent studies, several adaptive traits are discussed, including spring and fall phenology and stress tolerance to environmental factors such as frost, salinity, drought, heat, UV radiation, etc. We also conducted a meta-analysis of empirical studies on adaptive traits ofP. tremuloidesand its sister species, as research usingP. tremuloidesas a true “model species” is currently limited. However, molecular tools and experimental designs in the form of different common gardens constitute an integrated pathway for the development of traits and varieties/populations to promote reforestation under changing climatic conditions.

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The phenylcoumaran benzylic ether reductase gene PtPCBER improves the salt tolerance of transgenic poplar through lignan-mediated reactive oxygen species scavenging

Cited by 6 publications

References 49 publications

Integrated Analysis of Transcriptome and Metabolome Reveals Molecular Mechanisms of Rice with Different Salinity Tolerances

Integrated Analysis of Transcriptome and Metabolome Reveals Molecular Mechanisms of Rice with Different Salinity Tolerances

Decrypting Molecular Mechanisms Involved in Counteracting Copper and Nickel Toxicity in Jack Pine (Pinus banksiana) Based on Transcriptomic Analysis

Developing tree improvement strategies for challenging environmental stresses under global climate change: a review from traditional tree breeding to genomics of adaptive traits for the quaking aspen

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