The Mobile Asteroid Surface Scout (MASCOT), an Asteroid Lander carried by the Hayabusa2 spacecraft, successfully landed on the Near-Earth Asteroid (162173) Ryugu on October 03, 2018. Thereby accomplishing the first-ever landing of a European spacecraft on the surface of this type of celestial body. MASCOT was a prototype design of a new class of nano-size surface science packages for the exploration of small solar system bodies. The very low gravity (thus, very low escape velocity) of the target body required the design of a miniaturized deployment mechanism with a relatively small, well-reproducible separation velocity. In addition, the mechanism also had to safely restrain the lander to the mother spacecraft during the launch and its 3.5-year cruise phase. In this paper, we describe in detail the design, numerical analysis and test of this newly developed separation mechanism. Furthermore, we compare the mechanism to other existing deployment systems and verify its performance with two independent analysis methods using actual flight data taken during the ultimate flight activation event, which initiated the successful delivery and surface operation of the MASCOT asteroid lander.