The phosphoinositide dependent-phospholipase C pathway differentially controls the basal expression of<i>DREB1</i>and<i>DREB2</i>genes

Ruelland, Éric; Djafi, Nabila; Zachowski, Alain

doi:10.4161/psb.26895

Cited by 7 publications

(8 citation statements)

References 10 publications

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“…Glycerophospholipids like phosphatidic acid (PA) (FC = 17.72), phosphatidylcholine (FC = 6.56), phosphatidylinositol (FC = 5.77) are known to be deposited to enforce cell walls. Furthermore, these are also known to perceive and transmit signals activating downstream genes eventually regulating R genes to biosynthesize RR metabolites and RR proteins [ 43 ]. These compounds are either converted into bioactive lipids (components of lipid bilayer of cell membrane) or stay as soluble molecules (messengers), further binding to the downstream mitogen activated protein kinases (MAPK), further affecting the enzymatic activities, vesicle trafficking and ion fluxes [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Integrated Metabolo-Transcriptomics Reveals Fusarium Head Blight Candidate Resistance Genes in Wheat QTL-Fhb2

et al. 2016

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BackgroundFusarium head blight (FHB) caused by Fusarium graminearum not only causes severe losses in yield, but also reduces quality of wheat grain by accumulating mycotoxins. Breeding for host plant resistance is considered as the best strategy to manage FHB. Resistance in wheat to FHB is quantitative in nature, involving cumulative effects of many genes governing resistance. The poor understanding of genetics and lack of precise phenotyping has hindered the development of FHB resistant cultivars. Though more than 100 QTLs imparting FHB resistance have been reported, none discovered the specific genes localized within the QTL region, nor the underlying mechanisms of resistance.FindingsIn our study recombinant inbred lines (RILs) carrying resistant (R-RIL) and susceptible (S-RIL) alleles of QTL-Fhb2 were subjected to metabolome and transcriptome profiling to discover the candidate genes. Metabolome profiling detected a higher abundance of metabolites belonging to phenylpropanoid, lignin, glycerophospholipid, flavonoid, fatty acid, and terpenoid biosynthetic pathways in R-RIL than in S-RIL. Transcriptome analysis revealed up-regulation of several receptor kinases, transcription factors, signaling, mycotoxin detoxification and resistance related genes. The dissection of QTL-Fhb2 using flanking marker sequences, integrating metabolomic and transcriptomic datasets, identified 4-Coumarate: CoA ligase (4CL), callose synthase (CS), basic Helix Loop Helix (bHLH041) transcription factor, glutathione S-transferase (GST), ABC transporter-4 (ABC4) and cinnamyl alcohol dehydrogenase (CAD) as putative resistance genes localized within the QTL-Fhb2 region.ConclusionSome of the identified genes within the QTL region are associated with structural resistance through cell wall reinforcement, reducing the spread of pathogen through rachis within a spike and few other genes that detoxify DON, the virulence factor, thus eventually reducing disease severity. In conclusion, we report that the wheat resistance QTL-Fhb2 is associated with high rachis resistance through additive resistance effects of genes, based on cell wall enforcement and detoxification of DON. Following further functional characterization and validation, these resistance genes can be used to replace the genes in susceptible commercial cultivars, if nonfunctional, based on genome editing to improve FHB resistance.

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Section: Discussionmentioning

confidence: 99%

Integrated Metabolo-Transcriptomics Reveals Fusarium Head Blight Candidate Resistance Genes in Wheat QTL-Fhb2

et al. 2016

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“…There is a special and thoroughly studied subgroup, IIIc, namely the DREB1s. The AtDREB1 factors are mostly induced in response to chilling [ 88 ]. Similar molecular mechanisms and physiological responses, induced and regulated by DREB1 genes, appear to occur in monocots [ 5 , 25 , 65 , 89 ].…”

Section: Discussionmentioning

confidence: 99%

“…Some subgroup IIId genes may have constitutive expression or participate in responses to abiotic stress, for example, OsDREB4-2 was induced by drought and salt stresses, but OsDREB4-1 was constitutively expressed [ 90 ]. The group IV genes were designated as DREB2 genes, although they were reported as mostly involved in drought and heat responses [ 88 ]; however, they may have more functions, such as DREB2A being involved in drought-, salt- and heat-stress responses in Arabidopsis [ 91 ]. AtDREB2C -overexpressing Arabidopsis plants were dehydration sensitive, and were freezing and heat tolerant [ 92 ].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of the AP2/ERF Transcription Factors Family and the Expression Patterns of DREB Genes in Moso Bamboo (Phyllostachys edulis)

Wu¹,

Lv²,

Li³

et al. 2015

PLoS ONE

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The AP2/ERF transcription factor family, one of the largest families unique to plants, performs a significant role in terms of regulation of growth and development, and responses to biotic and abiotic stresses. Moso bamboo (Phyllostachys edulis) is a fast-growing non-timber forest species with the highest ecological, economic and social values of all bamboos in Asia. The draft genome of moso bamboo and the available genomes of other plants provide great opportunities to research global information on the AP2/ERF family in moso bamboo. In total, 116 AP2/ERF transcription factors were identified in moso bamboo. The phylogeny analyses indicated that the 116 AP2/ERF genes could be divided into three subfamilies: AP2, RAV and ERF; and the ERF subfamily genes were divided into 11 groups. The gene structures, exons/introns and conserved motifs of the PeAP2/ERF genes were analyzed. Analysis of the evolutionary patterns and divergence showed the PeAP2/ERF genes underwent a large-scale event around 15 million years ago (MYA) and the division time of AP2/ERF family genes between rice and moso bamboo was 15–23 MYA. We surveyed the putative promoter regions of the PeDREBs and showed that largely stress-related cis-elements existed in these genes. Further analysis of expression patterns of PeDREBs revealed that the most were strongly induced by drought, low-temperature and/or high salinity stresses in roots and, in contrast, most PeDREB genes had negative functions in leaves under the same respective stresses. In this study there were two main interesting points: there were fewer members of the PeDREB subfamily in moso bamboo than in other plants and there were differences in DREB gene expression profiles between leaves and roots triggered in response to abiotic stress. The information produced from this study may be valuable in overcoming challenges in cultivating moso bamboo.

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“…Two of these, phospholipid‐remodeling into galactolipids through the generation of phosphatidic acid (PA) by phospholipase C (first route) or phospholipase D (second route), have been extensively studied in connection to phosphate deficiency responses (Gaude, Nakamura, Scheible, Ohta, & Dörmann, ; Nakamura et al., ). We do not find either of them to respond to increased irradiance, although they are known to repress expression of the transcription factor DREB2A (Figure ; (Ruelland, Djafi, & Zachowski, )). The activation of the GDPD pathway (third route), as a result of Pi limitation, probably outcompetes the PLC pathway, leading to reduced PLC‐mediated repression of DREB2A .…”

Section: Discussionmentioning

confidence: 70%

Photosynthetic response to increased irradiance correlates to variation in transcriptional response of lipid‐remodeling and heat‐shock genes

2018

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Plants have evolved several mechanisms for sensing increased irradiance, involving signal perception by photoreceptors (cryptochromes), and subsequent biochemical (reactive oxygen species, ROS) and metabolic clues to transmit the signals. This results in the increased expression of heat-shock response genes and of the transcription factor LONG HYPOCOTYL 5 (HY5, mediated by the cryptochrome photoreceptor 1, CRY1).Here, we show the existence of another response pathway in Arabidopsis. This pathway evokes the SPX1-mediated expression activation of the transcription factor PHR1 and leads to the expression of several galactolipid biosynthesis genes. Gene expression analysis of accessions Col-0, Ga-0, and Ts-1, showed activated expression of the SPX1/PHR1-mediated gene expression activation pathway acting on galactolipids biosynthesis genes in both Ga-0 and Col-0, but not in Ts-1. The activation of the SPX1/PHR1-mediated response pathway can be associated with lower photosynthesis efficiency in Ts-1, compared to Col-0 and Ga-0. Besides the accession-associated activation of the SPX1/PHR1-mediated response pathway, comparing gene expression in the accessions showed stronger activation of several heat responsive genes in Ga-0, and the opposite in Ts-1, when compared to Col-0, in line with the differences in their efficiency of photosynthesis. We conclude that natural variation in activation of both heat responsive genes and of galactolipids biosynthesis genes contribute to the variation in photosynthesis efficiency in response to irradiance increase. K E Y W O R D SArabidopsis thaliana, high light stress, microarray, natural genetic variation, photosynthesis efficiency, photosystem II

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The phosphoinositide dependent-phospholipase C pathway differentially controls the basal expression ofDREB1andDREB2genes

Cited by 7 publications

References 10 publications

Integrated Metabolo-Transcriptomics Reveals Fusarium Head Blight Candidate Resistance Genes in Wheat QTL-Fhb2

Integrated Metabolo-Transcriptomics Reveals Fusarium Head Blight Candidate Resistance Genes in Wheat QTL-Fhb2

Genome-Wide Analysis of the AP2/ERF Transcription Factors Family and the Expression Patterns of DREB Genes in Moso Bamboo (Phyllostachys edulis)

Photosynthetic response to increased irradiance correlates to variation in transcriptional response of lipid‐remodeling and heat‐shock genes

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