The phospholipase A<sub>2</sub>enzyme complex PAFAH Ib mediates endosomal membrane tubule formation and trafficking

Bechler, Marie E.; Doody, Anne M.; Ha, Kevin D.; Judson, Bret L.; Chen, Ina; Brown, William J.

doi:10.1091/mbc.e09-12-1064

Cited by 17 publications

(30 citation statements)

References 62 publications

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“…Our findings are similar to those of recent studies that demonstrated a nearly identical involvement of other PLA 2 enzymes in membrane tabulation, which is needed for intra-Golgi transport (San Pietro et al, 2009), protein export from the TGN (Bechler et al, 2010) and recycling of endocytosed transferrin (Bechler et al, 2011). The notion that distinct PLA 2 enzymes were found to support non-overlapping trafficking steps suggests that each PLA 2 isoform possesses an array of targeting signals for specific compartmentalization.…”

Section: Discussionsupporting

confidence: 90%

“…The formation of tubular membrane structures operating in several intracellular transport routes has been reported to require catalytic activity of PLA 2 enzymes (Bechler et al, 2011;Bechler et al, 2010;San Pietro et al, 2009). It has been hypothesized that phospholipid hydrolysis by PLA 2 enzymes results in an increase in wedgeshaped lysolipids that induce or support a high curvature of the tubule membrane (Bechler et al, 2012;Ha et al, 2012;Polishchuk et al, 2009).…”

Section: Resultsmentioning

confidence: 99%

“…Over the past few years, two cytosolic PLA 2 enzymes have been reported to regulate the formation of tubular elements that operate in different transport steps. Cytosolic Ca 2+ -dependent PLA 2 a (cPLA 2 a, also known as PLA2G4A) operates in intra-Golgi trafficking (San Pietro et al, 2009), whereas cytoplasmic PLA 2 enzyme complex platelet-activating factor acetylhydrolase (PAFAH) Ib regulates protein export from the Golgi at the TGN level (Bechler et al, 2010) and the distribution and function of endosomes (Bechler et al, 2011). Therefore, it is likely that different PLA 2 enzymes operate in various specific tubule-mediated transport steps.…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, PLA 2 activity has been demonstrated to support endosome tubulation and recycling of endocytosed material back to the cell surface (Bechler et al, 2011;de Figueiredo et al, 2001;Doody et al, 2009). Moreover, identification of several novel PLA 2 isoforms revealed their association with the endo/lysosomal system (Ghosh et al, 2006a;Ohto et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Cytosolic phospholipase A2ε drives recycling in the clathrin-independent endocytic route

Capestrano

Mariggiò

Perinetti

et al. 2014

Journal of Cell Science

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Previous studies have demonstrated that membrane tubulemediated transport events in biosynthetic and endocytic routes require phospholipase A 2 (PLA 2 ) activity. Here, we show that cytosolic phospholipase A 2 e (cPLA 2 e, also known as PLA2G4E) is targeted to the membrane compartments of the clathrinindependent endocytic route through a C-terminal stretch of positively charged amino acids, which allows the enzyme to interact with phosphoinositide lipids [especially PI(4,5)P 2 ] that are enriched in clathrin-independent endosomes. Ablation of cPLA 2 e suppressed the formation of tubular elements that carry internalized clathrin-independent cargoes, such as MHC-I, CD147 and CD55, back to the cell surface and, therefore, caused their intracellular retention. The ability of cPLA 2 e to support recycling through tubule formation relies on the catalytic activity of the enzyme, because the inactive cPLA 2 e S420A mutant was not able to recover either tubule growth or transport from clathrin-independent endosomes. Taken together, our findings indicate that cPLA 2 e is a new important regulator of trafficking processes within the clathrin-independent endocytic and recycling route. The affinity of cPLA 2 e for this pathway supports a new hypothesis that different PLA 2 enzymes use selective targeting mechanisms to regulate tubule formation locally during specific trafficking steps in the secretory and/or endocytic systems.

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Section: Discussionsupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cytosolic phospholipase A2ε drives recycling in the clathrin-independent endocytic route

Capestrano

Mariggiò

Perinetti

et al. 2014

Journal of Cell Science

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“…The role of LIS1 in targeting dynein to membranes is not entirely clear. LIS1 is also a component of the phospholipase platelet-activating factor acetyl hydrolase 1b (PAFAH1B), which modulates membrane structure and dynamics (Bechler et al, 2010;Bechler et al, 2011). It is probable that phospholipid remodelling and motor function are both involved in LIS1-dependent membrane dynamics.…”

Section: Indirect Coupling Of Motors To Membrane Cargomentioning

confidence: 99%

Functional coupling of microtubules to membranes – implications for membrane structure and dynamics

Stephens

2012

Journal of Cell Science

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SummaryThe microtubule network dictates much of the spatial patterning of the cytoplasm, and the coupling of microtubules to membranes controls the structure and positioning of organelles and directs membrane trafficking between them. The connection between membranes and the microtubule cytoskeleton, and the way in which organelles are shaped and moved by interactions with the cytoskeleton, have been studied intensively in recent years. In particular, recent work has expanded our thinking of this topic to include the mechanisms by which membranes are shaped and how cargo is selected for trafficking as a result of coupling to the cytoskeleton. In this Commentary, I will discuss the molecular basis for membrane-motor coupling and the physiological outcomes of this coupling, including the way in which microtubule-based motors affect membrane structure, cargo sorting and vectorial trafficking between organelles. Whereas many core concepts of these processes are now well understood, key questions remain about how the coupling of motors to membranes is established and controlled, about the regulation of cargo and/or motor loading and about the control of directionality.

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Ndel1, Nudel ( Noodle): Flexible in the cell?

et al. 2011

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Nuclear distribution element‐like 1 (Ndel1 or Nudel) was firstly described as a regulator of the cytoskeleton in microtubule and intermediate filament dynamics and microtubule‐based transport. Emerging evidence indicates that Ndel1 also serves as a docking platform for signaling proteins and modulates enzymatic activities (kinase, ATPase, oligopeptidase, GTPase). Through these structural and signaling functions, Ndel1 plays a role in diverse cellular processes (e.g., mitosis, neurogenesis, neurite outgrowth, and neuronal migration). Furthermore, Ndel1 is linked to the etiology of various mental illnesses and neurodegenerative disorders. In the present review, we summarize the physiological and pathological functions associated with Ndel1. We further advance the concept that Ndel1 interfaces GTPases‐mediated processes (endocytosis, vesicles morphogenesis/signaling) and cytoskeletal dynamics to impact cell signaling and behaviors. This putative mechanism may affect cellular functionalities and may contribute to shed light into the causes of devastating human diseases. © 2011 Wiley Periodicals, Inc.

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The phospholipase A₂enzyme complex PAFAH Ib mediates endosomal membrane tubule formation and trafficking

Abstract: For the first time, a cytoplasmic phospholipase A2 enzyme, platelet-activating factor acetylhydrolase (I)b, is described that is directly involved in the formation of membrane tubules from endosomes and trafficking through the endocytic recycling pathway.

Cited by 17 publications

References 62 publications

Cytosolic phospholipase A2ε drives recycling in the clathrin-independent endocytic route

Cytosolic phospholipase A2ε drives recycling in the clathrin-independent endocytic route

Functional coupling of microtubules to membranes – implications for membrane structure and dynamics

Ndel1, Nudel ( Noodle): Flexible in the cell?

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The phospholipase A2enzyme complex PAFAH Ib mediates endosomal membrane tubule formation and trafficking

Abstract: For the first time, a cytoplasmic phospholipase A2 enzyme, platelet-activating factor acetylhydrolase (I)b, is described that is directly involved in the formation of membrane tubules from endosomes and trafficking through the endocytic recycling pathway.

Cited by 17 publications

References 62 publications

Cytosolic phospholipase A2ε drives recycling in the clathrin-independent endocytic route

Cytosolic phospholipase A2ε drives recycling in the clathrin-independent endocytic route

Functional coupling of microtubules to membranes – implications for membrane structure and dynamics

Ndel1, Nudel ( Noodle): Flexible in the cell?

Contact Info

Product

Resources

About

The phospholipase A₂enzyme complex PAFAH Ib mediates endosomal membrane tubule formation and trafficking