The Phosphoproteomic Response of Rice Seedlings to Cadmium Stress

Abstract

The environmental damage caused by cadmium (Cd) pollution is of increasing concern in China. While the overall plant response to Cd has been investigated in some depth, the contribution (if any) of protein phosphorylation to the detoxification of Cd and the expression of tolerance is uncertain. Here, the molecular basis of the plant response has been explored in hydroponically raised rice seedlings exposed to 10 μΜ and 100 μΜ Cd2+ stress. An analysis of the seedlings’ quantitative phosphoproteome identified 2454 phosphosites, associated with 1244 proteins. A total of 482 of these proteins became differentially phosphorylated as a result of exposure to Cd stress; the number of proteins affected in this way was six times greater in the 100 μΜ Cd2+ treatment than in the 10 μΜ treatment. A functional analysis of the differentially phosphorylated proteins implied that a significant number was involved in signaling, in stress tolerance and in the neutralization of reactive oxygen species, while there was also a marked representation of transcription factors.

Phosphoproteomic Analysis of Two Contrasting Maize Inbred Lines Provides Insights into the Mechanism of Salt-Stress Tolerance

“…It can identify thousands of proteins and their modifications in a single experiment [13]. It has been widely used in proteomic and phosphoproteomic analysis on Arabidopsis [14,15,16,17], maize [18,19,20,21], rice [22,23,24], soybean [25,26,27], and sorghum [28,29]. A comparative phosphoproteomic and proteomic analysis on roots of soybean seedlings identified 2692 phosphoproteins and 5509 phosphorylation sites.…”

Genome-wide association studies of ionomic and agronomic traits in USDA mini core collection of rice and comparative analyses of different mapping methods

“…CAL1 was annotated as a defensin-like protein, which could regulate Cd accumulation of rice leaves through translocating Cd from cytosol into extracellular spaces, but not rice grains [14]. We then further analyzed the genes around the QTLs and found there is an ABC transporter (Os01g0121700), its phosphorylation level was up-regulated under high Cd treatment (100 μM CdCl 2 •2.5H 2 O) and it has been shown that the transporter reduces the concentration of Cd 2+ through transporting PCs-Cd into vacuole [79]. Another QTL (Chr6: 29,733,715) was also showed strongly related to Cd concentration in rice grain.…”

Genome-wide association studies of ionomic and agronomic traits in USDA mini core collection of rice and comparative analyses of different mapping methods

“…CAL1 was annotated as a defensin-like protein, which could regulate Cd accumulation of rice leaves through translocating Cd from cytosol into extracellular spaces, but not rice grains [14]. We then further analyzed the genes around the QTLs and found there is an ABC transporter (Os01g0121700), its phosphorylation level was up-regulated under high Cd treatment (100 μM CdCl 2 •2.5H 2 O) and it has been shown that the transporter reduces the concentration of Cd 2+ through transporting PCs-Cd into vacuole [81]. Another QTL (Chr6: 29,733,715) is also showed strongly related to Cd concentration in rice grain.…”