The photo comet assay—A fast screening assay for the determination of photogenotoxicity in vitro

Struwe, Melanie; Greulich, Karl-Otto; Suter, Willi; Plappert‐Helbig, Ulla

doi:10.1016/j.mrgentox.2007.04.014

Cited by 54 publications

(39 citation statements)

References 55 publications

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“…The genotoxicity of fine TiO 2 -or TiO 2 whose particle size has not been defined-has been assessed in several earlier studies (Struwe et al, 2007;Türkez and Geyikoğlu, 2007;Türkez, 2008), but the information on the genotoxicity of nanosized TiO 2 is still scanty. In fact, mammalian and in vitro studies have raised concerns about the toxicity of titanium dioxide nanoparticles, but there are very limited data on ecotoxicity to aquatic life with only a few reports on invertebrates, but almost no information on the toxic effects of TiO 2 NPs in fish (Lovern and Klaper, 2006;Zhang et al, 2007;Handy and Shaw, 2007;Ferin and Oberdörster, 1985;Long et al, 2006;Rothen-Rutishauser et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Genotoxicity assessment of TiO2 nanoparticles in the teleost Danio rerio

Rocco

Santonastaso

Mottola

et al. 2015

Ecotoxicology and Environmental Safety

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Section: Discussionmentioning

confidence: 99%

Genotoxicity assessment of TiO2 nanoparticles in the teleost Danio rerio

Rocco

Santonastaso

Mottola

et al. 2015

Ecotoxicology and Environmental Safety

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“…The sensitivity of the study protocol was confirmed by positive results with 8-methoxypsoralen, acridine, chloropromazine, dacarbazine, ketoprofen and lomefloxacin. Promazine, chlorohexidine, anatase and octylmethoxycinnamate yielded negative results (Struwe et al 2007). The relatively weak cytotoxicity suggests that the doses used in the study were too low.…”

Section: Mammalian Cellsmentioning

confidence: 99%

Titanium dioxide (respirable fraction) [MAK Value Documentation, 2009]

2014

The MAK‐Collection for Occupational Health and Safety

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Published in the series Occupational Toxicants , 2014 The article contains sections titled: Toxic Effects and Mode of Action Mechanism of Action Toxicokinetics and Metabolism Absorption, distribution, elimination Metabolism Effects in Humans Single exposures Repeated exposure Local effects on skin and mucous membranes Allergenic effects Reproductive toxicity Genotoxicity Carcinogenicity Animal Experiments and in vitro Studies Acute toxicity Subacute, subchronic and chronic toxicity Local effects on skin and mucous membranes Allergenic effects Reproductive toxicity Genotoxicity Carcinogenicity Other effects Manifesto (MAK value/characterization)

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“…Irradiation with UV light is performed either with a conventional UV lamp [9,[15][16][17][18] or with a laser microbeam [2][3][4][5][6][7][8]. In short, to generate a laser microbeam, typically a nitrogen laser (337 nm) with nanosecond pulses is coupled via the fluorescence illumination path into a microscope.…”

Section: Microirradiationmentioning

confidence: 99%

“…Therefore, the comet assay has been developed further into a photogenotoxicity assay [16][17][18]. Using this assay, it was shown that sparfloxacin, a substance closely related to levofloxacin (trade name TAVANIC), does not induce DNA damage.…”

Section: Photons May Induce Toxicity Of Drugsmentioning

confidence: 99%

“…Recently, the combination of microirradiation with "Single Cell Gel Electrophoresis" (comet assay) [11][12][13][14], a technique which allows a direct view on DNA damages, has emerged into a tool for studying DNA damage. It has turned out to be a useful tool in nutritional toxicology [15] as well as in studies of toxicity of pharmacologically relevant substances [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

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Photons bring light into DNA repair: The comet assay and laser microbeams for studying photogenotoxicity of drugs and ageing

Greulich

2010

Journal of Biophotonics

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This contribution reviews recent applications of micromanipulation, by UV photons, in DNA repair and ageing research as well as in the evaluation of the phototoxicity of drugs. In some cases, micromanipulation is combined with the comet assay, a technique, which allows a direct view on DNA damages. It is shown that, in humans, the sensitivity of DNA to UV induced damage and its subsequent repair is surprisingly stable up to high age and that drugs which are usually non-toxic induce DNA damage when irradiated in parallel by UV irradiation. Using the immune fluorescent comet assay, IFCA, a variant of the comet assay, direct comparison of the effects of ionizing (137) Cs radiation with those of localized UV radiation is possible. When a laser microbeam is used to damage DNA in a cell nucleus with high temporal and spatial resolution, it can be observed directly how repair molecules accumulate (are recruited) at the site of damage. Comparison of the recruitment speed allows establishing an order of DNA repair events.

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The photo comet assay—A fast screening assay for the determination of photogenotoxicity in vitro

Cited by 54 publications

References 55 publications

Genotoxicity assessment of TiO2 nanoparticles in the teleost Danio rerio

Genotoxicity assessment of TiO2 nanoparticles in the teleost Danio rerio

Titanium dioxide (respirable fraction) [MAK Value Documentation, 2009]

Photons bring light into DNA repair: The comet assay and laser microbeams for studying photogenotoxicity of drugs and ageing

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