The photogenotoxicity of titanium dioxide particles

Nakagawa, Yukari; Wakuri, Shinobu; Sakamoto, Kyoko; Tanaka, Noriho

doi:10.1016/s1383-5718(97)00126-5

Cited by 183 publications

(128 citation statements)

References 16 publications

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“…Strand breaks in DNA were assayed on agarose gels by transformation of a supercoiled plasmid into the relaxed or linear form [14] . Nakagawa et al [12] studied the effects of four sizes of UV-irradiated TiO2 particles on a mouse lymphoma cell line. DNA tail length was measured by SCG assay.…”

Section: Discussionmentioning

confidence: 99%

“…As hole and electron separate successfully on the surface of nanoparticles, they could react with absorbed water or other adsorbates to produce reactive oxygen specimens such as OH• and H2O2 [6] . These reactive radicals can destroy cell membrane structure [11,12] and induce micronuclei (MN) [13] , even cause DNA damage [12,14] . Apoptotic cells can be characterized by a number of morphologic, molecular, and biochemical features, including shrinkage of cells, blebbing of cells and nuclear membranes, compaction and condensation of chromatin toward the nuclear periphery, and fragmentation of DNA into oligonucleosomes [15] .…”

Section: Introductionmentioning

confidence: 99%

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Nano-cerium-element-doped titanium dioxide induces apoptosis of Bel 7402 human hepatoma cells in the presence of visible light

Wang¹,

Mao²,

Gaohua³

et al. 2007

WJG

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AIM:To investigate the apoptotic effect of photoexcited titanium dioxide (TiO2) nanoparticles in the presence of visible light on human hepatoma cell line (Bel 7402) and to study the underlying mechanism.METHODS: Cerium-element-doped titanium dioxide nanoparticles were prepared by impregnation method. Bel 7402 human hepatoma cells were cultured in RPMI 1640 medium in a humidified incubator with 50 mL/L CO2 at 37℃. A 15 W fluorescent lamp with continuous wavelength light was used as light source in the photocatalytic test. Fluorescence morphology and agarose gel eletrophoresis pattern were performed to analyze apoptotic cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nano-cerium-element-doped titanium dioxide induces apoptosis of Bel 7402 human hepatoma cells in the presence of visible light

Wang¹,

Mao²,

Gaohua³

et al. 2007

WJG

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show abstract

“…Because little is known about the toxicity profile of nanomaterials, no benchmarks or ''safe'' levels have been set for the concern of human health. Presently, more and more researchers have investigated the toxicological effects and potential environmental impacts of nanomaterials, such as single-walled carbon nanotubes (Cui et al, 2005;Warheit et al, 2004), multi-walled carbon nanotubes (Monteiro-Riviere et al, 2005), fullerenes (Chen et al, 1998;Oberdö rster, 2004;Sayes et al, 2005), ultrafine titanium dioxide (Nakagawa et al, 1997;Rahman et al, 2002), quantum dots (Derfus et al, 2004;Green and Howman, 2005), and some transition metals like Cu, Au, Ag, Zn, and their oxides (Goodman et al, 2004;Chen et al, 2006). However, limited available toxicological information on nanomaterials renders people to endure a high risk of using these novel materials, especially in biological and medical applications.…”

Section: Introductionmentioning

confidence: 99%

Effects of waterborne nano-iron on medaka (Oryzias latipes): Antioxidant enzymatic activity, lipid peroxidation and histopathology

Zhou

et al. 2009

Ecotoxicology and Environmental Safety

186

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a b s t r a c tToxicity tests were performed to investigate possible harmful effects on medaka exposed to nano-iron. Dose-dependent decreases of superoxide dismutase (SOD) and increases of malondialdehyde (MDA) were induced in the medaka embryo, suggesting that oxidative damage was induced by nano-iron. For adult medaka, the disturbance of antioxidative balance was observed during the early exposure period based on the monitoring of the hepatic and cerebral SOD and reduced glutathione (GSH). No terminal oxidative damage occurred during the whole exposure period, probably due to the high self-recovering capability of the adult fish. Some histopathological and morphological alterations (cell swelling, hyperplasia, and granulomas, etc.) were observed in gill and intestine tissues, which confirmed that deleterious effects occurred as a result of direct contact with nano-iron. It is suggested that further evaluation should be made concerning the risk assessment of waterborne nano-iron on aquatic life.

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“…Rahman et al (17) also showed the production of reactive oxygen species (ROS) in human and rat alveolar macrophages after exposure to UF-TiO 2 using a luminol-dependent chemiluminescence assay. Nakagawa et al (18) reported the photogenotoxic potential of TiO 2, using different in vitro genotoxicity assays and found that without ultraviolet (UV)/visible light it is weakly mutagenic, but combined with irradiation it is strongly mutagenic. Furthermore Lu et al (19) reported the potential of TiO 2 to induce micronuclei (MN) sister chromatid exchanges in Chinese hamster ovary cells.…”

mentioning

confidence: 99%

Evidence that ultrafine titanium dioxide induces micronuclei and apoptosis in Syrian hamster embryo fibroblasts.

Rahman

Lohani

Dopp

et al. 2002

Environ Health Perspect

355

218

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Inhaled ultrafine titanium dioxide (UF-TiO2) particles cause pronounced pulmonary inflammation, in contrast to fine TiO2. Previous studies provide evidence for the production of reactive oxygen species by alveolar macrophages, after overloading with UF-TiO2 particles and cytotoxicity of UF-TiO2 in rat lung alveolar macrophages. UF-TiO2 also causes pulmonary fibrosis and lung tumors in rats. UF-TiO2 particles are photogenotoxic, but in general, information on the genotoxicity of UF-TiO2 is still limited. We studied the potential of UF-TiO2 (particle size less than or equal to 20 nm) and fine TiO2 (particle size > 200 nm) to induce chromosomal changes, which can be monitored by the formation of micronuclei (MN) in Syrian hamster embryo (SHE) cells. We also analyzed UF-TiO2-treated cells for apoptosis induction. The MN assay revealed a significant increase in MN induction (p less than or equal to 0.05) in SHE cells after treatment with UF-TiO2 (1.0 micro g/cm2) for 12 hr (mean, 24.5 MN/1,000 cells), 24 hr (mean, 31.13 MN/1,000 cells), 48 hr (mean, 30.8 MN/1,000 cells), 66 hr (mean, 31.2 MN/1,000 cells), and 72 hr (mean, 31.3 MN/1,000 cells). Bisbenzimide staining of the fixed cells revealed typical apoptotic structures (apoptotic bodies), and the apoptosis-specific "DNA ladder pattern" resulting from internucleosomal cleavage was identified by gel electrophoresis. Furthermore, transmission electron microscopy of the exposed cells revealed the typical chromatin compaction of apoptosis.

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The photogenotoxicity of titanium dioxide particles

Cited by 183 publications

References 16 publications

Nano-cerium-element-doped titanium dioxide induces apoptosis of Bel 7402 human hepatoma cells in the presence of visible light

Nano-cerium-element-doped titanium dioxide induces apoptosis of Bel 7402 human hepatoma cells in the presence of visible light

Effects of waterborne nano-iron on medaka (Oryzias latipes): Antioxidant enzymatic activity, lipid peroxidation and histopathology

Evidence that ultrafine titanium dioxide induces micronuclei and apoptosis in Syrian hamster embryo fibroblasts.

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