The photolyase gene from the plant pathogen Fusarium oxysporum f. sp. lycopersici is induced by visible light and α-tomatine from tomato plant

Regulation by light in Fusarium

“…The mRNA levels for the phr1 photolyase gene of F. oxysporum, barely detectable in the dark, are rapidly induced by light (Alejandre-Durán et al, 2003). A similar regulation by light was observed for the orthologous phr1 gene in T. harzianum, where the expression is associated to conidiation (Berrocal-Tito et al, 1999).…”

“…As other fungi, Fusarium has a single CPD photolyase (Table 1), and lacks (6-4) photolyase. The CPD photolyase has been investigated in F. oxysporum, where it is encoded by the gene phr1 (Alejandre- Durán et al, 2003). The predicted PHR1 protein is highly similar to their orthologous CPD photolyases from other ascomycetes, such as N. crassa, and its sequence suggests the use of MTHF as a cofactor in addition to FAD.…”

“…The predicted PHR1 protein is highly similar to their orthologous CPD photolyases from other ascomycetes, such as N. crassa, and its sequence suggests the use of MTHF as a cofactor in addition to FAD. The Fusarium PHR1 enzyme shows a highly efficient photoreactivation activity, revealed by the dramatic recovery of the survival of UVtreated cells upon incubation under white light, either for F. fujikuroi (Avalos et al, 1985) or F. oxysporum (Alejandre-Durán et al, 2003).…”

“…Interestingly, photolyase expression is stimulated by oxidative stress in animal cells (Mitani and Shima, 1995). The phr1 promoter contains separate STRE consensus elements that could be responsible of alpha-tomatine induction, and a putative APE element presumably involved in light regulation (Alejandre-Durán et al, 2003). However, a possible connection between the regulation by light of phr1 expression and oxidative stress has not been discarded.…”

Photobiology in the Zygomycota: Multiple photoreceptor genes for complex responses to light

“…For example, fungi growing in dark or shaded areas use light as a signal to promote vegetative reproduction and to direct the growth of reproductive structures towards open air to facilitate spore dispersal (Corrochano and Avalos, 2010;Corrochano and Galland, 2006). In addition, the harmful effects of light, particularly UV light, is reduced after the activation by light of the biosynthesis of screening pigments, like carotenoids, and the activation by light of genes for DNA repair (Alejandre-Durán et al, 2003;Berrocal-Tito et al, 1999;Corrochano and Avalos, 2010).…”