The photometric titration of Fe(III) with EDTA using tiron indicator

Berger, Selman A.; McKay, James

doi:10.1007/bf01218204

Cited by 8 publications

(3 citation statements)

References 10 publications

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“…Incubation of the blot with peptide (2 g/ml) with the anti-DMT-1 antibody before development of the chemiluminescence ablated the band, but other nonspecific peptides had no effect. impermeable specific ferric iron chelator 4,5-dihydroxy-1,3 benzene disulfonic acid (Tiron) to inhibit iron uptake into K562 cells (1,2,5).…”

Section: Resultsmentioning

confidence: 99%

“…The antibody produced no inhibition of iron uptake from solutions containing ferrous iron maintained in the appropriate valence by ascorbate and 2-mercaptoethanol. The lack of inhibition by Tiron, an impermeable ferric iron chelator (1,2,5), indicated that the iron remained in the ferrous valance and that Tiron did not have a nonspecific toxic effect on the cells. This was in marked contrast to the effect of anti-␤ 3 -integrin antibody on uptake of ferric iron (as the citrate chelate).…”

Section: Resultsmentioning

confidence: 99%

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Separate pathways for cellular uptake of ferric and ferrous iron

Conrad

Umbreit

Moore

et al. 2000

American Journal of Physiology-Gastrointestinal and Liver Physi

113

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Separate pathways for transport of nontransferrin ferric and ferrous iron into tissue cultured cells were demonstrated. Neither the ferric nor ferrous pathway was shared with either zinc or copper. Manganese shared the ferrous pathway but had no effect on cellular uptake of ferric iron. We postulate that ferric iron was transported into cells via beta(3)-integrin and mobilferrin (IMP), whereas ferrous iron uptake was facilitated by divalent metal transporter-1 (DMT-1; Nramp-2). These conclusions were documented by competitive inhibition studies, utilization of a beta(3)-integrin antibody that blocked uptake of ferric but not ferrous iron, development of an anti-DMT-1 antibody that blocked ferrous iron and manganese uptake but not ferric iron, transfection of DMT-1 DNA into tissue culture cells that showed enhanced uptake of ferrous iron and manganese but neither ferric iron nor zinc, hepatic metal concentrations in mk mice showing decreased iron and manganese but not zinc or copper, and data showing that the addition of reducing agents to tissue culture media altered iron binding to proteins of the IMP and DMT-1 pathways. Although these experiments show ferric and ferrous iron can enter cells via different pathways, they do not indicate which pathway is dominant in humans.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Separate pathways for cellular uptake of ferric and ferrous iron

Conrad

Umbreit

Moore

et al. 2000

American Journal of Physiology-Gastrointestinal and Liver Physi

113

View full text Add to dashboard Cite

show abstract

“…Tiron yields a higher extinction coefficient in the visible range relative to other iron chelators such as BPTD and EDTA. 24 Following cluster transport across the membrane bilayer, the concentration of iron was readily and independently quantified following release by acidification of the proteoliposome product with concentrated HCl. The product was then neutralized by addition of NaOH and buffered with MES for tiron chelation detection, a complexing agent selective for ferric ions.…”

mentioning

confidence: 99%