The photomorphogenetic reaction of pea plants (Pisum sativum L.) on ultraviolet irradiation effect

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Aim. The effect of chronic ultraviolet B (UV-B) radiation and cytokinin benzylaminopurine (6-BAP) on the growth, content of photosynthetic pigments and hydrogen peroxide (HP) in leaves of pea plants (Pisum sativum L.) was studied. Methods. Pea plants cultivar Gotivsky were irradiated by chronic UV-B at a dose of 3.5 kJ/m2 per day during 5 days. Groups of the irradiated and non-irradiated plants were treated with 6-BAP after the end of the UV-B radiation. Length of shoots and roots of pea plants were measured during all time of experiment. Content of photosynthetic pigments and HP in leaves were determined during the experiment. Results. It was shown that plant growth delayed, content of photosynthetic pigments reduced, content of HP increased in the leaves after the chronic UV-B radiation of pea plants. The content of pigments increased and content of HP stabilized in pea leaves after the treatment of plants with 6-BAP. Conclusions. It was shown that chronic UV-B radiation of pea plants by dose of 3.5 kJ/m2 per day caused inhibition of plants growth, increasing HP content, decreasing photosynthetic pigments content in mature leaves. The BAP treatment of irradiated plants stimulated the restoration of photosynthetic pigments content in leaves.

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The response of pea plants to ultraviolet B radiation and cytokinin

Zhuk,

Mikheev,

Ovsyannikova

2023

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“…УФ-В промені у високих дозах виявили деструктивну дію на молекули ДНК, білків, ліпідів, фотосинтетичні пігменти, мембрани, що здатне затримували ріст та розвиток культурних рослин, зменшувати їх продуктивність у природних умовах вирощування [3]. Проведені нами раніше дослідження дії гострого опромінення УФ-В і УФ-С та хронічного опромінення УФ-В на рослини гороху у широкому діапазоні доз виявили, що ультрафіолетове випромінювання у дозах, які перевищували 4 кДж/м 2 на день, необернено інгібувало ріст рослин гороху у висоту та наростання їх маси, зменшувало кількість фотосинтетичних пігментів у листках, підвищувало вміст ПВ, активність антиоксидантних ферментів, знижувало продуктивність рослин гороху [4][5][6][7]. Гостре опромінення УФ-В на рослини кукурудзи у дозах 1-16 кДж/м 2 викликало зменшення висоти рослин, стимулювало наростання їх маси, накопичення фотосинтетичних пігментів та збільшення вмісту ПВ у листках [8].…”

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Interaction of chronic ultraviolet radiation and cytokinin in adaptive reactions of pea plants

Жук¹,

Mikheev²,

Ovsyannikova³

2020

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Aim. The goal of this study was to study the interaction of chronic ultraviolet (UV-B) radiation and cytokinin 6-benzylaminopurine (BAP) in the adaptation of pea plants (Pisum sativum L.) to the effect of chronic UV-B irradiation. Methods. Pea plants cultivar Aronis were sprayed by BAP solution in concentration 10-5 M before the effect of chronic UV-B radiation at doses of 6 kJ/m2 per day with a power of 1 W/m2 for 12 days. During this time, the growth of plants and their mass were measured, the content of photosynthetic pigments and endogenous hydrogen peroxide (HP) were determined in the leaves. Results. It was established that the effect of chronic UV-B radiation at a dose of 6 kJ/m2 per day on pea plants caused a delay in plant mass accumulation, synthesis of photosynthetic pigments and increasing HP content in leaves. Treatment plants by BAP increased mass, stimulated synthesis of photosynthetic pigments, reduced the content of HP in the leaves of pea plants during the UV-B radiation. Conclusions. It was shown that treatment of pea plants by BAP caused the adaptation of pea plants at the beginning of UV-B radiation. Cytokinins are capable to detoxify reactive oxygen spices, stimulate formation of photosynthetic complex that promotes growth of pea plants under chronic UV-B radiation. Keywords: UV-B, BAP, Pisum sativum L., photosynthetic pigments, adaptation.

“…Проведені нами раніше дослідження показали, що молоді рослини кукурудзи потребують адаптації до дії невисокої дози хронічного УФ-В, що спричиняла тимчасову затримку росту листків, синтез фотосинтетичних пігментів, підвищення вмісту ПВ [5]. Вивчення дії високих доз гострого УФ-В нами проводилося на рослинах гороху, який більш чутливий до ультрафіолету порівняно з кукурудзою [6][7][8]. Встановлено, що опромінення УФ-В рослин гороху у дозах, які перевищували 4 кДж/м 2 , призводило до незворотного інгібування росту рослин, зменшення їх продуктивності, вмісту фотосинтетичних пігментів у листках, збільшення концентрації ПВ.…”

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Adaptive reactions of corn plants to acute ultraviolet radiation

Жук¹,

Mikheyev²,

Ovsyannikova³

2019

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Aim. The goal of this work was to study the adaptive reaction of young corn plants (Zea mays L.) to the action of acute ultraviolet B (UV-B) radiation in the vegetative growth period. Methods. Corn plants hybrid Dostatok 300 MV in the phase of two leaves were irradiated by UV-B at doses of 1, 2, 4, 8, 16 kJ/m2 with a power of 6 W/m2 and were grown in a long day conditions. After irradiation, the growth of shoots in length, the growth of the mass of plants, the content of photosynthetic pigments and endogenous hydrogen peroxide (HP) in the leaves were determined. Results. It is shown that after irradiation of corn plants UV-B at doses of 4, 8, 16 kJ/m2 the growth of shoots in length decreased, after the irradiation of UV-B at doses of 1, 2 kJ/m2 mass of plants increased. UV-B irradiation in all doses, except for a dose of 16 kJ/m2, stimulated the accumulation of photosynthetic pigments in the leaves. Increasing HP content in the leaves was determined in corn leaves after irradiation of UV-B in all doses. Conclusions. It is shown that irradiation of young corn plants by acute UV-B at doses from 1 to 16 kJ/m2 induced adaptive reactions, that included inhibition of shoot growth with simultaneous stimulation of plants mass growth, accumulation of photosynthetic pigments, increased endogenous content of HP. The adaptive period lasted about 10 days, after that processes of growth, formation of a photosynthetic apparatus and content of HP were stabilized. UV-B belongs to the necessary components of sunlight components for corn growth. Keywords: UV-B radiation, Zea mays L., photosynthetic pigments, adaptation.