The photomorphogenic factors UV-B RECEPTOR 1, ELONGATED HYPOCOTYL 5, and HY5 HOMOLOGUE are part of the UV-B signalling pathway in grapevine and mediate flavonol accumulation in response to the environment

Loyola, Rodrigo; Herrera, Daniela P; Más, Abraham; Wong, D.; Höll, Janine; Cavallini, Erika; Amato, Alessandra; Azuma, Akifumi; Ziegler, Tobias; Aquea, Felipe; Castellarin, Simone D.; Bogs, Jochen; Tornielli, Giovanni Battista; Peña‐Neira, Álvaro; Czemmel, Stefan; Alcalde, José Antonio; Matus, José Tomás; Arce‐Johnson, Patricio

doi:10.1093/jxb/erw307

Cited by 106 publications

(130 citation statements)

References 73 publications

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“…Our recent work proposed that VviHYH may fulfil both co-operative and partially redundant roles with VviHY5 during both grapevine photomorphogenic growth, berry development, and under stress 29 , 67 . Supporting this, many genes involved in light- and chloroplast-related functions were densely connected the VviHYH subnetwork ( k = 2) of Module I (Fig.…”

Section: Resultsmentioning

confidence: 62%

“…The strong presence of the G-box CRE may imply a co-operative role of other bZIP TFs in its regulation, such as HY5 51 . This has been shown for VviGT5 , which is up-regulated in a grape HY5 transient expression assay, 29 although VviHYH might also be involved in VviGT5 regulation. Expression of module genes was largely down-regulated during the early stages (12–24 hours post-inoculation, hpi) of leaves infected with P. viticola and E. necator , spider-mites, as well as berries infected with B. cinerea at the late stages of berry development (Fig.…”

Section: Resultsmentioning

confidence: 87%

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Genome-wide analysis of cis-regulatory element structure and discovery of motif-driven gene co-expression networks in grapevine

Wong

Gutierrez

Gambetta

et al. 2017

DNA Res

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Coordinated transcriptional and metabolic reprogramming ensures a plant’s continued growth and survival under adverse environmental conditions. Transcription factors (TFs) act to modulate gene expression through complex cis-regulatory element (CRE) interactions. Genome-wide analysis of known plant CREs was performed for all currently predicted protein-coding gene promoters in grapevine (Vitis vinifera L.). Many CREs such as abscisic acid (ABA)-responsive, drought-responsive, auxin-responsive, and evening elements, exhibit bona fide CRE properties such as strong position bias towards the transcription start site (TSS) and over-representation when compared with random promoters. Genes containing these CREs are enriched in a large repertoire of plant biological pathways. Large-scale transcriptome analyses also show that these CREs are highly implicated in grapevine development and stress response. Numerous CRE-driven modules in condition-specific gene co-expression networks (GCNs) were identified and many of these modules were highly enriched for plant biological functions. Several modules corroborate known roles of CREs in drought response, pathogen defense, cell wall metabolism, and fruit ripening, whereas others reveal novel functions in plants. Comparisons with Arabidopsis suggest a general conservation in promoter architecture, gene expression dynamics, and GCN structure across species. Systems analyses of CREs provide insights into the grapevine cis-regulatory code and establish a foundation for future genomic studies in grapevine.

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Section: Resultsmentioning

confidence: 62%

Section: Resultsmentioning

confidence: 87%

Genome-wide analysis of cis-regulatory element structure and discovery of motif-driven gene co-expression networks in grapevine

Wong

Gutierrez

Gambetta

et al. 2017

DNA Res

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“…Another gene worth mentioning for the highest correlation of expression with VvWRKY26 is UV RESISTANCE LOCUS 8 ( UVR8 ), a photoreceptor that induces changes in gene expression after absorption of UV-B rays mediating the photomorphogenic response (Binkert et al, 2014; Carbonell-Bejerano et al, 2014; Liu et al, 2015; Yin et al, 2015; Loyola et al, 2016). …”

Section: Resultsmentioning

confidence: 99%

A Grapevine TTG2-Like WRKY Transcription Factor Is Involved in Regulating Vacuolar Transport and Flavonoid Biosynthesis

et al. 2017

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A small set of TTG2-like homolog proteins from different species belonging to the WRKY family of transcription factors were shown to share a similar mechanism of action and to control partially conserved biochemical/developmental processes in their native species. In particular, by activating P-ATPases residing on the tonoplast, PH3 from Petunia hybrida promotes vacuolar acidification in petal epidermal cells whereas TTG2 from Arabidopsis thaliana enables the accumulation of proanthocyanidins in the seed coat. In this work we functionally characterized VvWRKY26 identified as the closest grapevine homolog of PhPH3 and AtTTG2. When constitutively expressed in petunia ph3 mutant, VvWRKY26 can fulfill the PH3 function in the regulation of vacuolar pH and restores the wild type pigmentation phenotype. By a global correlation analysis of gene expression and by transient over-expression in Vitis vinifera, we showed transcriptomic relationships of VvWRKY26 with many genes related to vacuolar acidification and transport in grapevine. Moreover, our results indicate an involvement in flavonoid pathway possibly restricted to the control of proanthocyanidin biosynthesis that is consistent with its expression pattern in grape berry tissues. Overall, the results show that, in addition to regulative mechanisms and biological roles shared with TTG2-like orthologs, VvWRKY26 can play roles in fleshy fruit development that have not been previously reported in studies from dry fruit species. This study paves the way toward the comprehension of the regulatory network controlling vacuolar acidification and flavonoid accumulation mechanisms that contribute to the final berry quality traits in grapevine.

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“…6. Genes that were identified as low-temperature-inducible, light-inducible, and low-temperature plus light-inducible in 'Pione' (Azuma et al, 2015b (Loyola et al, 2016). In addition, the grape berry UV-B response machinery favors flavonoid accumulation by activating HY5 in response to UV-B exposure.…”

Section: Aba Signalingmentioning

confidence: 99%

Genetic and Environmental Impacts on the Biosynthesis of Anthocyanins in Grapes

Azuma

2018

The Hortic J

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Because of the commercial importance of grapes (Vitis spp.), it is important to understand how grape coloration is affected by genetic and environmental factors, as this knowledge may contribute to more stable production of high-quality grapes. The color of berry skins is determined mainly by the quantity and composition of anthocyanins. This review summarizes the results of recent studies of the genetic and environmental regulation of anthocyanin biosynthesis in grape berry skin: (i) The myeloblastosis (MYB) haplotype composition at the color locus is the major genetic factor that determines the anthocyanin content.(ii) The MYB haplotype composition at the color locus and the anthocyanin O-methyltransferase locus are major genetic determinants of the ratios of tri-to di-hydroxylated anthocyanins and of methylated to nonmethylated anthocyanins. (iii) The accumulation of anthocyanins depends on both low temperature and light, and the two factors have a synergistic effect on the expression of genes within the anthocyanin biosynthesis pathway. (iv) Comprehensive transcriptome analysis using a grape oligo-DNA microarray let my research group identify many candidate genes involved in low-temperature-induced abscisic acid signaling and light signaling networks related to anthocyanin accumulation in grape berry skin. These findings will allow prediction of the skin color of grapes from seedlings at a very young stage by examining the MYB haplotype composition. Furthermore, these results will contribute to a fuller understanding of how grape coloration is affected by environmental factors, thereby helping grape growers to develop cultivation techniques that contribute to the production of highly pigmented grapes.

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The photomorphogenic factors UV-B RECEPTOR 1, ELONGATED HYPOCOTYL 5, and HY5 HOMOLOGUE are part of the UV-B signalling pathway in grapevine and mediate flavonol accumulation in response to the environment

Abstract: By performing molecular studies coupled to radiation experiments and in silico systems analyses, we have ascertained the role of the grapevine UV-B receptor and two HY5 homologues in regulating flavonol synthesis.

Cited by 106 publications

References 73 publications

Genome-wide analysis of cis-regulatory element structure and discovery of motif-driven gene co-expression networks in grapevine

Genome-wide analysis of cis-regulatory element structure and discovery of motif-driven gene co-expression networks in grapevine

A Grapevine TTG2-Like WRKY Transcription Factor Is Involved in Regulating Vacuolar Transport and Flavonoid Biosynthesis

Genetic and Environmental Impacts on the Biosynthesis of Anthocyanins in Grapes

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