The photoreceptor UVR8 mediates the perception of both UV-B and UV-A wavelengths up to 350 nm of sunlight with responsivity moderated by cryptochromes

Rai, Neha; O’Hara, Andrew; Farkas, Daniel L.; Safronov, Omid; Ratanasopa, Khuanpiroon; Wang, Fang; Lindfors, Anders V.; Jenkins, Gareth I.; Lehto, Tarja; Salojärvi, Jarkko; Brosché, Mikael; Strid, Åke; Aphalo, Pedro J.; Morales, Luis Orlando

doi:10.1101/2020.01.21.913814

Cited by 8 publications

(18 citation statements)

References 65 publications

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“…Furthermore, many research groups have reported that flavonoids have a function in defense against drought, when acting as free radical scavengers (Ramakrishna & Ravishankar, 2011; Nakabayashi et al, 2014; Kumar et al, 2020). Our data is in agreement with previous research which showed that UV-B enhances accumulation of CHS transcripts, representing first committed enzyme of the flavonoid biosynthesis pathway (Brown & Jenkins, 2008; Rodríguez-Calzada et al, 2019; Rai et al,. 2020; Santin et al, 2020).…”

Section: Discussionsupporting

confidence: 94%

“…To further study the influence of UV-B under the different treatments, we measured changes in transcript accumulation of a set of UV-B marker genes. The UV-B photoreceptor UVR8 (Jenkins, 2014) mediates responses to UV-B through interactions with specific signaling components and transcription factors which ultimately lead to the biosynthesis of phenolic compounds, such as flavonoids (Hideg, Jansen & Strid, 2013; Hideg & Strid, 2017; Jenkins, 2014; Liang, Yang & Liu, 2019; Rai et al, 2020). Transcript accumulation of UVR8 was strongly increased in UV-B-treated tobacco plants on day 14.…”

Section: Discussionmentioning

confidence: 99%

“…In response to UV-B, the expression of BES1 in Arabidopsis appears to be dependent on the nature of the UV-B treatment. While UV-B levels in natural sunlight (1.5 μmol m -2 s -1 for 6 hours) did not affect BES1 transcript accumulation in wild type plants (Rai et al, 2020), exposure to UV-B stress provided by broad-band UV (5 μmol m -2 s -1 for 8 hours for 10 days) lowers BES1 transcript levels (Liang et al, 2020). Here we show that low level UV-B-pre-treatment led to increased BES1 transcript levels in tobacco plants (Fig.…”

Section: Discussionmentioning

confidence: 99%

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UV-B exposure and exogenous hydrogen peroxide application leads to cross-tolerance toward drought in Nicotiana tabacum L

Morales

Strid

et al. 2021

Preprint

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Acclimation of plants to water deficit involves biochemical and physiological adjustments. Here, we studied how UV-B exposure and exogenously applied hydrogen peroxide (H2O2) potentiates drought tolerance in tobacco (Nicotiana tabacum L.). Separate and combined applications for 14 days of 1.75 kJ m−2 day−1 UV-B radiation and 0.2 mM H2O2 were assessed. Both factors, individually and combined, resulted in inhibition of growth. Furthermore, the combined treatment led to the most compacted plants. UV-B- and UV-B+H2O2-treated plants increased total antioxidant capacity and foliar epidermal flavonol content. H2O22- and UV-B+H2O2-pre-treated plants showed cross-tolerance to a subsequent 7-day drought treatment. Plant responses to the pre-treatment were notably different: i) H2O2 increased the activity of catalase, phenylalanine ammonia lyase and peroxidase activities, and ii) the combined treatment induced epidermal flavonols which were key to drought tolerance. We report synergistic effects of UV-B and H2O2 on transcription accumulation of UV RESISTANCE LOCUS 8, NAC DOMAIN PROTEIN 13 (NAC13), and BRI1-EMS-SUPPRESSOR 1 (BES1). Our data demonstrate a pre-treatment-dependent response to drought for NAC13, BES1 and CHALCONE SYNTHASE transcript accumulation. This study highlights the potential of combining UV-B and H2O2 to improve drought tolerance which could become a useful tool to reduce water use.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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UV-B exposure and exogenous hydrogen peroxide application leads to cross-tolerance toward drought in Nicotiana tabacum L

Morales

Strid

et al. 2021

Preprint

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“…This research confirms that different photoreceptors trigger responses governed by UV radiation shorter than 350 nm and responses mediated by UV radiation longer than 350 nm and blue light. However, these two sets of responses interact with each other to coordinate acclimation to high light vs shade conditions [ 170 – 173 ]. These findings imply that perception and response to UV-B radiation not only protects against the deleterious effects of very high solar radiation but that many of these photoprotective responses can also be induced by UV-A radiation and blue light.…”

Section: Terrestrial Ecosystems and Biodiversitymentioning

confidence: 99%

Environmental effects of stratospheric ozone depletion, UV radiation, and interactions with climate change: UNEP Environmental Effects Assessment Panel, Update 2020

Neale

Barnes

Robson

et al. 2021

Photochem Photobiol Sci

130

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This assessment by the Environmental Effects Assessment Panel (EEAP) of the United Nations Environment Programme (UNEP) provides the latest scientific update since our most recent comprehensive assessment (Photochemical and Photobiological Sciences, 2019, 18, 595–828). The interactive effects between the stratospheric ozone layer, solar ultraviolet (UV) radiation, and climate change are presented within the framework of the Montreal Protocol and the United Nations Sustainable Development Goals. We address how these global environmental changes affect the atmosphere and air quality; human health; terrestrial and aquatic ecosystems; biogeochemical cycles; and materials used in outdoor construction, solar energy technologies, and fabrics. In many cases, there is a growing influence from changes in seasonality and extreme events due to climate change. Additionally, we assess the transmission and environmental effects of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is responsible for the COVID-19 pandemic, in the context of linkages with solar UV radiation and the Montreal Protocol.

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“…UV-B signaling requires monomerization of UVR8, which forms a homodimer in the ground state (Rizzine et al, 2011). The absorption spectrum of UVR8 has a peak at 280 nm and extends from at least 250 nm to around 500 nm (Rai et al, 2020). Monomerization of UVR8 occurs under UV in the range from 260 to 335 nm (Díaz-Ramos et al, 2018;Rai et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and metabolome analyses revealed that narrowband 280 and 310 nm UV-B induce distinctive responses in Arabidopsis

Tsurumoto

Fujikawa²,

Ohta

et al. 2021

Preprint

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In plants, the UV-B photoreceptor UV RESISTANCE LOCUS8 (UVR8) perceives UV-B and induces UV-B responses including synthesis of UV-B absorbing phenolic compounds such as anthocyanins. UVR8 absorbs a range of UV-B (260–335 nm). However, the responsiveness of plants to each UV-B wavelength has not been intensively studied so far. Here, we performed transcriptome and metabolome analyses of Arabidopsis using UV light emitting diodes (LEDs) with peak wavelengths of 280 and 310 nm to investigate the differences in the wavelength-specific UV-B responses. Irradiation with both UV-LEDs induced gene expression of the transcription factor ELONGATED HYPOCOTYL 5 (HY5), which has a central role in the UVR8 signaling pathway. However, the overall transcriptomic and metabolic responses to 280 and 310 nm UV-LED irradiation were different. Most of the known UV-B-responsive genes, such as salicylic acid, jasmonic acid, and defense-related genes, responded only to 280 nm UV-LED irradiation. Lipids, polyamines and organic acids were the metabolites most affected by 280 nm UV-LED irradiation, whereas the effect of 310 nm UV-LED irradiation on the metabolome was considerably less. Enzymatic genes involved in the phenylpropanoid pathway upstream in anthocyanin biosynthesis were up-regulated only by 280 nm UV-LED irradiation. On the other hand, no enzymatic genes downstream in anthocyanin biosynthesis were induced by the UV-LEDs, but rather, they were down-regulated by 310 nm UV-LED irradiation. These results revealed that the responsivenesses of Arabidopsis to 280 and 310 nm UV-B were significantly different, suggesting that UV-B signaling is mediated by more complex pathways than the current model.

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The photoreceptor UVR8 mediates the perception of both UV-B and UV-A wavelengths up to 350 nm of sunlight with responsivity moderated by cryptochromes

Cited by 8 publications

References 65 publications

UV-B exposure and exogenous hydrogen peroxide application leads to cross-tolerance toward drought in Nicotiana tabacum L

UV-B exposure and exogenous hydrogen peroxide application leads to cross-tolerance toward drought in Nicotiana tabacum L

Environmental effects of stratospheric ozone depletion, UV radiation, and interactions with climate change: UNEP Environmental Effects Assessment Panel, Update 2020

Transcriptome and metabolome analyses revealed that narrowband 280 and 310 nm UV-B induce distinctive responses in Arabidopsis

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