Introduction Establishing new apple orchards using well-feathered apple trees has recently become common practice. In favourable growing conditions, these trees can produce high yield in the second leaf, achieving full productivity in the fourth leaf (Radivojevic et al., 2014). High-quality trees of the cultivars 'Gala' and 'Golden Delicious' during the establishing period frequently produce an excessive number of fruitlets but this can be insufficient to guarantee fruit of good marketable size at harvest (Bregoli et al., 2007). Furthermore, an unwanted excessive number of fruit per tree during that period can reduce the growth of a young tree and delay the achievement of maximum tree size. Because of this, trees require adequate thinning to reach marketable fruit sizes and regular yields by preventing alternate bearing (Maas, 2006). In mature orchards chemical fruit thinning is a major cultural practice (Bound, 2006; Dorigoni and Lezzer, 2007), but in young apple orchards hand fruit thinning is usually done. However, due to the increasing lack of seasonal workers and high labour costs, this job is becoming unrealisable, especially in large areas (Radivojević et al., 2011). Moreover, hand thinning is usually carried out after June-drop, by which time only increases in fruit size are achieved and there is no reduction in alternate bearing (Maas, 2006). Chemical thinning, as compared to hand thinning, is a quick operation and allows thinning fruit at the right moment, guaranteeing better fruit quality and significantly reducing labour costs (Costa et al., 2006). In young apple orchards chemical thinning has to be regularly performed and start in the second leaf, whereby different chemicals can be applied either alone or in combination in order to improve reliability and thinning efficacy (Verjans et al., 2018). Plant growth regulators such as auxins and cytokinins have become essential in commercial apple production (Brunner, 2014; Stern, 2015). However,