The physicochemical aspects of DNA sensing using electrochemical methods

Batchelor‐McAuley, Christopher; Wildgoose, Gregory G.; Compton, Richard G.

doi:10.1016/j.bios.2009.01.045

Cited by 83 publications

(45 citation statements)

References 91 publications

(83 reference statements)

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“…First covalently bound electroactive labels were introduced into DNA in the beginning of the 1980s [12,13] and are currently used to end-label nucleic acids in the DNA hybridization sensors [14][15][16][17]. Labeling of nucleic acids is very useful in the electrochemical analysis of nucleic acids [11,[18][19][20][21][22] but label-free DNA sensors are preferred whenever possible and convenient [11,23,24].…”

Section: Introductionmentioning

confidence: 99%

Square Wave Stripping Voltammetry of Unlabeled Single‐ and Double‐Stranded DNAs

Bartošík

Paleček

2011

Electroanalysis

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We show that, in difference to previously applied electrochemical methods working with stationary electrodes, square wave voltammetry produces well-developed peaks II SW (specific for dsDNA) and III SW yielded by ssDNA at hanging mercury drop electrode (HMDE) and solid amalgam electrodes (SAEs). Using these peaks various kinds of DNA structural transitions can be studied, including unwinding of dsDNA at negatively charged electrode surfaces. The sensitivity of the DNA analysis is much better than that obtained with guanine oxidation signals at carbon electrodes. Both carbon electrodes and SAEs appear attractive as transducers in label-free RNA and DNA sensors.

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Section: Introductionmentioning

confidence: 99%

Square Wave Stripping Voltammetry of Unlabeled Single‐ and Double‐Stranded DNAs

Bartošík

Paleček

2011

Electroanalysis

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“…Labeling step incorporated into nucleic acid assay has shortcomings of limited labeling efficiency, complex multistep analysis, and contamination to samples [32]. Eliminating the tedious labeling process, a GO-organic dye charge transfer complex was fabricated by Loh and coworkers [33].…”

Section: Fluorescent Assaymentioning

confidence: 99%

Graphene for DNA Biosensing

Han

et al. 2014

SpringerBriefs in Molecular Science

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Graphene (or GO) is an excellent candidate for biomolecules anchoring and detection due to its large surface area (up to 2,630 m 2 /g) and unique sp 2 (sp 2 /sp 3 )-bonded network. According to the binding affinity difference between single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) to graphene sheet, GO has been successfully adopted as a platform to discriminate DNA sequences. Fluorescent, electrochemical, electrical, surface-enhanced Raman scattering (SERS) and other methods have been utilized to achieve the sensitive, selective, and accurate DNA recognition. Both theoretical and experimental results illustrate that ssDNA sequences are adsorbed on the surface of graphene sheet with all nucleobases lying nearly flat. DNA or RNA sequencing through graphene nanopore, nanogap, and nanoribbon has also attracted much interest because it is a label-free, amplification-free, and single-molecule approach that can be scaled up for high-throughput DNA or RNA analysis. Meanwhile, miRNA detection is achieved by forming DNA-miRNA duplex helixes, and strong emission is observed due to the poor interaction between the helix and GO.Keywords DNA sequencing Á miRNA detection Á Fluorescent spectroscopy Á Electrochemical method Á Electrical method Á Graphene nanopore Á Graphene nanogap Á Graphene nanoribbon Investigation of DNA and Graphene Binding InteractionThe large surface area (up to 2,630 m 2 /g) and unique sp 2 (sp 2 /sp 3 )-bonded network make graphene (GO) an excellent candidate for biomolecules anchoring and detection. Taking DNA for the first example, various strategies have been adopted to nucleic acid sequence recognition [1][2][3][4][5][6][7][8][9][10]. To better illustrate the interaction, how nucleobases interact with graphene should be addressed, which may be inspired by the interaction of nucleobases with CNTs [11-13] and highly oriented pyrolytic graphite (HOPG) [14].

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“…These have the aim to investigate drug-DNA interaction especially for "genotoxicity" or to determine drug compounds by selective interaction with synthesized oligonucleotides namely "aptasensor" [148][149][150]. Amperometric sensing at an oligonucleotide-modified electrode can be achieved by direct oxidation of guanine [18,151,152] or indirectly using a redox-active label which intercalates preferably at the double stranded configuration [153,154].…”

Section: Oligonucleotide-based Biosensorsmentioning

confidence: 99%