The Physiological Basis for a Method of Assaying Aldosterone in Extracts of Human Urine

Liddle, Grant W.; Cornfield, Jerome; Casper, Alfred; Bartter, Frederic C.

doi:10.1172/jci103190

Cited by 75 publications

(23 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increased potassium excretion often began prior to salt retention, was relatively small in degree, and at times had ceased before the sodium retention had reached its peak. Similar effects of aldosterone have been described both during acute administration (2,7,25,26,(31)(32)(33) and in more prolonged balance studies (1,(3)(4)(5)(6)37).…”

Section: Resultssupporting

confidence: 69%

“…The peak effect occurred in 2 to 4 hours, and the action lasted for as long as 6 to 8 hours after administration. In previous studies of man, other investigators have also noted a similar latent period and a similar duration of activity (3,7,25). Moreover, Barger, Berlin and Tulenko (26) and Ganong, Mulrow and Hollinger (31,32), utilizing renal artery infusions, and Crabbe (27), utilizing an isolated toad bladder, have also noted the delayed onset of action of the hormone.…”

Section: Resultsmentioning

confidence: 65%

See 1 more Smart Citation

The Nature of the Action of Intravenous Aldosterone: Evidence for a Role of the Hormone in Urinary Dilution*

Sonnenblick

Cannon²,

Laragh³

1961

J. Clin. Invest.

View full text Add to dashboard Cite

Aldosterone increases the renal retention of sodium chloride and promotes the excretion of potassium ions ( 1-11 ); but where and how the hormone acts in the renal tubule is poorly understood. The present study was designed to characterize further the mode and site of action of aldosterone in the nephron.Information can be obtained about the site of action of a compound by observing its effect on free water formation (12). According to a current concept of renal physiology (13), reabsorption of solute (sodium chloride) is isosmotic in the proximal tubule (14), whereas, in the more distal segments, reabsorption may or may not be isosmotic (15,16). During water diuresis (absence of antidiuretic hormone) the distal reabsorption of solute is selective, i.e., occurs without isosmotic amounts of water, leaving "solute-free" water behind for excretion (17). Thus, by determining the effect of a compound on solute excretion and on free water formation during maintained water diuresis, information may be obtained about its site of action (12). If aldosterone were to act to promote isosmotic reabsorption of sodium, the consequent reduction in solute excretion would be accompanied by a fall in urine flow and no change or fall in free water excretion. However, if aldosterone acts only at a more distal site, where reabsorption is selective, the reduced sodium excretion would be accompanied by no change in urine flow and hence, by a rise in free water excretion.In the present study it has been shown that aldosterone can promote abstraction of sodium chloride exclusive of water from the tubular urine. This action takes place in the tubule at a locus distal to that of isosmotic reabsorption. Also, this effect of the hormone on sodium chloride reabsorption appears to be dissociated from the effect on potassium excretion. EXPERIMENTALSeven studies were performed on 3 subj ects without evidence of renal or cardiovascular disease. Four of the experiments were done with subj ects maintained on a normal NaCl intake (4 to 6 g NaCl per day for 7 days), two with the subj ects on a higher intake of salt for 7 days (8 to 20 g NaCl per day), and one after drastic salt deprivation (less than 250 mg per day for 6 days). In two subjects on normal salt intake, control studies of similar protocol but without administration of aldosterone were performed. All experiments were conducted in the fasting state at the same early morning hour with the subjects in a recumbent position.Water diuresis was induced by having the patient ingest about 1,500 ml of water by mouth during the hour before the experiment and was subsequently maintained by intravenous infusion of 5 per cent dextrose and water at a constant rate, at least 2 ml per minute greater than urinary output. Adequate control periods were obtained until a steady state of maximum urine flow was demonstrated, so that any subsequent change in free water clearance could not be attributed to an increasing water diuresis.When a steady state of maximum water diuresis had been achieved, 1 mg of d,l-ald...

show abstract

Section: Resultssupporting

confidence: 69%

Section: Resultsmentioning

confidence: 65%

The Nature of the Action of Intravenous Aldosterone: Evidence for a Role of the Hormone in Urinary Dilution*

Sonnenblick

Cannon²,

Laragh³

1961

J. Clin. Invest.

View full text Add to dashboard Cite

show abstract

“…The active, crude extracts of urine consistently have been found to possess activity indistinguishable from pure aldosterone. Other groups have reported similar results (13). Figure 1 illustrates the effect of orally administered KC1 on the concentration of serum potassium in normal and sodium-depleted dogs.…”

Section: Methodssupporting

confidence: 68%

A Study of the Mechanism of Secretion of the Sodium-Retaining Hormone (Aldosterone)12

Laragh¹,

Stoerk²

1957

J. Clin. Invest.

130

View full text Add to dashboard Cite

“…This was to be expected, since such a relationship forms the basis for measurement of aldosterone by bioassay (19)(20)(21)(22). It is noteworthy that, as was the case with the toad bladder, the bioassay data failed to demonstrate a shortening of the latent period between administration of the steroid and the onset of sodium retention when larger amounts of aldosterone were used.…”

mentioning

confidence: 88%

Stimulation of Active Sodium Transport by the Isolated Toad Bladder With Aldosterone in Vitro*

Crabbé¹

1961

J. Clin. Invest.

238

View full text Add to dashboard Cite

The Physiological Basis for a Method of Assaying Aldosterone in Extracts of Human Urine

Cited by 75 publications

References 13 publications

The Nature of the Action of Intravenous Aldosterone: Evidence for a Role of the Hormone in Urinary Dilution*

The Nature of the Action of Intravenous Aldosterone: Evidence for a Role of the Hormone in Urinary Dilution*

A Study of the Mechanism of Secretion of the Sodium-Retaining Hormone (Aldosterone)12

Stimulation of Active Sodium Transport by the Isolated Toad Bladder With Aldosterone in Vitro*

Contact Info

Product

Resources

About