The physiological role of abscisic acid in the rooting of poplar and aspen stump sprouts

Blake, Terence J.; Atkinson, Susan M.

doi:10.1111/j.1399-3054.1986.tb05070.x

Cited by 17 publications

(9 citation statements)

References 13 publications

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“…These data suggest that ABA is inhibitory to root initiation in Cotinus cuttings if the levels of IAA are low . High levels of ABA alone apparently do not inhibit rooting of Cotinus cuttings, a result also reported with Rhododendron [27] and Populus species [4] .…”

Section: Discussionsupporting

confidence: 56%

Endogeneous levels of indole-3-acetic acid and abscisic acid during the rooting of Cotinus coggygria cuttings taken at different times of the year

1991

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Cuttings of Cotinus coggyria cv Royal Purple rooted well in the spring but not at all later in the season . Levels of free and conjugated IAA and ABA were measured in cuttings taken at different times of the year. Hormones were measured in the leaf, the upper stem and the lower stem (rooting zone) . In cuttings taken in early June the level of IAA was much higher than that of conjugated IAA . In late July the opposite was found . No significant differences in ABA levels were found although the ABA/IAA ratio changed dramatically.

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Section: Discussionsupporting

confidence: 56%

Endogeneous levels of indole-3-acetic acid and abscisic acid during the rooting of Cotinus coggygria cuttings taken at different times of the year

1991

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“…Cuttings from easy-to-root poplar hybrids [4], however, contain more ABA than those from difficult-to-root hybrids . The role of ABA in the rooting process has been neglected in most previous research .…”

Section: Discussionmentioning

confidence: 99%

Effects of serial subculture in vitro on the endogenous levels of indole-3-acetic acid and abscisic acid and rootability in microcuttings of ?Jonathan? apple

1992

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Proliferating axillary shoots of the difficult-to-root apple cultivar `Jonathan' acquired an enhanced ability to form adventitious roots with increasing number of subcultures in vitro . The transition between the difficult-to-root and the easy-to-root condition occurred at the fourth subculture .Endogenous levels of free IAA and ABA in shoot tissues were analysed by gas chromatography/mass spectrometry/single ion monitoring (GC/MS/SIM) using negative ion chemical ionisation . Tissues from the mother plants grown in the glasshouse contained more IAA and ABA than those from tissue-culture material . After establishment in vitro there was no variation in the IAA content throughout the subcultures but a decrease in ABA content was observed after the fourth transfer . The IAA/ABA ratio increased from 0 .2 in difficult-to-root shoots from the initial culture up to 0 .7 in easy-to-root shoots from the long-term subculture. 1 . IntroductionThe propagation of apples from cuttings is difficult because most apple cultivars and rootstocks do not readily form roots under conventional nursery conditions [23] . However, the rooting ability of apple cuttings can be improved by `conditioning' the mother plants using treatments such as heavy pruning and blanching [11], application of growth retardants [6,9] or exposure to low light intensity [7] . Success in the striking of cuttings is determined as much by the management of the stock plants prior to severance as by the conditions under which root formation occurred [18] . Most of the research done on root formation is concerned with cuttings after excision from the stock plants .Recent reviews on adventitious root formation [10,13] have drawn attention to the importance of the physiological state of the mother plants at time of collection of the cuttings . Investigations on the hormonal balance in stock and the results are conflicting . In Dahlia [3] and in Rhododendron [24] the capacity of cuttings to form adventitious roots is not correlated with the endogenous level of IAA in the mother plants . In stock plant of Bougainvillea, Hibiscus [5] and Vitis [2], however, enhanced root formation of cuttings is associated with high IAA content . Similarly very little is known about the occurrence of ABA in mother plants . In Rhododendron the rooting ability of cuttings is positively correlated with a high ABA content in stock plants [24] but high ABA levels are found to be inhibitory in Sequoia sempervirens [8] .Difficult-and easy-to-root microcuttings of the same apple cultivar can be produced in vitro by repeated subculture under specific conditions [19] .

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“…ABA was identified by (1) its retention time on the column, (2) co-chromatography with authentic standards (Sigma), and (3) UV isomerization. The identity of ABA in the extract was confirmed by GC-MS, as described previously (Blake and Atkinson 1986).…”

Section: Aba Determinationmentioning

confidence: 99%

Drought tolerance, abscisic acid and electrolyte leakage in fast- and slow-growing black spruce (Picea mariana) progenies

Tan¹,

Blake²

1993

Physiol Plant

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Tan, W. and Blake, T. J. 1993. Drought tolerance, abscisic acid and electrolyte leakage in fast-and slow-growing black spruce (Picea mariana) progenies. -Physiol. Plant. 89: 817-823.To investigate the role that drought tolerance plays in growth, abscisic acid (ABA) accumulation and electrolyte leakage during water stress were compared in fast-and slow-growing black spruce (Picea mariana [Mill.] B. S. P.) progenies. Changes in the ABA content of the needles were quantified using an indirect enzyme-linked immunosorbent assay validated by gas chromatography electron capture detection. Needle electrolyte leakage was estimated using a conductivity bridge. Seedlings were stressed using (I) osmotic stress, induced by a stepwise increase in concentrations of polyethylene glycol 3 350 (PEG) for ABA study and (2) air drying for electrolyte leakage study. Progenies did not differ in ABA levels under unstressed conditions, but progeny differences were observed under osmotic stress. Needle ABA content increased up to 500% under osmotic stress. Slow-growing black spruce progenies (25 and 46) accumulated more ABA under moderate (18% PEG), but not severe (25% PEG), osmotic stress. The slow-growing progenies also leaked more electrolytes under moderate to severe water stress and lost 50% electrolytes at a higher xylem tension, suggesting they suffered more injury and were less dehydration tolerant. Our previously-published results showed that slow-growing progenies lost their photosynthesis and stomatal conductance more quickly during osmotic stress and recovered more slowly after rehydration. Therefore, tolerance of dehydration leading to a maintenance of physiological integrity during drought stress could explain the fast growth rates of more vigorous black spruce progenies.

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The physiological role of abscisic acid in the rooting of poplar and aspen stump sprouts

Cited by 17 publications

References 13 publications

Endogeneous levels of indole-3-acetic acid and abscisic acid during the rooting of Cotinus coggygria cuttings taken at different times of the year

Endogeneous levels of indole-3-acetic acid and abscisic acid during the rooting of Cotinus coggygria cuttings taken at different times of the year

Effects of serial subculture in vitro on the endogenous levels of indole-3-acetic acid and abscisic acid and rootability in microcuttings of ?Jonathan? apple

Drought tolerance, abscisic acid and electrolyte leakage in fast- and slow-growing black spruce (Picea mariana) progenies

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