The phytochemical hyperforin triggers thermogenesis in adipose tissue via a Dlat-AMPK signaling axis to curb obesity

Abstract

Highlights d Use of CMap identified the phytochemical hyperforin as an anti-obesity agent in mice d Hyperforin promotes adipose tissue thermogenesis to promote weight loss d Dlat was identified and validated as a direct molecular target of hyperforin d Hyperforin-induced thermogenesis is modulated by a Dlat-AMPK-PGC1a axis

Camptothecin effectively treats obesity in mice through GDF15 induction

“…With advances in bioinformatics and network pharmacology, the Connectivity Map (CMAP), a database collecting gene expression profiles of drug-treated human cell lines [ 19 ], has been widely used for anti-obesity purpose to screen leptin sensitizer [ 20 , 21 ] and thermogenic activators [ 22 ]. In the current work, we screened the CMAP database for small molecules with gene expression signatures of GDF15 and identified Camptothecin (CPT) as a potential GDF15 inducer.…”

Imatinib and methazolamide ameliorate COVID-19-induced metabolic complications via elevating ACE2 enzymatic activity and inhibiting viral entry

“…The CMAP (https://clue.io/), an online platform which conducts pattern-matching algorithms to compare input gene signatures with gene signatures in diverse contexts of over 450,000 chemical perturbagens (Chen et al, 2021;Subramanian et al, 2017), was utilized to identify compounds that create gene expression patterns opposite to SARS-CoV-2 infection. Six articles reporting transcriptomics of SARS-CoV-2-infected organs or cells (Appelberg et al, 2020;Liao et al, 2020;Liu et al, 2020;Shen et al, 2020;Wang and Xu, 2020;Xiong et al, 2020) were searched out in May 18, 2020, top and bottom 50-150 featured genes were selected from each article and submitted online for CMAP query, and then one ranked list of compounds was generated for each article.…”

Obesity modulates cell-cell interactions during ovarian folliculogenesis

“…We first used two widely corroborated genetic models, leptin knockout (Lep KO) and leptinreceptor knockout (Lepr KO) mice (Figure S1A). Due to leptin deficiency in Lep KO mice or leptin receptor loss-of-function in Lepr KO mice, these two mouse lines developed severe hyperphagia and spontaneous obesity, accompanying the resulting metabolic disorders including insulin-resistance and impaired glucose homeostasis (Chen et al, 2021;Jia et al, 2020;Pan et al, 2021;Tao et al, 2020;Zheng et al, 2021aZheng et al, , 2021b. We also used high-fat diet feeding to obtain HFD mice (Figure S1B).…”