The Phytochemical Profile and Biological Activity of<i>Liquidambar orientalis</i>Mill. var.<i>orientalis</i>via NF-κB and Apoptotic Pathways in Human Colorectal Cancer

Çetinkaya, Sümeyra; Ayan, İlknur Çınar; Süntar, İpek; Dursun, Hatice Gül

doi:10.1080/01635581.2021.1952455

Cited by 12 publications

(5 citation statements)

References 42 publications

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“…20 min later, the chamber was taken out and washed with PBS. Finally, the chamber was taken out and the number of transmembrane cells was then observed and randomly counted in three fields of view per well with three replicate wells set for each group [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

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Effect of Kaempferol on the Biological Behavior of Human Colon Cancer via Regulating MMP1, MMP2, and MMP9

Zhang

Fan

Guo

et al. 2022

Journal of Oncology

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Kaempferol is a kind of flavonoid, which has a significant anticancer effect. MMPs were discovered with the function of cleaving the extracellular matrix. We utilized bioinformatics to analyze the association and bonding mode between the traditional Chinese medicine (TCM) monomer composition (i.e., kaempferol) and the target proteins. The purpose of our research was to verify the effect of kaempferol on the biological behavior of human colon cancer cells HCT116 and HT29 and the expression of matrix metalloproteinase (MMP) 1, 2, and 9 genes. We detected the changes in the biological behavior of colon cancer cells treated with kaempferol by CCK-8, wound healing assay, transwell migration/invasion assay, and flow cytometry. Meanwhile， we detected the expression difference of the target gene by qRT-PCR and western blot. Compared with the two control groups, the cell viability of the kaempferol group decreased, the rate of cell migration and the number of transmembrane cells in the kaempferol group decreased significantly, and the early apoptosis rate increased, the number of cells in the G1 phase increased and in the S phase decreased. The results of qRT-PCR and western blot showed that the expression of target genes MMP1, 2, and 9 in the kaempferol group was lower than that in the two control groups. Kaempferol can significantly inhibit the proliferation, invasion, and migration ability of colon cancer cells; induce their apoptosis; and block the cell cycle. Meanwhile, the expression of MMP1, 2, and 9 genes was downregulated, which verified the results of bioinformatic analysis.

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Section: Methodsmentioning

confidence: 99%

“…Following proper mixing, the tube was incubated away from light at room temperature for 15 min. Finally, flow cytometry was used for analysis [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

Effect of Kaempferol on the Biological Behavior of Human Colon Cancer via Regulating MMP1, MMP2, and MMP9

Zhang

Fan

Guo

et al. 2022

Journal of Oncology

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“…The methanol leaf extract exhibited the highest cytotoxicity in the HCT-116 and HT-29 cell lines, with IC 50 values of 27.80 and 43.13 µg/mL, respectively, compared to all other extracts. The major phytochemicals identified were quercetin 3-glucoside, pyrogallol, chlorogenic acid, apigenin 7-O-glucoside, epigallocatechin gallate, genistein, gallic acid, kaempferol, and luteolin [73].…”

Section: Colorectal Cancer Polyphenols and Identification Techniquesmentioning

confidence: 99%

Dietary Polyphenols: Extraction, Identification, Bioavailability, and Role for Prevention and Treatment of Colorectal and Prostate Cancers

et al. 2022

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Fruits, vegetables, and other edible plants in our diet have numerous health benefits, due to the bioactive compounds in these food items, including polyphenols. These plants are a rich and promising source of natural products and phytochemicals that can be used to treat and prevent numerous diseases and prevent the progression of cancer. Dietary polyphenols exhibit chemo-preventive and therapeutic effects against various ailments, including several types of cancer. The current study focuses on polyphenol’s traditional and advanced extraction methods, with supercritical extraction as a novel approach. It also deals with their identification, bioavailability, and role in preventing and treating colorectal and prostate cancers. Additionally, the article covers the literature that deals with the anticancer activities of polyphenols, as well as their potential use as anticancer agents.

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“…According to our knowledge, there are limited studies in the literature on the cytotoxic activity of styrax liquidus on cancer cells. 21 , 22 In our study, we aimed to research the cytotoxic and apoptotic efficacy of styrax liquidus, an endemic plant derivative whose effects have not been investigated before, on HEp-2 laryngeal cancer and HEK-293 cells (Human Embryonic Kidney-293 cells) with apoptotic pathway and caspase pathway.…”

Section: Introductionmentioning

confidence: 99%

Investigation of Cytotoxic and Apoptotic Effects of Styrax Liquidus Obtained From Liquidambar orientalis Miller (Hamamelidaceae) on HEp-2 Cancer Cell with Caspase Pathway

2023

Eurasian J Med

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Objective: The HEp-2 cell line was first identified as laryngeal cancer cells. Then, it was reported to consist of cervical adenocarcinoma cells derived via HeLa cell line contamination. Styrax liquidus is an exudate that is provided by the injured hull of the Liquidambar orientalis Miller (Hamamelidaceae), which has been used for the treatment of skin problems, peptic ulcers, and parasitic infections or as an antiseptic. In our study, we purposed to research the cytotoxic and apoptotic effect of styrax liquidus on HEp-2 cancer cell line. Materials and Methods: The IC 50 dosage of styrax liquidus (Turkish sweet gum obtained from trees) was set by the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and the apoptotic effect of styrax liquidus on HEp-2 cancer cell was determined by assessing the expression of genes involved in apoptosis ( Bax , Bad , Bak1 , p53 , Bcl-2 , Bcl-XL , Apaf-1 , Caspase2 , Caspase3a , Caspase9 , and Caspase12 ) by quantitative real-time polymerase chain reaction. Results: The IC 50 value of styrax liquidus was found to be 125 μg/mL for 48 hours. According to the results, styrax liquidus reduced the population of HEp-2 laryngeal cancer cells and increased the expression of genes which were apoptosis related. These results indicate that styrax liquidus can be thought as a choice of cancer therapy. Conclusion: The finding of the study showed that it would be more useful to perform more qualified studies about the effect of styrax liquidus on cancer cells.

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The Phytochemical Profile and Biological Activity ofLiquidambar orientalisMill. var.orientalisvia NF-κB and Apoptotic Pathways in Human Colorectal Cancer

Cited by 12 publications

References 42 publications

Effect of Kaempferol on the Biological Behavior of Human Colon Cancer via Regulating MMP1, MMP2, and MMP9

Effect of Kaempferol on the Biological Behavior of Human Colon Cancer via Regulating MMP1, MMP2, and MMP9

Dietary Polyphenols: Extraction, Identification, Bioavailability, and Role for Prevention and Treatment of Colorectal and Prostate Cancers

Investigation of Cytotoxic and Apoptotic Effects of Styrax Liquidus Obtained From Liquidambar orientalis Miller (Hamamelidaceae) on HEp-2 Cancer Cell with Caspase Pathway

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