The Phytoestrogenic Effect of Daidzein in Human Dermal Fibroblasts

Abstract

Abstract:Estrogen deficiency results in a reduction of skin quality and function in postmenopausal women. Over the past decade, many studies have supported that estrogen provides anti-aging effects as a result of the ability of estrogen to prevent skin collagen decline, restore skin elasticity, and increase skin hydration in postmenopausal women skin. Due to their structural similarity with estrogen, isoflavones have been called phytoestrogens. Photoprotective effects of isoflavones are well established while their estrogenic-like activities are not fully understood in human skin. In this study, we investigated whether daidzein, an effective isoflavone, has phytoestrogenic activity and induces transcriptional change of extracellular matrix components in dermal fibroblasts. We examined the luciferase activity of daidzein and β -estradiol using transiently transfected NIH3T3-ERE cells. The estrogenic receptor-dependent transcriptional activity was increased in a dose-dependent manner when treated with daidzein, with a maximum of 2.5-fold induction at 10 µg/mL of daidzein compared with non-treated control. In addition, daidzein significantly in creased the expressions of collagen 1) † 주 저자 (e-mail: misunkim@lgcare.com) 2014 type I, collagen type IV, elastin, and fibrillin-1 in human dermal fibroblasts. By comparing with the effects of β -estradiol through out all the experiments, we confirmed that daidzein had estrogenic activity and function in fibroblasts. These results suggest that daidzein-based application, having both photoprotective and phytoestrogenic effects, may be a powerful approach for skin anti-aging of postmenopausal women.

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“…Many people believe that phytoestrogens can protect the skin from oxidative stress by binding to ERβ and activating ERE, to cause the dissociation of Nrf2 and Keap1. Nrf2 enters the nucleus and activates ARE, which increases the transcription of antioxidant enzymes [39]. This increases mitochondrial membrane potential and stimulates NO release in physiological circumstances.…”

Recent advances in the anti‐aging effects of phytoestrogens on collagen, water content, and oxidative stress

“…Phytoestrogens can protect the skin from oxidative stress: (1) They bind to ERβ and activate ERE to promote the dissociation of Nrf2 and Keap1. Nrf2 enters the nucleus and activates ARE, which enhances the transcription of antioxidant enzymes (Kim et al, ); and (2) They can improve mitochondrial membrane potential and increase NO release under physiological conditions. They can also hinder the decrease in mitochondrial membrane potential and the increased NO release caused by H 2 O 2 .…”

“…This pharmacological activity is attributed to various metabolites including equol and trihydroxy isoflavone (Meng‐Yao et al, ). Kim, Hong, and Lee () investigated whether daidzein, a potent isoflavone, displays phytoestrogen activity and induces transcriptional changes in extracellular matrix components in dermal fibroblasts. The estrogenic receptor‐dependent transcriptional activity increased in a dose‐dependent manner when treated with daidzein, with a maximum of 2.5‐fold induction at 10 μg/mL of daidzein compared with the nontreated control.…”

“…S‐equol binds to ERβ and activates the estrogen response element (ERE), which in turn promotes the dissociation of Nrf2 and Keap1. Nrf2 enters the nucleus and activates the antioxidant response element (ARE), which enhances the transcription of antioxidant enzymes, such as superoxide dismutase (SOD) and glutathione peroxidase (GSH‐Px) (Kim et al, ). As discussed by Jackson et al, equol may increase Nrf2 levels and/or bind to the estrogen‐responsive elements (EREs) in the promoter region the Nrf2 gene and/or increase gene expression of other antioxidant genes (Jackson et al, ).…”

Review: Efektivitas Sari Kedelai sebagai Anti-aging dalam Kosmetik

“…Daidzein berpotensi lebih baik dalam menghambat enzim hyaluronidase dan sebagai antioksidan, memiliki dua mekanisme dalam menghambat radikal bebas yang meningkatkan aktivitas enzim antioksidan [12]. Selain itu, daidzein secara signifikan meningkatkan ekspresi kolagen tipe I, kolagen tipe IV, elastin, dan fibrillin-1 pada fibroblas dermal manusia [36].…”