The PI3K/Akt/mTOR signaling pathway regulates lipid metabolism mediated by endoplasmic reticulum stress in goose primary hepatocytes

Luo, Z.Y.; Song, Qianben; Xiong, X.P.; Abdulai, M.; Liu, H.H.; Li, L.; Xu, H.Y.; Hu, Shuyu; Han*, C.C.

doi:10.1399/eps.2021.325

Cited by 1 publication

(1 citation statement)

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“…SREBP-1c is a key gene in the mTOR pathway and is an essential element of lipid and cholesterol metabolism [27]. C/EBPα can promote its expression, and SREBP-1c can bind to the promoter of PPARγ to activate the expression of PPARγ [28]; adipokine secretion is also related to the TG content of adipocytes. Lipid accumulation leads to decreased ADPN secretion [29].…”

Section: Discussionmentioning

confidence: 99%

miR-15a Targets ABAT Gene to Regulate the Differentiation of Preadipocytes of Yanbian Yellow Cattle

Bai

Fang

et al. 2021

Preprint

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BackgroundMicroRNAs (miRNAs) are small RNAs that cannot be translated into proteins. The length of a mature miRNA is approximately 20-25 nt. Neural regulation, inflammatory response, cell proliferation and apoptosis, fat metabolism, and cell differentiation, among others, have become research hotspots in recent years. In this experiment, dual-luciferase reporter gene detection, qRT-PCR, oil red O staining, western blotting, and other methods were used to select the differentially expressed miR-15a and its target gene, the 4-aminobutyrate aminotransferase (ABAT) gene, in the longissimus muscle tissues of cattle bulls and steers. By transfecting Yanbian yellow cattle precursor adipocytes with equal amounts of artificially synthesised miR-15a mimics, mimics NC, miR-15a inhibitor, and inhibitor NC and inducing differentiation, the total RNA and total protein of cells during the induced differentiation process were extracted.The effect of miR-15a on the mRNA and protein expression of adipogenesis-related genes was analysed. ResultsThe results showed that the lipid droplet content and lipid droplet size of the miR-15a mimic group were significantly lower than those of the mimics NC group, and the mRNA levels of the peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT enhancer-binding protein α (C/EBPα) genes in the miR-15a mimics group were significantly lower than those in the mimics NC group. At the same time, the protein expression of miR-15a mimic group cells was also significantly lower than that of the NC group cells. Meanwhile, the mRNA levels of the PPARγ and C/EBPα genes of miR-15a inhibitor group cells were significantly higher than those of the NC group cells, and the protein expression levels were also significantly higher than those of the NC group cells. The luciferase reporter results showed that miR-15a mimics significantly inhibited the activity of the ABAT-3'UTR dual luciferase reporter gene vector, indicating that the miR-15a and ABAT genes have a targeting effect, and that overexpressed miR-15a significantly reduced the expression of the ABAT gene in the cell, inhibited the expression of miR-15a, and significantly increased the expression of the ABAT.ConclusionsIn summary, miR-15a may target the ABAT gene to regulate the differentiation of Yanbian yellow cattle precursor adipocytes.

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Section: Discussionmentioning

confidence: 99%