The Pig as a Potential Organ Donor for Man

Ye, Y.; Niekrasz, Marek; Kosanke, Stanley D.; Welsh, R.; Jordan, Heinz; Fox, J. Christian; Edwards, W. C.; Maxwell, Christopher A.; Cooper, D. K. C.

doi:10.1097/00007890-199403150-00011

Cited by 111 publications

(31 citation statements)

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“…Transplantation of solid organs and cellular grafts has become a clinical routine over the past two decades [8,9]. Because artificial cells contain almost anything from oxygen to different proteins, this variability of artificial cell contents allows for many possibilities for their uses, such as chronic renal failure, drug poisoning, liver failure, enzyme therapy, metabolic function replacement and tissue engineering [12].…”

Section: Discussionmentioning

confidence: 99%

“…Pigs are the increasingly favoured source of organs for xenotransplantation of organs into humans. Related investigations have suggested using domestic pigs as potential organ source animals [8,9], owing to their reproductive efficiency, comparable organ size parameters and relative similarity with some aspects of human anatomy and physiology. In addition to organ transplantation, porcine materials, for example collagen, small intestine submucosa and pericardial tissue, are appropriate for medical device development [10,11].…”

Section: Introductionmentioning

confidence: 99%

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Application of the Isoenzyme Profile in the Development of Cell-Based Devices

Chang

Chen²,

Liu³

et al. 2001

Acta Biotechnol.

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To detect cell cross-contamination and verify the origin of the cells of an artificial organ, the sensitive isoenzyme assay was chosen to monitor the quality test of cell-based devices. Authoritative cell evaluation of artificial skin products has been established in this study. Human and porcine cell suspensions with total cell counts of between 1 × 10 5 and 4 × 10 6 were individually tested to determine the activity of isoenzymes. Human fibroblast, mixed with 1% to 100% of porcine fibroblast, could be significantly distinguished in the isoenzyme assay. Based on the glucose-6-phosphophatedehydrogenase analysis, the human fibroblast tested in this study belonged to the B type human cells. Lactate dehydrogenase (LD), malate dehydrogenase (MD) and mannose phosphate isomerase isoenzyme (MPI) activities obviously revealed that a different pattern corresponds to the percentage of human and porcine cell mixtures. The discriminatory limit of MPI, LD and MD activity can reach up to 1% of sensitivity of the isoenzyme analysis. This sensitive isoenzyme analysis method allows us to routinely test cellular biomaterials whether interspecies cell line cross-contamination has occurred in the development of artificial organs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Application of the Isoenzyme Profile in the Development of Cell-Based Devices

Chang

Chen²,

Liu³

et al. 2001

Acta Biotechnol.

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“…However, prospective considerations for the types of screening are still necessary (Public Health Service, 2011;Fishman et al, 2012;Fishman, 1994;Ye et al, 1994). However, prospective considerations for the types of screening are still necessary (Public Health Service, 2011;Fishman et al, 2012;Fishman, 1994;Ye et al, 1994).…”

Section: Development Of Spf Herdsmentioning

confidence: 99%

Xenozoonoses

Michaels

2015

Laboratory Animal Medicine

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“…While externally there are many obvious morphological differences between human beings and pigs, their internal anatomy and physiology as well as transplant immunology are very similar [12,13]. The pig is recognized as the best candidate for xenotransplantation.…”

Section: Introductionmentioning

confidence: 99%

A Simplified Technique of Pancreas Transplantation in a Porcine Model

et al. 2014

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Background: The incidence of diabetes has been steadily increasing. The only curative option for diabetes is pancreas transplantation (PTx). Porcine models are valuable because of their anatomical and physiological similarities to human beings. Our aim is to introduce a simplified technique of PTx in a porcine model. Methods: In Landrace pigs (n = 32), after median laparotomy, the pancreas was mobilized, and the portal and splenic veins were divided. The proper hepatic and splenic arteries and the bile duct were also prepared, and the duodenal bulb was prepared and stapled. The third portion of the duodenum was freed up to the ligament of Treitz and stapled, and the renal arteries were ligated. After systemic heparinization, the pancreas was perfused through the abdominal aorta with histidine-tryptophan-ketoglutarate solution. The portal and splenic veins were cut for evaluating the sufficiency of perfusion. Whole pancreaticoduodenal graft was procured along with an aortic jump graft containing mesenteric and celiac trunks. In recipients, after total pancreatectomy, the suprarenal inferior vena cavaand infrarenal aorta were prepared for vascular anastomosis in an end-to-side manner. After pancreas reperfusion, duodenoduodenostomy was performed in an end-to-side manner. Results: Median cold and warm ischemia times were 10 h (range, 9-14 h) and 50 min (range, 35-80 min), respectively. The hemodynamic status was stable throughout the operation. The median follow-up period was 7 days (range, 4-10). There were no major intra- and postoperative complications. Conclusion: By using an aortic jump graft, there was no need to perform additional arterial reconstruction resulting in a short warm ischemic and operation time. End-to-side portocaval and duodenoduodenal anastomoses make this model of PTx a very feasible method for experimental evaluations.

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The Pig as a Potential Organ Donor for Man

Cited by 111 publications

References 0 publications

Application of the Isoenzyme Profile in the Development of Cell-Based Devices

Application of the Isoenzyme Profile in the Development of Cell-Based Devices

Xenozoonoses

A Simplified Technique of Pancreas Transplantation in a Porcine Model

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