The pig histocompatibility system SLA: Serological study on a group of antigenic specificities

Abstract

SummaryThe report presents an analysis of a group of class I SLA reagents which were highly correlated within one cluster in a previous analysis. Further population and family studies, and selected purification of several of these reagents led to the identification of 4 distinct specificities, namely SLA A 15, B 18, C 1 and A 16. Three of them, SLA A 15, B 18 and C 1, are actually in strong linkage disequilibrium and represent the main SLA haplotype in the Large White breed. SLA A 16 is present essentially in the Landrace breeds. SLA A 16 displays a strong cross‐reaction with SLA A 15 and there is another specificity in linkage disequilibrium with SLA A 16 which cross‐reacts with SLA B 18. Altogether, the strong linkage disequilibrium and the high degree of cross‐reactivity among the allelic products of the SLA complex explain the failure to detect the diversity of our reagents in previous studies.

Molecular characterization of swine leucocyte antigen class I genes in outbred pig populations

“…Their high-resolution equivalents, Hp-1.0 and Hp-4.0, have been observed in multiple commercial and miniature pig breeds (Smith et al 2005b;Ho et al 2009). This high prevalence is also consistent with previous observations that their serologic equivalents, H01/H28 and H04, respectively, were very common in many commercial pig breeds such as the Large White, Landrace and Duroc (Renard et al 1982(Renard et al , 1988. The serologic equivalent of Lr-21.0, H03, was also frequently observed in commercial pig breeds.…”

Detection of a new non‐MHC alloantigenic system (SLD) in pigs

“…SLA, SLB and SLD antigens were detected by means of a standard lymphocytotoxicity test (Vaiman, Hauptmann & Mayer, 1978). A mixture of rabbit sera absorbed with spleen cells or lymphocytes from lymphnodes was used as the complement source (Renard, Chardon & Vaiman, 1982). SLC antigens were detected by the microleucoagglutination technique described by van Rood, van Leeuwen & Zveerus (1970).…”