The Pinking Shears

Joyce, Christopher; Joyce, Kieran; Jones, Deirdre

doi:10.1097/gox.0000000000000227

Cited by 3 publications

(1 citation statement)

References 2 publications

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“…To date, tissue-engineered scaffolds have been widely used to regenerate damaged tissues and organs owing to their diverse advantageous features such as [1] biocompatibility, to prevent rejection response after implants [2]; an appropriate surface chemistry to enhance cell attachment and proliferation [3]; an interconnected pore structure to allow cell infiltration into the vessel [4]; sufficient biodegradability to allow the formation of new tissue; and [5] suitable mechanical properties to maintain the structure [18][19][20]. For example, skin scaffolds (termed as skin substitutes or artificial dermis) including INSUREGRAF ® (South Korea), Matriderm ® (Germany), Pelnac ® (UK), and Integra ® (USA) have been developed as skin wound dressings and applied clinically for eventual scar-free wound healing [21,22]. In particular, the recently developed artificial dermis INSUREGRAF ® is composed of collagen, which has multiple desirable characteristics such as biocompatibility, an interconnected pore structure, and sufficient biodegradability, and is consequently able to regenerate skin tissue as a onestage dermal substitute (OSDS) operation in patients with full-thickness (FT) skin defects [23].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of wound healing efficiency mediated by artificial dermis functionalized with EGF or NRG1

Yoon

Cha

et al. 2018

Biomed. Mater.

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The use of artificial dermis as a skin substitute is a field of active study, as acellular dermal matrices from cadavers are susceptible to infection owing to their human origin. One such alternative dermal replacement scaffold, INSUREGRAF, is derived primarily from extracellular matrix proteins such as collagen and elastin and has been clinically used to treat severe skin wounds such as burns. This scaffold has proven to be useful to minimize wound contraction and scar formation owing to its biocompatibility, interconnected pore structure, sufficient biodegradability, and suitable mechanical properties. However, INSUREGRAF does not provide scar-free wound healing in cases of severe skin damage such as full-thickness (FT) excision. Considering that the efficient recruitment of fibroblasts and keratinocytes into a wound site represents a critical step in the regeneration of damaged skin, we attempted to enhance the efficiency for wound healing by fabricating growth factor-functionalized INSUREGRAF. In particular, we utilized epidermal growth factor (EGF) and an EGF family member, neuregulin-1 (NRG1), not previously studied in the context of wound healing, whose cellular role is to promote proliferation and migration in fibroblasts and keratinocytes. Both artificial dermis-growth factor combinations led to efficient recruitment of fibroblasts and keratinocytes into a wound site during the early steps of skin regeneration. Notably, EGF- or NRG1-functionalized INSUREGRAF induced rapid proliferation of skin cells in an ERK pathway-dependent manner and exhibited efficient wound healing in a Sprague-Dawley rat FT excision and grafting model. These results provide the foundation for expanding the use of growth factor-functionalized INSUREGRAF to clinical application in cases of severe skin injury.

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Section: Introductionmentioning

confidence: 99%

Enhancement of wound healing efficiency mediated by artificial dermis functionalized with EGF or NRG1

Yoon

Cha

et al. 2018

Biomed. Mater.

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Our initial learning curve in the enzymatic debridement of severely burned hands—Management and pit falls of initial treatments and our development of a post debridement wound treatment algorithm

Schulz¹,

Perbix²,

Shoham

et al. 2017

Burns

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Epithelial differentiation of human adipose-derived stem cells (hASCs) undergoing three-dimensional (3D) cultivation with collagen sponge scaffold (CSS) via an indirect co-culture strategy

Gao

et al. 2020

Stem Cell Res Ther

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Background: Three-dimensional (3D) cultivation with biomaterials was proposed to facilitate stem cell epithelial differentiation for wound healing. However, whether human adipose-derived stem cells (hASCs) on collagen sponge scaffold (CSS) better differentiate to keratinocytes remains unclear. Methods: 3D cultivation with CSS on hASC epidermal differentiation co-cultured with HaCaT cells at air-liquid interface (ALI) was compared with two-dimensional (2D) form and cultivation without "co-culture" or "ALI." Cellular morphology, cell adhesion, and growth condition were evaluated, followed by the protein and gene expression of keratin 14 (K14, keratinocyte specific marker). Results: Typical cobblestone morphology of keratinocytes was remarkably observed in co-cultured hASCs at ALI, but those seeded on the CSS exhibited more keratinocyte-like cells under an invert microscope and scanning electron microscope. Desired cell adhesion and proliferation were confirmed in 3D differentiation groups by rhodamine-labeled phalloidin staining, consistent with H&E staining. Compared with those cultured in 2D culture system or without "ALI," immunofluorescence staining and gene expression analysis revealed hASCs co-cultured over CSS expressed K14 at higher levels at day 15. Conclusions: CSS is positive to promote epithelial differentiation of hASCs, which will foster a deeper understanding of artificial dermis in skin wound healing and regeneration.

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The Pinking Shears

Cited by 3 publications

References 2 publications

Enhancement of wound healing efficiency mediated by artificial dermis functionalized with EGF or NRG1

Enhancement of wound healing efficiency mediated by artificial dermis functionalized with EGF or NRG1

Our initial learning curve in the enzymatic debridement of severely burned hands—Management and pit falls of initial treatments and our development of a post debridement wound treatment algorithm

Epithelial differentiation of human adipose-derived stem cells (hASCs) undergoing three-dimensional (3D) cultivation with collagen sponge scaffold (CSS) via an indirect co-culture strategy

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