The piscine micronucleus test to assess the impact of pollution on the Japaratuba river in Brazil

Abstract

In situ investigations of the effects of mutagenic pollutants (environmental monitoring) have increasingly used bioindicators, and fish often have been used in these studies as sentinel organisms. In the present study, we have used the piscine micronucleus test (MNT) as an in situ biological indicator of chemical contamination in two fresh water fish species (Astyanax bimaculatus and Hoplias malabaricus). The fish were collected from two sites (Sites 1 and 2) in the Japaratuba River (Sergipe, Brazil), in an area impacted by a petrochemical industrial complex which indirectly contributes treated effluent (produced water) to the river. Responses in fish from these sites were compared to fish from a clean reference site (Jacarecica River - Sergipe, Brazil). The results indicated an enhanced frequency of micronuclei (MN) in peripheral red blood cells of A. bimaculatus collected at Sites 1 and 2 when compared to their respective negative control (A. bimaculatus collected from the Jacarecica River). H. malabaricus collected at Sites 1 and 2 did not have a significant increase in MN. The results of this study indicate that the piscine MNT is a useful in vivo technique for the detection of chemical contaminants in the aquatic environment and that the assay shows potential for in situ monitoring of water quality. Nevertheless, the results also demonstrated differential sensitivity of A. bimaculatus and H. malabaricus to the induction of MN.

Digital light microscopy as a tool in toxicological evaluation of fish erythrocyte morphological abnormalities

“…Appearance of MN was another prominent characteristic observed in blood cell films. Presence of erythrocyte with alteration nuclear morphology was reported also by other authors as physiologic conditions (Arikan, Goçmen, Atatur, Kumlutas, & Çiçek, ; Claver & Quaglia, ; Mayer, Knoll, Innis, & Mitchell, ), or induced by urban or industrial pollutant such as petrochemical products (Barbee et al, ; Marlasca, Sanpera, Riva, Sala, & Crespo, ), heavy metals (De Moraes Pantaleao, Alcantara, Hora Alves Ido, & Spano, ; Moraes de Andrade et al, ) and pesticides (De Lemos, Iranco Fde, de Oliveira, de Souza, & Fachel, ; Moraes de Andrade et al, ).…”

DNA Damage of Erythrocytes of Fish Oreochromis niloticus (Perciformes, Cichlidae), After Acute Exposure to River Water Receiving Effluent From an Oil Refinery

“…The same sampling points were analyzed previously by Souza & Fontanetti (2006) a Categories of the migration of comets: 0 -3 (Rigonato et al, 2005) Average damage score: average ± standard deviation effects of waste from the petrochemical industry on the genetic material of aquatic organisms. Pantaleão et al (2006) monitored the Japaratuba River using the MN test in two fish species: Hoplias malabaricus and Astyanax bimaculatus. This river receives the wastewater from a petrochemical complex.…”

“…Recently, the effectiveness of the comet assay in erythrocytes of several species of fish has been proven (Russo et al, 2004;Çavas & Kopen, 2008;Christofoletti et al, 2009). Several papers have reported an association between DNA damage and mutagenicity in fish and rivers affected by refinery effluents (Çavas, & Ergene-Gözükara, 2005;Hoshina et al, 2008) or influenced by the petrochemical complex (Pantaleão et al, 2006;Lemos et al, 2007 and.…”

High waterborne Mg does not attenuate the toxic effects of Fe, Mn, and Ba on Na+ regulation of Amazonian armored catfish tamoatá (Hoplosternum litoralle)

“…In general, formation water has high concentrations of soluble salts and metals (Woodall et al 2003;Jackson and Reddy 2007;Manfra et al 2007;Baldisserotto et al 2012). The composition of formation Responsible editor: Philippe Garrigues water is distinguished among the production fields but it is commonly constituted by high levels of iron (Fe) and manganese (Mn) (Woodall et al 2003;Pantaleão et al 2006;Manfra et al 2007;Baldisserotto et al 2012), and in some areas also by high barium (Ba) levels (Jackson and Reddy 2007;Baldisserotto et al 2012). In this context, the formation water extracted from the Urucu platform, located near the Urucu River (Amazon, Brazil), contains very high levels of ions, Fe, Mn, and Ba (Baldisserotto et al 2012).…”