“…Vital staining of nerve fibres can be achieved only with Methylene Blue and related N ‐methylated cationic thiazine dyes [142,143]. Either the dye or its colourless and more hydrophobic leuco compound may be used; advantages are claimed for both [138,144]. Adequate oxygenation is necessary in order to see the stained cells in the fresh state, but the dye can be immobilised in situ by a suitable precipitating anion; ammonium molybdate is traditional [145], but advantages are claimed for recent variants of the technique that include treating the stained preparations with glutaraldehyde and PMA [140,146,147].…”