The Planctomycetia: an overview of the currently largest class within the phylum Planctomycetes

Vitorino, Inês; Lage, Olga Maria

doi:10.1007/s10482-021-01699-0

Cited by 35 publications

(34 citation statements)

References 149 publications

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“…Strain ICM_H10 T had its origin in a typical marine coastal environment, namely in a rocky beach located on the Portuguese north coast. This is in line with the currently known ecology of the family Planctomycetaceae: most strains isolated until the present time were retrieved from marine environments, excluding a few genera such as Planctopirus, Planctomicrobium and Schlesneria [30].…”

Section: Isolation and Ecologysupporting

confidence: 81%

“…A complete set of genes coding for glycolytic enzymes shows the presence of a fully functional glycolysis (Embden–Meyerhof–Parnas) pathway in strain ICM_H10 T , as well as the key enzymes for the tricarboxylic acid cycle (Table S1). These pathways are commonly present and complete in most Planctomycetaceae [30]. This strain has the genes necessary for the conversion of β- d -glucose-6P to d -Ribulose, in the pentose phosphate pathway; however, for gluconeogenesis, only a partial set of genes was present.…”

Section: Genome Featuresmentioning

confidence: 99%

“…Although they are widespread, the number of planctomycetes in pure culture is still very low when compared to other bacterial groups. The number of species currently formally described in the whole phylum is less than 150, with the class Planctomycetia being the most-studied class [30]. A quick search into the silva SSU Ref NR database (release 138.1 from 27 August 2020) [31] shows that the majority of the full-length 16S rRNA gene non-redundant sequences (defined by a 99 % identity threshold) assigned to the phylum belong to organisms that have not yet been cultivated.…”

Section: Introductionmentioning

confidence: 99%

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Rubinisphaera margarita sp. nov., a novel planctomycete isolated from marine sediments collected in the Portuguese north coast

Vitorino

Lobo‐da‐Cunha

Vasconcelos

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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The phylum Planctomycetota is constituted by bacteria with unique features that are well adapted to a vast range of habitats. Here, we describe a novel planctomycete isolated from marine sediments collected on a beach in Matosinhos (Portugal) using an iChip-based culturing technique. Strain ICM_H10T forms beige-coloured colonies in modified M14 medium and its cells are spherical to ovoid in shape, stalked, rosette-forming and showing motility in a phase of the life cycle. Transmission electron microscopy observations showed a typical planctomycetal cell plan and cell division by budding. This strain requires salt for growth and grows in the range of 2.0–5.0 % (w/v) NaCl, from 20 to 37 °C, within a pH of 6.0–9.0 and is able to use diverse nitrogen and carbon sources. It is heterotrophic, aerobic and capable of microaerobic growth. This strain has a genome size of approximately 6.0 Mb and a G+C content of 58.1 mol%. A 16S rRNA gene-based phylogenetic analysis supports the association of strain ICM_H10T to the phylum Planctomycetota and the family Planctomycetaceae , as it shares only 96.8 and 96.4% similarity to its closest relatives Rubinisphaera italica Pan54T and Rubinisphaera brasiliensis IFAM 1448T, respectively. Other phylogenetic markers also support the separation of this strain into a novel species. Morphological, physiological and genomic comparisons between strain ICM_H10T and its closest relatives strongly suggest that ICM_H10T represents a new species of the genus Rubinisphaera , for which we propose the name Rubinisphaera margarita sp. nov., with ICM_H10T (=CECT 30326T=LMG 32234T) as type strain.

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Section: Isolation and Ecologysupporting

confidence: 81%

Section: Genome Featuresmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rubinisphaera margarita sp. nov., a novel planctomycete isolated from marine sediments collected in the Portuguese north coast

Vitorino

Lobo‐da‐Cunha

Vasconcelos

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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“…The first described species in the family was Bythopirellula goksoeyrii [25, 26] and the type genus/species is Lacipirellula/Lacipirellula parvula [24]. In the last 2 years, additional novel taxa were described and the family currently comprises 13 species and 8 genera [22]: Bythopirellula , Lacipirellula , Aeoliella , Posidoniimonas , Botrimarina , Adhaeretor , Pseudobythopirellula and Pirellulimonas [24–29]. These include freshwater species (genus Lacipirellula ) [24, 29] and marine-associated ones, retrieved from a variety of environments such as hydrothermal areas, seawater, iron-hydroxide deposits and associated with the biofilms of Posidonia oceanica and the alga Laminaria sp.…”

Section: Introductionmentioning

confidence: 99%

“…The phylum is presently constituted by the classes Planctomycetia and Phycisphaerae [19, 20], with a total of below 200 different species described. Additionally, the proposed class 'Candidatus Brocadiia' is constituted by planctomycetes that are capable of anaerobic ammonium oxidation (anammox) [21].Planctomycetota seem to be distributed around the globe and in many different habitats, either marine, freshwater, terrestrial or extreme [4,5,22]. For many years, two lineages of Pirellula-related planctomycetes, designated as 'Pir4' and 'Pir 3' , remained uncultivated [23], although these clades were shown to be particularly widespread in anaerobic and low-oxygen environments [24].…”

mentioning

confidence: 99%

Aeoliella straminimaris sp. nov., a novel member of the phylum Planctomycetota with an unusual filamentous structure

Vitorino

Lobo‐da‐Cunha

Vasconcelos

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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Organisms with distinctive biological features and cellular organization constitute the bacterial phylum Planctomycetota . In this study, we formally describe a novel isolate, strain ICT_H6.2T, isolated from sediment samples collected in the brackish environment of the Tagus River estuary (Portugal) using an iChip-based culturing technique. The 16S rRNA gene analysis placed this strain into the phylum Planctomycetota and family Lacipirellulaceae , with a similarity value of 98.0 % to its closest relative Aeoliella mucimassa Pan181T, the currently only known member of the genus. Strain ICT_H6.2T has a genome size of 7.8 Mbp and a DNA G+C content of 59.6 mol %. Strain ICT_H6.2T is heterotrophic, aerobic and capable of microaerobic growth. This strain grows from 10 to 37 °C and from pH 6.5 to 10.0, requires salt to grow, and can tolerate up to 4 % (w/v) NaCl. Diverse nitrogen and carbon sources are utilized for growth. Morphologically, strain ICT_H6.2T is white to beige pigmented, spherical to ovoid in shape and around 1.4×1.1 µm in size. The strain clusters mainly in aggregates and younger cells show motility. Ultrastructural studies showed a cell plan with cytoplasmatic membrane invaginations and unusual filamentous structures with hexagonal organization in transversal section. Morphological, physiological and genomic comparison between strain ICT_H6.2T and its closest relatives strongly suggests it represents a novel species within the genus Aeoliella , for which we propose the name Aeoliella straminimaris sp. nov., represented by strain ICT_H6.2T as the type strain (=CECT 30574T=DSM 114064T).

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Adaptive traits of Planctomycetota bacteria to thrive in macroalgal habitats and establish mutually beneficial relationship with macroalgae

Gao,

Xiao,

Wang

et al. 2024

Limnol Oceanogr Letters

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Bacteria and macroalgae share an inseparable relationship, jointly influencing coastal ecosystems. Within macroalgae habitats, Planctomycetota, a group of bacteria notoriously challenging to cultivate, often dominate. However, the mechanisms facilitating their persistence in this environment remain unclear. Here, we successfully isolated a novel Planctomycetota bacterium, Stieleria sp. HD01, from the surface of kelp. We demonstrated that HD01 possesses a robust ability to metabolize fucoidan, which constitutes half of the kelp‐derived organic carbon and exhibits resistance to attack by most microorganisms. Moreover, HD01 can utilize a broad spectrum of other organics, indicating its metabolic versatility and competitive prowess within algal environments. Additionally, HD01 can secrete antagonistic substances against other bacteria, form biofilms, and employ superoxide dismutase and catalase to resist oxidative stress, further consolidating its ecological fitness. Comparative metagenomics analysis suggested that Planctomycetota may have a mutually beneficial relationship with kelp.

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The Planctomycetia: an overview of the currently largest class within the phylum Planctomycetes

Cited by 35 publications

References 149 publications

Rubinisphaera margarita sp. nov., a novel planctomycete isolated from marine sediments collected in the Portuguese north coast

Rubinisphaera margarita sp. nov., a novel planctomycete isolated from marine sediments collected in the Portuguese north coast

Aeoliella straminimaris sp. nov., a novel member of the phylum Planctomycetota with an unusual filamentous structure

Adaptive traits of Planctomycetota bacteria to thrive in macroalgal habitats and establish mutually beneficial relationship with macroalgae

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