The plant NADPH oxidase RBOHD is required for microbiota homeostasis in leaves

Pfeilmeier, Sebastian; Petti, Gabriella C.; Bortfeld‐Miller, Miriam; Daniel, Benjamin J.; Field, Christopher M.; Sunagawa, Shinichi; Vorholt, Julia A.

doi:10.1038/s41564-021-00929-5

Cited by 108 publications

(155 citation statements)

References 101 publications

Supporting

Mentioning

107

Contrasting

Unclassified

Order By: Relevance

“…The interplay between the plant innate immune system and the plant microbiota recently emerged as a key mechanism that maintains host–microbe homeostasis for plant health ( 13 , 26 – 30 ). Dysbiosis is characterized by a disruption of this equilibrium caused by an imbalance in the microbiota, which in turn directly affect plant health.…”

mentioning

confidence: 99%

Tryptophan metabolism and bacterial commensals prevent fungal dysbiosis in Arabidopsis roots

Wolinska

Vannier

Thiergart

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

In nature, roots of healthy plants are colonized by multikingdom microbial communities that include bacteria, fungi, and oomycetes. A key question is how plants control the assembly of these diverse microbes in roots to maintain host–microbe homeostasis and health. Using microbiota reconstitution experiments with a set of immunocompromised Arabidopsis thaliana mutants and a multikingdom synthetic microbial community (SynCom) representative of the natural A. thaliana root microbiota, we observed that microbiota-mediated plant growth promotion was abolished in most of the tested immunocompromised mutants. Notably, more than 40% of between-genotype variation in these microbiota-induced growth differences was explained by fungal but not bacterial or oomycete load in roots. Extensive fungal overgrowth in roots and altered plant growth was evident at both vegetative and reproductive stages for a mutant impaired in the production of tryptophan-derived, specialized metabolites (cyp79b2/b3). Microbiota manipulation experiments with single- and multikingdom microbial SynComs further demonstrated that 1) the presence of fungi in the multikingdom SynCom was the direct cause of the dysbiotic phenotype in the cyp79b2/b3 mutant and 2) bacterial commensals and host tryptophan metabolism are both necessary to control fungal load, thereby promoting A. thaliana growth and survival. Our results indicate that protective activities of bacterial root commensals are as critical as the host tryptophan metabolic pathway in preventing fungal dysbiosis in the A. thaliana root endosphere.

show abstract

mentioning

confidence: 99%

Tryptophan metabolism and bacterial commensals prevent fungal dysbiosis in Arabidopsis roots

Wolinska

Vannier

Thiergart

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Beyond bacteria, archaeal gut communities have been analysed across 110 vertebrate species 17 , revealing co-phylogeny, especially for mammalian herbivores. And beyond vertebrate hosts, a study characterizing the leaf microbiome (or phyllosphere) uncovered an essential plant gene for microbiota homeostasis 18 , encoding the plant NADPH oxidase RBOHD.…”

mentioning

confidence: 99%

2021 in review

2021

Nat Microbiol

View full text Add to dashboard Cite

“…DC3000 (Wang et al, 2016). Reduced growth in the host plants is associated with dysbiosis which has been reported in the phyllosphere (Chen T. et al, 2020;Pfeilmeier et al, 2021). The discovery of phyllospheric dysbiosis is a recently discovered phenomenon and, consequently, defined characteristics of dysbiosis are still emerging.…”

Section: Discussionmentioning

confidence: 99%

Salicylic Acid-Mediated Disturbance Increases Bacterial Diversity in the Phyllosphere but Is Overcome by a Dominant Core Community

et al. 2022

View full text Add to dashboard Cite

Plant microbiomes and immune responses have coevolved through history, and this applies just as much to the phyllosphere microbiome and defense phytohormone signaling. When in homeostasis, the phyllosphere microbiome confers benefits to its host. However, the phyllosphere is also dynamic and subject to stochastic events that can modulate community assembly. Investigations into the impact of defense phytohormone signaling on the microbiome have so far been limited to culture-dependent studies; or focused on the rhizosphere. In this study, the impact of the foliar phytohormone salicylic acid (SA) on the structure and composition of the phyllosphere microbiome was investigated. 16S rRNA amplicons were sequenced from aerial tissues of two Arabidopsis mutants that exhibit elevated SA signaling through different mechanisms. SA signaling was shown to increase community diversity and to result in the colonization of rare, satellite taxa in the phyllosphere. However, a stable core community remained in high abundance. Therefore, we propose that SA signaling acts as a source of intermediate disturbance in the phyllosphere. Predictive metagenomics revealed that the SA-mediated microbiome was enriched for antibiotic biosynthesis and the degradation of a diverse range of xenobiotics. Core taxa were predicted to be more motile, biofilm-forming and were enriched for traits associated with microbe-microbe communication; offering potential mechanistic explanation of their success despite SA-mediated phyllospheric disturbance.

show abstract

The plant NADPH oxidase RBOHD is required for microbiota homeostasis in leaves

Cited by 108 publications

References 101 publications

Tryptophan metabolism and bacterial commensals prevent fungal dysbiosis in Arabidopsis roots

Tryptophan metabolism and bacterial commensals prevent fungal dysbiosis in Arabidopsis roots

2021 in review

Salicylic Acid-Mediated Disturbance Increases Bacterial Diversity in the Phyllosphere but Is Overcome by a Dominant Core Community

Contact Info

Product

Resources

About